Abstract
Various container systems have been described in which soil regions available to hyphae only are separated from the mycorrhizal root region by 30–60 μm mesh screens to study nutrient exchange between plants and fungi in the mycorrhizal symbiosis. The screens designed up to now prevent penetration by roots but allow easy passage of fungal hyphae as well as diffusion or mass flow of water and nutrient solutions. We tested hydrophobic microporous polytetrafluorethylene (PTFE) membranes (GORE-TEXR) with 5 to 15 μm diameter pores in an attempt to obtain a better seal between compartments and to prevent uncontrolled nutrient transport by diffusion or mass flow. We found that these membranes completely prevented diffusion or mass flow of ions between two soil compartments but could be penetrated easily by the vesicular-arbuscular mycorrhizal fungus Glomus mosseae, as demonstrated by the rapid colonization of soybean roots (Glycine max L.) from an inoculum across the membranes.
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References
Allan A 1982 PTFE membrane laminates — the filter media of the future. Chem. Eng. (London) 385, 374–376.
Bethlenfalvay G J, Reyes-Solis M G, Camel S B and Ferrera-Cerrato R 1991 Nutrient transfer between the root zones of soybean and maize plants connected by a common mycorrhizal mycelium. Physiol. Plant. 82, 423–432.
Faber B A, Zasoski R J, Munns D N and Shackel K 1991 A method for measuring hyphal nutrient and water uptake in mycorrhizal plants. Can. J. Bot. 69, 87–94.
George E, Häussler K-U, Vetterlein D, Gorgus E and Marschner H 1992 Water and nutrient translocation by hyphae of Glomus mosseae. Can. J. Bot. 70, 2130–2137.
Gerdemann J W and Trappe J M 1974 The Endogonaceae in the Pacific Northwest. Mycologia Memoir 5, 76 p.
Giovannetti M and Mosse B 1980 An evaluation of techniques for measuring vesicular-arbuscular mycorrhizal infection in roots. New Phytol. 84, 489–500.
Haystead A, Malajczuk N and Grove T S 1988 Underground transfer of nitrogen between pasture plants infected with vesicular-arbuscular mycorrhizal fungi. New Phytol. 108, 417–423.
Jakobsen I, Abbott L K and Robson A D 1992 External hyphae of vesicular-arbuscular mycorrhizal fungi associated with Trifolium subterraneum L. 1. Spread of hyphae and phosphorus inflow into roots. New Phytol. 120, 371–380.
Koide R T and Schreiner R P 1992 Regulation of the vesicular-arbuscular mycorrhizal symbiosis. Annu. Rev. Plant Physiol. Plant Mol. Biol. 43, 557–581.
Li X-L, George E and Marschner H 1991 Phosphorus depletion and pH decrease at the root-soil and hyphae-soil interfaces of VA mycorrhizal white clover fertilized with ammonium. New Phytol. 119, 397–404.
Nicolson T H 1959 Mycorrhiza in the graminea. I. Vesicular-arbuscular endophytes, with special reference to the external phase. Trans. Br. Mycol. Soc. 42, 421–438.
Phillips J M and Hayman D S 1970 Improved procedure for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Trans. Br. Myc. Soc. 55, 158–161.
Schneider K and van Gassel T J 1984. Membrandestillation. Chem.-Ing.-Tech. 56, 514–521.
Schüepp H, Miller D D and Bodmer M 1987 A new technique for monitoring hyphal growth of vesicular-arbuscular mycorrhizal fungi through soil. Trans. Br. Myc. Soc. 89, 429–435.
Strathmann H and Chmiel H 1985 Membranen in der Verfahrenstechnik. Chem.-Ing.-Tech. 57, 581–596.
Van Kessel C, Singleton P W and Hoben H J 1985 Enhanced N-transfer from soybean to maize by vesicular-arbuscular mycorrhizal (VAM) fungi. Plant Physiol. 79, 562–563.
Wyss P, Boller T and Wiemken A 1991 Phytoalexin response is elicited by a pathogen (Rhizoctonia solani) but not by a mycorrhizal fungus (Glomus mosseae) in soybean roots. Experientia 47, 395–399.
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Mäder, P., Vierheilig, H., Alt, M. et al. Boundaries between soil compartments formed by microporous hydrophobic membranes (GORE-TEXR) can be crossed by vesicular-arbuscular mycorrizal fungi but not by ions in the soil solution. Plant Soil 152, 201–206 (1993). https://doi.org/10.1007/BF00029089
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DOI: https://doi.org/10.1007/BF00029089