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Effect of exo-16,17-dihydro-gibberellin A5 on gibberellin A20 metabolism in seedlings of dwarf rice (Oryza sativa L. cv. Tan-ginbozu)

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Abstract

Several of the 16,17-dihydro gibberellins (GAs) inhibit elongation in a variety of species. In a study of their mechanism of action we have investigated the effect of exo-16,17-dihydro-Ga5 (diHGA5) on the metabolism of GA20 in dwarf rice (Oryza sativa cv. Tan-ginbozu). A mixture of [3H]- and [3H]-GA20 (100 ng per plant) was applied in microdrops to 4 d old seedlings which were harvested 72 h later. Concurrent treatment with diHGA5 at 100 ng or 333 ng per plant reduced GA20-induced elongation of the second leaf sheath by 41–66%. There was a concomitant reduction in the amount of [2H2]GA1 present at harvest, measured by gas chromatography-mass spectrometry-selected ion monitoring. The [2H2]GA29 content was also reduced. There was no clear effect of diHGA5 on the total radioactivity recovered, or on conversion of the [3H]GA20 to putative [3H]GA conjugates, or on the amount of [2H2]GA20 found. No free [2H2]GA8 was detected. In other experiments there was little effect of diHGA5 on elongation induced by treatment with GA1. We conclude that diHGA5 inhibited GA20-induced elongation in dwarf rice shoots at least partly by reducing the 3β-hydroxylation of GA20 to GA1.

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Abbreviations

diHGA5 =:

exo- 16, 17-dihydro-gibberellin A5

GA =:

gibberellin

GC-MS-SIM =:

gas chromatography-mass spectrometry-selected ion monitoring

References

  1. Adams R, Kerber E, Pfister K and Weiler EW (1992) Studies on the action of the new growth retardant CGA 163'935 (Cimectacarb). In: Karssen CM, van Loon LC and Vreugdenhil D (eds) Progress in Plant Growth Regulation, pp 818–827. Dordrecht: Kluwer

    Google Scholar 

  2. Brian PW, Grove JF and Mulholland TPC (1967) Relationships between structure and growth-promoting activity of the gibberellins and some allied compounds, in four test systems. Phytochemistry 6: 1475–1499

    Article  Google Scholar 

  3. Evans LT, King RW, Mander LN, Pearce DW and Pharis RP (1991) Method of treating plants or plant tissues. UK Patent Application #9117634.7; European Patent Application No. 92917726.9; International Patent Application No. PCT/AU92/00426

  4. Evans LT, King RW, Mander LN, Pharis RP and Duncan KA (1994) The differential effects of C-16,17-dihydro gibberellins and related compounds on stem elongation and flowering in Lolium temulentum. Planta (In press)

  5. Hedden P (1991) Gibberellin biosynthetic enzymes and the regulation of gibberellin concentration. In: Takahashi N, Phinney BO and MacMillan J (eds) Gibberellins, pp 94–105. New York: Springer-Verlag

    Google Scholar 

  6. Graebe JE, Lange T, Pertsch S and Stöckl D (1991) The relationship of different gibberellin biosynthetic pathways in Curcurbita maxima endosperm and embryos and the purification of a C-20 oxidase from the endosperm. In: Takahashi N, Phinney BO and MacMillan J (eds) Gibberellins, pp 51–61. New York: Springer-Verlag

    Google Scholar 

  7. Kamiya Y, Nakayama I and Kobayashi M (1992) Useful probes to study the biosynthesis of gibberellins. In: Karssen CM, van Loon LC and Vreugdenhil D (eds) Progress in Plant Growth Regulation, pp 555–565. Dordrecht: Kluwer

    Google Scholar 

  8. King RW, Evans LT, Mander LN and Pharis RP (1993) Gibberellin structure and florigenic activity in Lolium temulentum long day plant. In: Abstracts, Frontiers of Gibberellin Research, RIKEN, Tokyo, 5–7 Sept., p 33

    Google Scholar 

  9. King RW and Pharis RP (1993) Searching for florigen(s): Gibberellins and other growth regulators. In: Abstracts, XV International Botanical Congress, Tokyo, 28 Aug–3 Sept

  10. Koshioka M, Harada J, Takeno K, Noma M, Sassa T, Ogiyama K, Taylor JS, Rood SB, Legge RL and Pharis RP (1983) Reversed-phase C18 high-performance liquid chromatography of acidic and conjugated gibberellins. J Chromatogr 256: 101–115

