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The 5′ flanking region of a barley B hordein gene controls tissue and developmental specific CAT expression in tobacco plants

Plant Molecular Biology volume 10, pages 359–366 (1988)Cite this article

Abstract

The 549 base pairs of the 5′ flanking region of a barley seed storage protein (B1 hordein) gene were linked to the reporter gene encoding chloramphenicol acetyl transferase (CAT). The chimaeric gene was transferred into tobacco plants using Agrobacterium tumefaciens. CAT enzyme activity was detected in the seeds, but not in the leaves, of the transgenic plants. Furthermore, enzyme activity was found only in the endosperm, and only from fifteen days after pollination. In contrast, the constitutive 19S promoter from cauliflower mosaic virus (CaMV) directed the expression of the CAT gene in the leaves as well as in both the endosperm and embryo and at all stages in seed development.

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Affiliations

  1. AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts, UK

    Claire Marris, Patrick Gallois, Jane Copley & Martin Kreis

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  1. Claire Marris
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  2. Patrick Gallois
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  3. Jane Copley
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  4. Martin Kreis
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Marris, C., Gallois, P., Copley, J. et al. The 5′ flanking region of a barley B hordein gene controls tissue and developmental specific CAT expression in tobacco plants. Plant Mol Biol 10, 359–366 (1988). https://doi.org/10.1007/BF00029886

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  • DOI: https://doi.org/10.1007/BF00029886

Key words

  • barley
  • tissue-specific gene expression
  • endosperm
  • hordeins
  • tobacco