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Field survival of containerized red and jack pine seedlings inoculated with mycelial slurries of ectomycorrhizal fungi

Abstract

Red pine and jack pine seedlings growing in styroblocks were inoculated 8 wk after sowing with mycelium/agar slurries of 3 mycorrhizal fungi (Laccaria bicolor, Scleroderma citrinum, and an unidentified basidiomycete), and one suspected mycorrhizal fungus (Cantharellula umbonata). Seedlings inoculated with L. bicolor developed mycorrhizae earlier and in greater numbers than the other inoculation treatments, with red pine out-performing jack pine in both respects. At 34 wk following sowing, seedlings were outplanted on a cleared xeric site in Baraga Co., in Michigan's Upper Peninsula. Seedlings inoculated with C. umbonata failed to form mycorrhizae and were not outplanted. Inoculation treatments did not affect shoot or root weight at outplanting. Red pine inoculated with L. bicolor averaged 21% and 19% greater survival compared with control seedlings after one and two years in the field, respectively. Other inoculation treatments failed to increase seedling survival for either tree species. Jack pine demonstrated higher overall survival than did red pine for both years in all corresponding inoculation treatments.

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References

  • Anonymous. 1980. Interim Soil Survey Report of the Baraga County Area, Michigan. Baraga Soil Survey Party, in cooperation with the Baraga Soil Conservation District.

  • Anonymous. 1986. Climatological Data Annual Summary, Michigan. N.O.A.A. Vol. 101, No. 13. 36p.

  • Carlson, L.W. 1983. Guidelines for rearing containerized conifer seedlings in the Prairie Provinces (Revised). Environ. Can., Can. For. Serv., North. For. Res. Cent., Edmonton, Alberta. Inf. Rep. NOR-X-214E.

    Google Scholar 

  • Chakravarty, P. and Unestam, T. 1987a. Mycorrhizal fungi prevent disease in stressed pine seedlings. J. Phytopathol. 118: 335–340.

    Google Scholar 

  • Chakravarty, P. and Unestam, T. 1987b. Differential influence of ectomycorrhizae on plant growth and disease resistance in Pinus sylvestris seedlings. J. Phytopathol. 120: 104–120.

    Google Scholar 

  • Danielson, R.M., Visser, S. and Parkinson, J.D. 1984. The effectiveness of mycelial slurries of mycorrhizal fungi for inoculation of container-grown jack pine seedlings. Can. J. For. Res. 14: 140–142.

    Google Scholar 

  • Gagnon, J., Langlois, G.C. and Fortin, J.A. 1987. Growth of containerized jack pine seedlings inoculated with different ectomycorrhizal fungi under a controlled fertilization schedule. Can. J. For. Res. 17: 840–845.

    Google Scholar 

  • Harley, J.L. and Smith, S.E. 1983. Mycorrhizal Symbiosis. Academic Press, London. 483p.

    Google Scholar 

  • Jones, M.D. and Hutchinson, T.C. 1986. The effect of mycorrhizal infection on the response of Betula papyrifera to nickel and copper. New Phytol. 102: 429–442.

    Google Scholar 

  • Marx, D.H. 1969. The influence of ectotrophic mycorrhizal fungi on the resistance of pine roots to pathogenic infection. I. Antagonism of mycorrhizal fungi to root pathogenic fungi and soil bacteria. Phytopathol. 59: 153–163.

    Google Scholar 

  • Marx, D.H. 1976. Synthesis of ectomycorrhizae on loblolly pine seedlings with basidiospores of Pisolithus tinctorius. For. Sci. 22: 13–20.

    Google Scholar 

  • Michigan Department of Natural Resources. 1979. Michigan Forest Resources, an Assessment. State of Michigan, Lansing. 134 p.

    Google Scholar 

  • Molina, R. 1982. Use of the ectomycorrhizal fungus Laccaria laccata in forestry. I. Consistency between isolates in effective colonization of containerized conifer seedlings. Can. J. For. Res. 12: 469–473.

    Google Scholar 

  • Mueller, G.M. 1982. The genus Laccaria in North America excluding Mexico. Ph.D. Thesis, Univ. Tenn., Knoxville. 331 p.

  • Richter, D.L. and J.N.Bruhn. 1986. Pure culture synthesis of Pinus resinosa ectomycorrhizae with Scleroderma aurantium. Mycologia 78: 139–142.

    Google Scholar 

  • Sinclair, W.A., Sylvia, D.H. and Larson, A.O. 1982. Disease suppression and growth promotion in Douglas-fir seedlings by the ectomycorrhizal fungus Laccaria laccata. For. Sci. 28: 191–204.

    Google Scholar 

  • Tinus, R.W. and McDonald, S.E. 1979. How to Grow Tree Seedlings in Containers in Greenhouses. U.S.D.A. For. Serv. Gen. Tech. Rept. RM-60. 256 p.

  • Trappe, J.M. 1977. Selection of fungi for ectomycorrhizal inoculation in nurseries. Ann. Rev. Phytopathol. 15: 203–222.

    Article  Google Scholar 

  • Trappe, J.M., Molina, M., and Castellano, C. 1984. Reactions of mycorrhizal fungi and mycorrhiza formation to pesticides. Ann. Rev. Phytopathol. 22: 331–359.

    Article  Google Scholar 

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Richter, D.L., Bruhn, J.N. Field survival of containerized red and jack pine seedlings inoculated with mycelial slurries of ectomycorrhizal fungi. New Forest 3, 247–258 (1989). https://doi.org/10.1007/BF00028932

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  • DOI: https://doi.org/10.1007/BF00028932

Key words

  • Cantharellula umbonata
  • Laccaria bicolor
  • outplanting survival
  • reforestation
  • Scleroderma citrinum