Abstract
Regrowth after encapsulation in a sodium alginate matrix of micropropagated buds from six different in vitro proliferated woody species was evaluated. Actinidia deliciosa Liang & Ferguson (kiwifruit), Betula pendula Roth (birch), Crataegus oxyacantha L. (hawthorn), Malus spp. (apple), Rubus spp. (blackberry) and Rubus idaeus L. (raspberry) propagated in vitro were used as bud sources. Encapsulation with sodium alginate and subsequent regrowth on nutrient rich medium was compared to encapsulation with nutrient-enriched alginate capsules followed by regrowth on nutrientless medium. Apical and sub-apical buds of Malus (rootstock ‘M. 27’ and cultivar ‘Starkspur Red’) were also compared for encapsulation and regrowth ability. All species showed a regrowth after encapsulation, but only if cultured on enriched media. ‘M.27’ apical and sub-apical buds showed different regrowth ability after encapsulation with sodium alginate. Applicability of encapsulation of single micropropagated tree buds is discussed.
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References
Accart F, Monod V, Poissonnier M, Dereuddre J & Paques M (1994) Cryopreservation of Populus alba×tremula shoot tips in vitro cultured. Abstracts of the VIIIth International Congress of Plant Tissue and Cell Culture, Firenze, Italy, 12–17 June: 51
Agostoni F & Marinoni CM (1987) Manuale di progettazione di spazi verdi. Nicola Zanichelli Editore S.p.A., Bologna, Italy
Bapat VA (1993) Studies on synthetic seeds of sandalwood (Santalum album L.) and mulberry (Morus indica L.). In: Redenbaugh K (Ed) Synseeds: Applications of Synthetic Seeds to Crop Improvement (pp 381–408). CRC Press Inc., Boca Raton, Fl, USA
Barbotin JN, Nava Saucedo EJ, Bazinet C, Kersulec A, Thomasset B & Thomas D (1993) Immobilization of whole cells and somatic embryos: coating process and cell-matrix interactions. In: Redenbaugh K (Ed) Synseeds: Applications of Synthetic Seeds to Crop Improvement (pp 65–103), CRC Press Inc., Boca Raton, Fl, USA
Bounous G (1993) Situazione, tendenza e prospettive della coltivazione dei piccoli frutti in Italia. Frutticoltura 11: 29–38
Costa G, Testolin R & Biasi R (1992) Situazione attuale e prospettive della coltura dell'actinidia in Italia. Frutticoltura 9: 7–16
Fabre J & Dereuddre J (1990) Encapsulation-dehydration: a new approach to cryopreservation of Solanum shoot tips. Cryo-letters 11: 413–426
Gamborg O, Miller R & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cells Res. 50: 151–158
Ganapathi TR, Suprasanna P, Bapat VA & Rao PS (1992) Propagation of banana through encapsulated shoot tips. Plant Cell Rep. 11: 571–575
Gautheret RS (1959) La culture des tissus végétaux. Ed. Masson, Paris (France)
Jacoboni A & Standardi A (1982) La moltiplicazione in vitro del melo cv. Wellspur. Rivista dell' Ortoflorofrutt. It. 3: 217–229
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Mathur J, Ahuja PS, Lai N & Mathur AK (1989) Micropropagation of Valeriana wallichii D.C. using encapsulated apical and axial shoot buds. Plant Science 60: 111–116
Quoirin M & Lepoivre P (1977) Etude de milieux adaptés aux cultures in vitro de Prunus. Acta Hortic. 78: 437–442
Redenbaugh K, Fujii JAA & Slade D (1993) Hydrated coatings for synthetic seed. In: Redenbaugh K (Ed) Synseeds: Applications of Synthetic Seeds to Crop Improvement (pp 35–46). CRC Press Inc., Boca Raton, Fl, USA
Standardi A & Catalano F (1984) Tissue culture propagation of kiwifruit. The International Plant Propagators' Society Vol. 34: 236–243
Walkej DGA (1972) Production of apple plantlets from axillary bud meristems. Can. J. Plant Sci. 52: 1085–1087
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Piccioni, E., Standardi, A. Encapsulation of micropropagated buds of six woody species. Plant Cell Tiss Organ Cult 42, 221–226 (1995). https://doi.org/10.1007/BF00029990
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DOI: https://doi.org/10.1007/BF00029990