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In vitro regeneration through organogenesis of Meconopsis simplicifolia — An endangered ornamental species

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Abstract

Meconopsis simplicifolia (D.Don) Walp. could be propagated by induction of adventitious shoots from callus produced on hypocotyl, cotyledon and rosette leaf explants of 4-month-old seedlings. Callus was initiated on agar-solidified Murashige and Skoog medium supplemented with 1 μM kinetin +10 μM α-naphthaleneacetic acid (NAA). Shoots formed when the callus was subcultured on medium supplemented with kinetin or benzyladenine (BA) in combination with NAA, indoleacetic acid, indolebutyric acid or gibberellic acid. Excised shoots were rooted on medium containing auxin with 10 μM NAA producing the best rooting (55%).

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Abbreviations

BA:

6-benzyladenine

2,4-d :

2,4-dichlorophenoxy-acetic acid

FAA:

formalin-acetic acid-alcohol

GA3 :

gibberellic acid

IAA:

indole-3-acetic acid

IBA:

β-indolebutyric acid

NAA:

α-naphthaleneacetic acid

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Author notes

  1. I. M. Sulaiman

    Present address: Eukaryotic Gene Expression Laboratory, National Institute of Immunology, 110067, New Delhi, India

Authors and Affiliations

  1. Department of Botany, University of Delhi, 110007, Delhi, India

    I. M. Sulaiman & C. R. Babu

Authors
  1. I. M. Sulaiman
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  2. C. R. Babu
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Sulaiman, I.M., Babu, C.R. In vitro regeneration through organogenesis of Meconopsis simplicifolia — An endangered ornamental species. Plant Cell Tiss Organ Cult 34, 295–298 (1993). https://doi.org/10.1007/BF00029719

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  • DOI: https://doi.org/10.1007/BF00029719

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