Abstract
The flash-induced kinetics of various characteristics of Photosystem II (PS II) in the thylakoids of oxygenic plants are modulated by a period of two, due to the function of a two-electron gate in the electron acceptor side, and by a period of four, due to the changes in the state of the oxygen-evolving complex. In the absence of inhibitors of PS II, the assignment of measured signal to the oxygen-evolving complex or to quinone acceptor side has frequently been done on the basis of the periodicity of its flash-induced oscillations, i.e. four or two. However, in some circumstances, the period four oscillatory processes of the donor side of PS II can generate period two oscillations. It is shown here that in the Kok model of oxygen evolution (equal misses and equal double hits), the sum of the concentrations of the S 0 and S 2 states (as well as the sum of concentrations of S 1 and S 3 states) oscillates with period of two: S 0+S 2→S 1+S 3→S 0+S 2→S 1+S 3. Moreover, in the generalized Kok model (with specific miss factors and double hits for each S-state) there always exist such ε0, ε1, ε2, ε3 that the sum ε0[S0] + ε1[S1] + ε2[S2] + ε3[S3] oscillates with period of two as a function of flash number. Any other coefficients which are linearly connected with these coefficients, % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGak0dh9WrFfpC0xh9vqqj-hEeeu0xXdbba9frFj0-OqFf% ea0dXdd9vqaq-JfrVkFHe9pgea0dXdar-Jb9hs0dXdbPYxe9vr0-vr% 0-vqpWqaaeaabiGaciaacaqabeaadaqaaqaaaOqaaiqbew7aLzaaja% aaaa!3917!\[\hat \varepsilon \]i = c1εi + c2, also generate binary oscillations of this sum. Therefore, the decomposition of the flash-induced oscillations of some measured parameters into binary oscillations, depending only on the acceptor side of PS II, and quaternary oscillations, depending only on the donor side of PS II, becomes practically impossible when measured with techniques (such as fluorescence of chlorophyll a, delayed fluorescence, electrochromic shift, transmembrane electrical potential, changes of pH and others) that could not spectrally distinguish the donor and acceptor sides. This property of the Kok cycle puts limits on the simultaneous analysis of the donor and acceptor sides of the RC of PS II in vivo and suggests that binary oscillations are no longer a certain indicator of the origin of a signal in the acceptor side of PS II.
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Abbreviations
- PS II:
-
Photosystem II
- P680:
-
primary electron donor of reaction center of PS II
- QA :
-
one electron acceptor plastoquinone
- QB :
-
two electron acceptor plastoquinone
- S n :
-
redox state of the oxygen evolving complex, where n=0,1,2,3 and 4
- Chl a :
-
chlorophyll a
References
Ananyev G, Wydrzynski T, Renger G and Klimov V (1992) Transient peroxide formation by the manganese-containing, redox-active donor side of Photosystem II upon inhibition of O2 evolution with lauroylcholine chloride. Biochim Biophys Acta 1100: 303–311
Beckwith AC and Jursinic PA (1982) An alternative mathematical approach to the analysis of photosynthetic oxygen evolution. J Theor Biol 97: 251–265
Bouges-Bocquet B (1973) Electron transfer between the two photosystems in spinach chloroplasts. Biochim Biophys Acta 314: 250–256
Bowes JM and Crofts AR (1980) Binary oscillations in the rate of reoxidation of the primary acceptor of Photosystem II. Biochim Biophys Acta 590: 373–384
Crofts AR and Wraight CA (1983) The electrochemical domain of photosynthesis. Biochim Biophys Acta 726: 149–185
Delosme R (1971) New results about chlorophyll fluorescence ‘in vivo’. In: Forti G, Avron M and Melandri A (eds) Proc of the II Int Congress on Photosynth Res, Vol I, pp 187–195. Dr W Junk NV Publishers, The Hague
