Abstract
The in vitro embryogenic response of nine varieties of alfalfa (Medicago sativa L.) grown in México (five Mexican varieties: Puebla 76, Inia 76, Bajío 76, Sintético I and Sintético II and four foreign or introduced varieties: Moapa 69, San Joaquín II, Hairy Peruvian and Valenciana) were tested. We screened 25 genotypes from each variety in four tissue culture protocols. All the varieties, except San Joaquín II, gave a positive response in one or more of the protocols tested. The response in each variety was low; this was also observed in a wider screening performed with the varieties Moapa 69, Hairy Peruvian, Sintético I and Sintético II. Two plants from Moapa 69 were regenerated and appeared normal.
This is a preview of subscription content, log in via an institution to check access.
References
Blaydes DF (1966) Interaction of kinetin and various inhibitors in the growth of soybean tissue. Physiol. Plant. 19: 748–753
Brown DCW & Atanassov A (1985) Role of genetic background in somatic embryogenesis in Medicago. Plant Cell Tiss. Org. Cult. 4: 111–222
dos Santos AVP, Cutter EG & Davey MR (1983) Origin and development of somatic embryos in Medicago sativa L. (Alfalfa). Protoplasma 117: 107–115
Lupotto E (1983) Propagation of an embryogenic culture of Medicago sativa L.. Z. Pflanzenphysiol. 111: 95–104
Meijer EGM & Brown DCW (1987) A novel system for somatic embryogenesis in Medicago sativa. Physiol. Plant. 69: 591–596
Mitten DH, Sato SJ & Skokut TA (1984) In vitro regenerative potential of alfalfa germplasm sources. Crop Sci. 24: 943–945
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Nagarajan P, McKenzie JS & Walton PD (1986) Embryogenesis and plant regeneration of Medicago spp. in tissue culture. Plant Cell Rep. 5: 77–80
Novak FJ & Konecna D (1982) Somatic embryogenesis in callus and cell suspension cultures of alfalfa (Medicago sativa L.) Z. Pflanzenphysiol. 105: 279–284
Saunders JW & Bingham ET (1975) Growth regulator effects on bud initiation in callus cultures of Medicago sativa. Amer. J. Bot. 62: 850–855
Schenk RU & Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50: 199–204
Skokut TA, Manchester J & Schaefer J (1985) Regeneration of alfalfa tissue culture stimulation of somatic embryo production by amino acids and N-15 NMR determination of nitrogen utilization. Plant. Physiol. 79: 579–583
Stuart DA & Strickland SG (1984) Somatic embryogenesis cultures of Medicago sativa L. II The interaction of amino acids with ammonium. Plant Sci. Lett. 34: 175–181
Walker KA & Sato SJ (1981) Morphogenesis in callus tissue of Medicago sativa: the role of ammonium ion in somatic embryogenesis. Plant Cell Tiss. Org. Cult. 1: 109–121
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fuentes, S.I., Suárez, R., Villegas, T. et al. Embryogenic response of Mexican alfalfa (Medicago sativa) varieties. Plant Cell Tiss Organ Cult 34, 299–302 (1993). https://doi.org/10.1007/BF00029720
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00029720