Abstract
The production of fructose-containing carbohydrates by leaf and node callus of Gomphrena macrocephala St.-Hil. grown in three different auxin to cytokinin ratios is described. The amount of these carbohydrates in node callus rose with increasing α-naphthalene acetic acid to 6-benzylaminopurine ratios, while in leaf callus it tended to decrease. An homologous series of fructans was detected only in callus grown in 1:2 auxin to cytokinin ratio. These fructans were of the inulin series in contrast to the phlein series present in the tuberous root of intact plants. Fructose polymers were also detected in culture medium and these made up approximately 98% of the total fructopolysaccharides produced. The mean molecular weight of these polymers (40 kDa) was 3 times greater than that of fructan from intact plants. The fructo-polysaccharide pool could be a reflection of different physiological processes taking place in the callus rather than a direct effect of growth regulator treatment.
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Abbreviations
- BA-6:
-
benzylaminopurine
- DP:
-
degree of polymerization
- high-MW:
-
high molecular weight
- HPAEC/PAD:
-
high performance anion-exchange chromatography with pulsed amperometric detection
- low-MW:
-
low molecular weight
- MS:
-
Murashige and Skoog
- NAA:
-
α-naphthalene acetic acid
- TFA:
-
trifluoroacetic acid
- TLC:
-
thin layer chromatography
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Vieira, C.C.J., Braga, M.R. & Figueiredo-Ribeiro, R.C.L. Fructans in callus of Gomphrena macrocephala St.-Hil.. Plant Cell Tiss Organ Cult 42, 233–238 (1995). https://doi.org/10.1007/BF00029992
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DOI: https://doi.org/10.1007/BF00029992