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Plant regeneration from hypocotyl segments of Lupinus luteus cv. L. Aurea

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Abstract

Complete plants of Lupinus luteus L. cv. Aurea that were regenerated from hypocotyl segments, bloomed, produced seeds and were efficiently nodulated by Bradyrhizobium sp. strains. The highest rates of shoot formation were obtained on A medium plus 1.3% agar with 10.0 μM 2-isopentenyladenine (2iP) and 0.11 μM naphthaleneacetic acid (NAA); the best rooting was achieved on a medium with 0.5 μM NAA plus 0.05 μM 2iP. Afterward, plantlets were transferred to either perlite or peat-containing pots and irrigated with a N-free nutrient solution until maturity. Direct rooting of hypocotyls could also be obtained on A medium with 1% agar.

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Authors and Affiliations

  1. D.G.I.F.A.P., C.I.D.A. ‘Las Torres’, Alcalá del Río, Apdo. Oficial, 41200, Sevilla, Spain

    Antonio Daza & Manuel A. Chamber

Authors
  1. Antonio Daza
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  2. Manuel A. Chamber
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Daza, A., Chamber, M.A. Plant regeneration from hypocotyl segments of Lupinus luteus cv. L. Aurea. Plant Cell Tiss Organ Cult 34, 303–305 (1993). https://doi.org/10.1007/BF00029721

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  • DOI: https://doi.org/10.1007/BF00029721

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