Abstract
Tissues of winter-dormant buds were collected from mature Larix decidua, and used for adventitious shoot organogenesis. Tissues of dormant buds merely died on the cytokinin-supplemented, bud-initiation medium. In contrast, buds allowed to flush under greenhouse conditions subsequently yielded approximately two adventitious shoots per bud explant tissue. These shoots elongated normally and several rooted spontaneously. Microbial contamination of in vitro cultures was negligible using explants from buds orgininally collected in a dormant state.
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Diner, A.M. Adventitious micropropagation of mature Larix decidua using dormant versus flushed axillary vegetative buds. New Forest 9, 61–65 (1995). https://doi.org/10.1007/BF00028926
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DOI: https://doi.org/10.1007/BF00028926