Abstract
Antibodies raised against yeast heat shock protein (HSP) 104 recognized a heat-inducible polypeptide with a molecular mass of 110 kDa in shoot tissue of young rice seedlings. Root tissue of the same age showed no immuno-reaction with yeast HSP 104 antibodies. The 110 kDa polypeptide of rice was also shown to be abscisic acid-inducible in young seedlings. Though this polypeptide was seen to be constitutively present in the flag leaf of 90-day-old field-grown plant, it was not much affected by either heat shock or abscisic acid in this case.
References
Bradford M: A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254 (1976).
Bork P, Sander C, Valencia A: An ATPase domain common to prokaryotic cell cycle proteins, sugar kinases, actin and HSP 70 heat shock proteins. Proc Natl Acad Sci USA 89: 7290–7294 (1992).
Czarnecka E, Edelman L, Key JL: Comparative analysis of physical stress responses in soybean seedlings using cloned heat shock cDNAs. Plant Mol Biol 3: 45–58 (1984).
Damerval C, Guillox ML, Blaisonneau J, de Vienne D: A simplification of Heukeshoven and Dernick's silver staining of proteins. Electrophoresis 8: 158–159 (1987).
Gottesman S, Squires C, Pichersky E, Carrington M, Hobbs M, Mattick JS, Dalrymple B, Kuramitsu H, Shiroza T, Foster T, Clark WP, Ross B, Squires CL, Maurizi MR: Conservation of the regulatory subunit for the Clp ATP-dependent protease in prokaryotes and eukaryotes. Proc Natl Acad Sci USA 87: 3513–3517 (1990).
Heikkila JJ, Papp JET, Schultz GA, Bewley JD: Induction of heat shock protein messenger RNA in maize mesocotyls by water stress, abscisic acid, and wounding. Plant Physiol 76: 270–274 (1984).
Hendershot KL, Weng J, Nguyen HT: Induction temperature of heat shock protein synthesis in wheat. Crop Sci 32: 256–261 (1992).
Hong B, Barg R, Ho T-h D: Developmental and organ-specific expression of an ABA- and stress-induced protein in barley. Plant Mol Biol 18: 663–674 (1992).
Houde M, Dhindsa RS, Sarhan F: A molecular marker to select for freezing tolerance in Gramineae. Mol Gen Genet 234: 43–48 (1992).
Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685 (1979).
Lindquist S: The heat shock response. Annu Rev Biochem 55: 1151–1191 (1986).
Parsell DA, Sanchez Y, Stitzel JD, Lindquist S: Hsp 104 is a highly conserved protein with two essential nucleotide-binding sites. Nature 353: 270–273 (1991).
Sanchez Y, Lindquist S: HSP 104 required for induced thermotolerance. Science 248: 1112–1115 (1990).
Sanchez Y, Taulien J, Borkovich KA, Lindquist S: HSP 104 is required for tolerance to many forms of stress. EMBO J 11: 2357–2364 (1992).
Towbin H, Staehelin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350–4354 (1979).
Yaffe MP, Schatz G: Two nuclear mutations that block mitochondrial protein import in yeast. Proc Natl Acad Sci USA 81: 4819–4823 (1984).
Zimmerman FK, Kern H, Rosenberger H: A yeast strain for simultaneous detection of induced mitotic crossing over, mitotic gene conversion and reverse mutation. Mutation Res 28: 381–388 (1975).
Zivy M, Thiellement H, de Vienne D, Hofmann JP: Study on nuclear and cytoplasmic genome expression in wheat by two-dimensional gel electrophoresis. Theor Appl Genet 66: 1–7 (1983).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Singla, S.L., Grover, A. Antibodies raised against yeast HSP 104 cross-react with a heat-and abscisic acid-regulated polypeptide in rice. Plant Mol Biol 22, 1177–1180 (1993). https://doi.org/10.1007/BF00028989
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00028989