Abstract
Synechocystis sp. PCC6803, a cyanobacterium, possesses an unusual gene (fusB) which encodes a protein with strong homology to protein synthesis elongation factor G (EF-G), although it is not linked to the classical str operon. The fusB gene is redundant, since a Synechocystis gene similar to str operon-encoded fusA genes of other bacteria is also present (based on PCR and hybridization results). There is no evidence for the presence of a fusB homologue in other bacteria. The Synechocystis fusB gene encodes unusual amino acids at some positions that are highly conserved in fusA genes of other prokaryotes.
References
Akkaya MS, Welcsh PL, Wolfe MA, Duerr BK, Becktel WJ, Breitenberger CA: Purification and N-terminal sequence analysis of pea chloroplast protein synthesis factor EF-G. Arch Biochem Biophys 308: 109–117 (1994).
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol 215: 403–410 (1990).
Anderson SL, McIntosh L: Partial conservation of the 5′ ndhE-psaC-ndhD 3′ gene arrangement of chloroplasts in the cyanobacterium Synechocystis sp. PCC 6803: implications for NDH-D function in cyanobacteria and chloroplasts. Plant Mol Biol 16: 487–499 (1991).
Baldauf SL, Manhart JR, Palmer JD: Different fates of the chloroplast tufA gene following its transfer to the nucleus in green algae. Proc Natl Acad Sci USA 87: 5317–5321 (1990).
Buttarelli FR, Calogero RA, Gualerzi CO, Pon CL: Characterization of the str operon genes from Spirulina platensis and their evolutionary relationship to those of other prokaryotes. Mol Gen Genet 217: 97–104 (1989).
Cammarano P, Palm P, Creti R, Ceccarelli E, Sanangelantoni AM, Tiboni O. Early evolutionary relationships among known life form inferred from elongation factor EF-2/EF-G sequences: Phylogenetic coherence and structure of the archaeal domain. J Mol Evol 34: 396–405 (1992).
Douglas SE: Unusual organization of a ribosomal protein operon in the plastid genome of Cryptomonas Ф: evolutionary considerations. Curr Genet 19: 289–294 (1991).
Ellersiek U, Steinmüller K: Cloning and transcription analysis of the ndh(A-I-G-E) gene cluster and the ndhD gene of the cyanobacterium Synechocystis sp. PCC6803. Plant Mol Biol 20: 1097–1110 (1992).
Filer D, Furano AV: Duplication of the tuf gene, which encodes peptide chain elongation factor Tu, is widespread in Gram-negative bacteria. J Bact 48: 1006–1011 (1981).
Giovannoni SJ, Turner S, Olsen GJ, Barns S, Lane DJ, Pace NR: Evolutionary relationships among cyanobacteria and green chloroplasts. J Bact 170: 3584–3592 (1988).
Herdman M, Janvier M, Rippka R, Stanier RY: Genome size of cyanobacteria. J Gen Microbiol 111: 73–85 (1979).
Mayes SR, Cook KM, Barber J: Nucleotide sequence of the second psbG gene in Synechocystis 6803. Possible implications for psbG function as a NAD(P)H dehydrogenase subunit gene. FEBS Lett 262: 49–54 (1990).
Meng BY, Shinozaki K, Sugiura M: Genes for the ribosomal proteins S12 and S7 and elongation factors EF-G and EF-Tu of the cyanobacterium, Anacystis nidulans: structural homology between 16S rRNA and S7 mRNA. Mol Gen Genet 216: 25–30 (1989).
Mickel FS, Spremulli LL: Organization of the genes for protein synthesis elongation factors Tu and G in the cyanobacterium Anacystis nidulans. J Bact 166: 78–82 (1986).
Steinmüller K: Identification of a second psaC gene in the cyanobacterium Synechocystis sp. PCC6803. Plant Mol Biol 20: 997–1001 (1992).
Steinmüller K, Ley AC, Steinmetz AA, Sayre RT, Bogorad L: Characterization of the ndhC-psbG-ORF157/159 operon of maize plastid DNA and of the cyanobacterium Synechocystis sp. PCC6803. Mol Gen Genet 216: 60–69 (1989).
Tiboni O, Di Pasquale G: Organization of genes for ribosomal proteins S7 and S12, elongation factors EF-Tu and EF-G in the cyanobacterium Spirulina platensis. Biochim Biophys Acta 908: 113–122 (1987).
Tiboni O, Cantoni R, Creti R, Cammarano P, Sanangelantoni AM: Phylogenetic depth of Thermotoga maritima inferred from analysis of the fus gene: Amino acid sequence of elongation factor G and organization of the Thermotoga str operon. J Mol Evol 33: 142–151 (1991).
Torres JH, Breitenberger CA, Spielmann A, Stutz B: Cloning and sequencing of a soybean nuclear gene coding for a chloroplast translation elongation factor EF-G. Biochim Biophys Acta 1174: 191–194 (1993).
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Welcsh, P.I., Johnson, D.R., Zhang, Y. et al. Synechocystis sp. PCC6803 fusB gene, located outside of the str operon, encodes a polypeptide related to protein synthesis factor EF-G. Plant Mol Biol 25, 735–738 (1994). https://doi.org/10.1007/BF00029610
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DOI: https://doi.org/10.1007/BF00029610