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Plant Molecular Biology

, Volume 10, Issue 4, pp 359–366 | Cite as

The 5′ flanking region of a barley B hordein gene controls tissue and developmental specific CAT expression in tobacco plants

  • Claire Marris
  • Patrick Gallois
  • Jane Copley
  • Martin Kreis
Article

Abstract

The 549 base pairs of the 5′ flanking region of a barley seed storage protein (B1 hordein) gene were linked to the reporter gene encoding chloramphenicol acetyl transferase (CAT). The chimaeric gene was transferred into tobacco plants using Agrobacterium tumefaciens. CAT enzyme activity was detected in the seeds, but not in the leaves, of the transgenic plants. Furthermore, enzyme activity was found only in the endosperm, and only from fifteen days after pollination. In contrast, the constitutive 19S promoter from cauliflower mosaic virus (CaMV) directed the expression of the CAT gene in the leaves as well as in both the endosperm and embryo and at all stages in seed development.

Key words

barley tissue-specific gene expression endosperm hordeins tobacco 

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Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • Claire Marris
    • 1
  • Patrick Gallois
    • 1
  • Jane Copley
    • 1
  • Martin Kreis
    • 1
  1. 1.AFRC Institute of Arable Crops ResearchRothamsted Experimental StationHarpendenUK

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