Abstract
Shoot multiplication of Gentiana kurroo Royle, a threatened medicinal plant species, was achieved in vitro using shoot tips and nodal segments as explants. Fifteen-fold shoot multiplication occurred every 6 weeks on Murashige and Skoog's medium (MS) containing 8.9 μM benzyladenine and 1.1 μM 1-naphthaleneacetic acid. Rooting was accomplished successfully in excised shoots grown on MS basal medium containing 6% sucrose.
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Abbreviations
- BA:
-
6-benzyladenine
- IAA:
-
Indole-3-acetic acid
- IBA:
-
Indole-3-butyric acid
- MS:
-
Murashige and Skoog's medium
- NAA:
-
1-naphthaleneacetic acid
References
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Sharma, N., Chandel, K.P.S. & Paul, A. In vitro propagation of Gentiana kurroo — an indigenous threatened plant of medicinal importance. Plant Cell Tiss Organ Cult 34, 307–309 (1993). https://doi.org/10.1007/BF00029722
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DOI: https://doi.org/10.1007/BF00029722