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Total recovery of O2 evolution and nanosecond reduction kinetics of chlorophyll-a +II (P-680+) after inhibition of water cleavage with acetate

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Abstract

Oxygen evolution and reduction kinetics of the photooxidized Chl-aII + have been measured in oxygen-evolving complexes from the thermophilic cyanobacterium Synechococcus sp.

  1. 1.

    Incubation of PS II particles with acetate resulted in an inhibition of oxygen evolution and a retardation of the Chl-aII +=reduction kinetics from the nanosecond range to the microsecond range, indicating a modification of the donor side of photosystem II (PS II).

  2. 2.

    After the first two flashes given to a dark-adapted, acetate treated sample, Chl-aII + was re-reduced with a half-life time of 160 μs by a component of the donor side of PS II. Under repetitive excitation Chl-aII + was re-reduced in 500 μs by electron back reaction from the primary acceptor QA - (X-320-). Obviously, in the presence of acetate only two electrons are available from the donor side.

  3. 3.

    Both oxygen evolution and nanosecond reduction kinetics of Chl-aII + were restored to the control level when acetate was removed.

  4. 4.

    The results indicate a tight coupling between O2 evolution and nanosecond reduction kinetics of Chl-aII +.

  5. 5.

    The reversible inhibition is probably due to a replacement of Cl- by acetate within the water splitting enzyme.

  6. 6.

    Due to its strongly retarded kinetics, the reversibly modified system may facilitate investigations of the mechanism of the donor side.

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Abbreviations

Chl:

chlorophyll

PpBQ:

phenyl-p-benzoquinone

PS:

photosystem

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Authors and Affiliations

  1. Max-Volmer-Institut für Biophysikalische und Physikalische Chemie, Technische Universität Berlin, Strasse des 17. Juni 135, 1000, Berlin 12, BRD

    Ö Saygin, S. Gerken, B. Mever & H. T. Witt

Authors
  1. Ö Saygin
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  2. S. Gerken
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  3. B. Mever
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  4. H. T. Witt
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Additional information

This work is dedicated to Prof. Dr. L.N.M. Duysens on the occasion of his retirement.

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Saygin, Ö., Gerken, S., Mever, B. et al. Total recovery of O2 evolution and nanosecond reduction kinetics of chlorophyll-a +II (P-680+) after inhibition of water cleavage with acetate. Photosynth Res 9, 71–78 (1986). https://doi.org/10.1007/BF00029733

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  • DOI: https://doi.org/10.1007/BF00029733

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