    Article  Google Scholar 

  11. Koshioka M, Takeno K, Beall FD and Pharis RP (1983) Purification and separation of plant gibberellins from their precursors and glucosyl conjugates. Plant Physiol 73: 398–406

    Google Scholar 

  12. Lenton JR, Hedden P and Gale MD (1987) Gibberellin insensitivity and depletion in wheat — consequences for development. In: Hoad GV, Lenton JR, Jackson MB and Aitken RK (eds) Hormone Action in Plant Development — A Critical Appraisal, pp 145–160. London: Butterworths

    Google Scholar 

  13. Murofushi N, Durley RC and Pharis RP (1977) Preparation of radioactive gibberellins A20, A5 and A8. Agric Biol Chem 41: 1075–1079

    Google Scholar 

  14. Nakayama I, Kamiya Y, Kobayashi M, Abe H and Sakurai A (1990) Effects of a plant growth regulator, prohexadione, on the biosynthesis of gibberellins in cell free systems derived from immature seeds. Plant Cell Physiol 31: 1183–1190

    Google Scholar 

  15. Nakayama I, Miyazawa T, Kobayashi M, Kamiya Y, Abe H and Sakurai A (1990) Effects of a new plant growth regulator prohexadione calcium (BX-112) on shoot elongation caused by exogenously applied gibberellins in rice (Oryza sativa L.) seedlings. Plant Cell Physiol 31: 195–200

    Google Scholar 

  16. Nakayama I, Miyazawa T, Kobayashi M, Kamiya Y, Abe H and Sakurai A (1991) Studies on the action of the plant growth regulators BX-112, DOCHC, and DOCHC-Et. In: Takahashi N, Phinney BO and MacMillan J (eds) Gibberellins, pp 311–319. New York: Springer-Verlag

    Google Scholar 

  17. Nishijima T and Katsura N (1989) A modified microdrop bioassay using dwarf rice for detection of femtomol quantities of gibberellins. Plant Cell Physiol 30: 623–627

    Google Scholar 

  18. Pharis RP, Mandel RM, Pearce DW, Takagi M, Foster KR, Mander LN, Evans LT, King RW, Taylor JS, Harker RN and Jackson DI (1993) Research on the C-16,17-dihydro gibberellin class of hormones with emphasis on physiology and possible practical uses. In: Abstracts, Recent Progress in the Research on the Plant Hormones and Related Substances, Tokyo, 4 Sept., pp 17–18

  19. Phinney BO (1985) Gibberellin A1, dwarfism and shoot elongation in higher plants. Biol Plant 27: 47–53

    Google Scholar 

  20. Rademacher W (1991) Inhibitors of gibberellin biosynthesis: Application in agriculture and horticulture. In: Takahashi N, Phinney BO and MacMillan J (eds) Gibberellins, pp 296–310. New York: Springer-Verlag

    Google Scholar 

  21. Ross JJ, Reid JB, Gaskin P and MacMillan J (1989) Internode length in Pisum. Estimation of GA1 levels in genotypes Le, le and le d. Physiol Plant 76: 173–176

    Google Scholar 

  22. Smith VA, Albone KS and MacMillan J (1991) Enzymatic 3β-hydroxylation of gibberellins A20 and A5. In: Takahashi N, Phinney BO and MacMillan J (eds) Gibberellins, pp 62–71. New York: Springer-Verlag

    Google Scholar 

  23. Willis CL, Gaskin P and MacMillan J (1988) [1β,2β,3β-3H3]Gibberellin A20: confirmation of structure by 3H NMR and by mass spectrometry. Phytochemistry 27: 3970–3972

    Article  Google Scholar 

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Author notes

  1. Makiko Takagi

    Present address: Department of Food Sciences, Shizuoka Eiwa College, 422, Shizuoka, Japan

Authors and Affiliations

  1. Plant Physiology Research Group, Department of Biological Sciences, University of Calgary, T2N 1N4, Calgary, Alberta, Canada

    Makiko Takagi, David W. Pearce, Loeke M. Janzen & Richard P. Pharis

Authors
  1. Makiko Takagi
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  2. David W. Pearce
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  3. Loeke M. Janzen
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  4. Richard P. Pharis
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Takagi, M., Pearce, D.W., Janzen, L.M. et al. Effect of exo-16,17-dihydro-gibberellin A5 on gibberellin A20 metabolism in seedlings of dwarf rice (Oryza sativa L. cv. Tan-ginbozu). Plant Growth Regul 15, 207–213 (1994). https://doi.org/10.1007/BF00029893

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