Delrieu MJ (1974) Simple explanation of the misses in the cooperation of charges in photosynthetic O2 evolution. Photochem Photobiol 20: 441–454
Delrieu MJ (1983) Evidence for unequal misses in oxygen flash yield sequence in photosynthesis. Z Naturforsh 38c: 247–258
Eaton-Rye JJ and Govindjee (1988) Electron transfer through the quinone acceptor complex of Photosystem II in bicarbonate-depleted spinach thylakoid membranes as a function of actinic flash number and frequency. Biochim Biophys Acta 935: 237–247
Govindjee (1995) Sixty three years since Kautsky: Chlorophyll a fluorescence. Aust J Plant Physiol 22: 131–160
Hsu B-D (1993) Evidence for the contribution of the S-state transitions of oxygen evolution to the initial phase of fluorescence induction. Photosynth Res 36: 81–88
Joliot P and Joliot A (1971) Studies on the quenching properties of Photosystem II electron acceptor. In: Forti G, Avron M and Melandri A (eds) Proc of the II Int Congress on Photosynth Res, Vol I, pp 26–38. Dr W Junk NV Publishers, The Hague
Joliot P and Kok B (1975) Oxygen evolution in photosynthesis. In: Govindjee (ed) Bioenergetics of Photosynthesis, pp 388–413. Academic Press, New York
Joliot P, Barbieri G and Chabaud R (1969) Un nouveau modèle des centers photochimiques du système II. Photochem Photobiol 10: 309–329
Klimov V, Ananyev G, Zastryzhnaya O, Wydrzynski T and Renger G (1993) Photoproduction of hydrogen peroxide in Photosystem II membrane fragments — a comparison of four signals. Photosynth Res 38: 409–416
Kok B, Forbush B and McGloin M (1970) Cooperation of charges in photosynthetic O2 evolution — I. A linear four step mechanism. Photochem Photobiol 11: 467–475
Kramer DM, Robinson HH and Crofts AR (1990) A portable multiflash kinetic fluorimeter for measurement of donor and acceptor reactions of Photosystem 2 in leaves and intact plants under field conditions. Photosynth Res 26: 181–193
Mar T and Govindjee (1972) Kinetic models of oxygen evolution in photosynthesis. J Theor Biol 36: 427–446
Meunier PC (1993) Oxygen evolution by Photosystem II: The contribution of backward transitions to the anomalous behavior of double-hits revealed by a new analysis method. Photosynth Res 36: 111–118
Shinkarev VP and Wraight CA (1993a) Oxygen evolution in photosynthesis. From unicycle to bicycle. Proc Natl Acad Sci USA 90: 1834–1838
Shinkarev VP and Wraight CA (1993b) Kinetic factors in the bicycle model of oxygen evolution by Photosystem II. Photosynth Res 38: 315–321
Shinkarev VP, Verkhovsky MI, Kaurov BS and Rubin AB (1981) Kinetic model of the function of two-electron gate in the photosynthetic reaction center of purple bacteria. Mol Biol (USSR) 15: 1069–1082
Velthuys BR and Amesz J (1974) Charge accumulation at the reducing side of system 2 of photosynthesis. Biochim Biophys Acta 333: 85–94
Vos MH, van Gorkom HJ and van Leeuwen PJ (1991) An electroluminescence study of stabilization reactions in the oxygen-evolving complex of Photosystem II. Biochim Biophys Acta 1056: 27–39
Weiss C and Sauer K (1970) Activation kinetics of photosynthetic oxygen evolution under 20–40 nanosecond laser flashes. Photochem Photobiol 11: 495–501
Zankel KL (1973) Rapid fluorescence changes observed in chloroplasts, their relationship to the O2 evolving system. Biochim Biophys Acta 325: 138–148
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This paper is dedicated to the memory of Alexander Kononenko.
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Shinkarev, V.P. Binary oscillations in the Kok model of oxygen evolution in oxygenic photosynthesis. Photosynth Res 48, 411–417 (1996). https://doi.org/10.1007/BF00029473
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DOI: https://doi.org/10.1007/BF00029473