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Isolation and characterization of a genomic sequence encoding the maizeCat3 catalase gene

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Abstract

A maize genomic DNA library (Zea mays L. W64A) was constructed using the lambda GEM-11 vector. A gene-specificCat3 cDNA probe was used to screen the library by plaque hybridization. Two independent clones, λ24C and gl27C, which hybridized to theCat3 cDNA probe, were isolated. Restriction maps were created for the two clones (λ24C and λ27C) encoding the maizeCat3 catalase gene. ASal I restriction fragment from each clone was subcloned into pBluescript KS+. Subsequent subcloning steps were performed to obtain plasmids suitable for sequencing. Both strands of the λ24C clone were sequenced.

Computer analysis of the putativeCat3 promoter region has not revealed any known transcription factor binding motifs. Analysis of the deduced C-terminal amino acids encoded byCat3 shows that CAT-3 lacks the SRL peroxisomal targeting sequence contained in the CAT-1 and CAT-2 isozymes. The DNA sequence and physical structure of theCat3 gene presented here will be used in characterizingcis- andtrans-acting factors affectingCat3 gene expression.

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Author notes

  1. Michael L. Abler

    Present address: MSU-DOE Plant Research Laboratory, Michigan State University, 48824-1312, East Lansing, MI, USA

Authors and Affiliations

  1. Department of Genetics, North Carolina State University, Box 7614, 27695-7614, Raleigh, NC, USA

    Michael L. Abler & John G. Scandalios

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  1. Michael L. Abler
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  2. John G. Scandalios
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Abler, M.L., Scandalios, J.G. Isolation and characterization of a genomic sequence encoding the maizeCat3 catalase gene. Plant Mol Biol 22, 1031–1038 (1993). https://doi.org/10.1007/BF00028975

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  • DOI: https://doi.org/10.1007/BF00028975

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