Abstract
An efficient protocol for spinach (Spinacia oleracea L.) plant regeneration from hypocotyl and root segments was established. When the sub-apical hypocotyl and tip-free root segments were cultured on Murashige & Skoog (1962)-based medium containing high concentrations of indole-3-acetic acid (85.62 μM) and gibberellic acid (100 μM), more than 75% and 90% of the hypocotyl and root explants, respectively, formed shoots. After elongation, more than 92% of the shoots rooted on medium supplemented with 2.85–5.71 μM of indole-3-acetic acid. More than 70% of rooted plantlets survived in soil and were fertile. Significant interactions between growth regulator combinations, explant types and environmental conditions on shoot initiation, development and rooting were discussed.
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Abbreviations
- BA:
-
benzyladenine
- BM:
-
Murashige & Skoog basal medium
- B5:
-
Gamborg et al. medium (1968)
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
- 2ip:
-
isopentenyladenine
- GA3 :
-
gibberellic acid
- IAA:
-
indole-3-acetic acid
- MS:
-
Murashige & Skoog medium (1962)
- NAA:
-
naphthaleneacetic acid
- HS:
-
hypocotyl segments
- RSS:
-
root segments of seedlings
- RSV:
-
foot segments of in vitro plantlets
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Xiao, Xg., Branchard, M. In vitro high frequency plant regeneration from hypocotyl and root segments of spinach by organogenesis. Plant Cell Tiss Organ Cult 42, 239–244 (1995). https://doi.org/10.1007/BF00029993
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DOI: https://doi.org/10.1007/BF00029993