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Expression of a phosphoenolpyruvate carboxylase promoter from Mesembryanthemum crystallinum is not salt-inducible in mature transgenic tobacco

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Abstract

The 5′ flanking region of a salt-stress-inducible, CAM-specific phosphoenolpyruvate carboxylase (PEPC) gene from the facultative halophyte Mesembryanthemum crystallinum, was fused to the β-glucuronidase (GUS) reporter gene and introduced into Nicotiana tabacum SR1. The Ppc1 promoter displayed high levels of expression in transgenic tobacco quantitatively and qualitatively similar to a full-length 35S CaMV-GUS construct. Histochemical assays revealed that the full-length Ppc1-GUS fusions expressed GUS activity in all tissues except in root tips. While tobacco is capable of utilizing the Ppc1 cis-acting regulatory regions from M. crystallinum to yield high levels of constitutive expression, this glycophyte fails to direct a stress-inducible pattern of gene expression typical of this promoter in its native, facultative halophytic host.

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References

  1. Bevan M: Binary Agrobacterium vectors for plant transformation. Nucl Acids Res 12: 8711–8721 (1984).

    PubMed  Google Scholar 

  2. Bohnert HJ, Ostrem JA, Cushman JC, Michalowski CB, Rickers J, Meyer G, DeRocher EJ, Vernon DM, Krueger M, Vazquez-Moreno L, Velten J, Hoefner R, Schmitt JM: Mesembryanthemum crystallinum, a higher plant model for the study of environmentally induced changes in gene expression. Plant Mol Biol Rep 6: 10–28 (1988).

    Google Scholar 

  3. Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72: 248–254 (1976).

    Article  PubMed  Google Scholar 

  4. Cushman JC, Meyer G, Michalowski CB, Schmitt JM, Bohnert HJ: Salt stress leads to differential expression of two isogenes of phosphoenolpyruvate carboxylase during Crassulacean acid metabolism induction in the common ice plant. Plant Cell 1: 715–725 (1989).

    Article  PubMed  Google Scholar 

  5. Cushman JC, Bohnert HJ: Salt stress alters A/T-rich DNA-binding factor interactions within the phosphoenolpyruvate carboxylase promoter from Mesembryanthemum crystallinum. Plant Mol Biol, in press (1992).

  6. Dean C, Jones J, Favreau M, Dunsmuir P, Bedbrook J: Influence of flanking sequences on variability in expression levels of an introduced gene in transgenic tobacco plants. Nucl Acids Res 16: 9267–9282 (1988).

    PubMed  Google Scholar 

  7. Horsch RB, Fry JE, Hoffman NL, Eichholtz D, Rogers SG, Fraley RT: A simple and general method for transferring genes into plants. Science 227: 1229–1231 (1985).

    Google Scholar 

  8. Hudspeth RL, Grula JW, Dai Z, Edwards GE, Ku MSB: Expression of maize phosphoenolpyruvate carboxylase in transgenic tobacco. Plant Physiol 98: 458–464 (1992).

    Google Scholar 

  9. Jefferson RA, Kavanagh TA, Bevan MW: GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3907 (1987).

    PubMed  Google Scholar 

  10. Jefferson RA: Assaying chimeric gene in plants: The GUS gene fusion system. Plant Mol Biol Rep 5: 387–405 (1987).

    Google Scholar 

  11. LaRosa PC, Chen Z, Nelson DE, Singh NK, Hasewgawa PM, Bressan RA: Osmotin gene expression is post-transcriptionally regulated. Plant Physiol, in press (1992).

  12. Marcotte WR, Russell SH, Quatrano RS: Abscisic acid responsive sequences from the Em gene of wheat. Plant Cell 1: 969–976 (1989).

    Article  PubMed  Google Scholar 

  13. Matsuoka M, Sanada Y: Expression of photosynthetic genes from the C4 plant, maize, in tobacco. Mol Gen Genet 225: 411–419 (1991).

    Article  PubMed  Google Scholar 

  14. Matzke MA, Primig M, Tnovsky J, Matzke AJM: Reversible methylation and inactivation of marker genes in sequentially transformed tobacco plants. EMBO J 8: 643–649 (1989).

    Google Scholar 

  15. McElwain F, Bohnert HJ, Thomas JC: Light moderates the induction of phosphoenolpyruvate carboxylase by NaCl and abscisic acid in Mesembryanthemum crystallinum. Plant Physiol 99: 1261–1264 (1992).

    Google Scholar 

  16. Nelson DE, Raghothama KG, Singh NK, Hasegawa PM, Bressan RA: Analysis of structure and transcriptional activation of an osmotin gene. Plant Mol Biol 19: 577–588 (1992).

    PubMed  Google Scholar 

  17. Peach C, Velten J: Transgene expression variability (position effect) of CAT and GUS reporter genes driven by linked divergent T-DNA promoters. Plant Mol Biol 17: 49–60 (1991).

    PubMed  Google Scholar 

  18. Vilardell J, Mundy J, Stilling B, Leroux B, Pla M, Freyssinet G, Pages M: Regulation of the maize rabl7 gene promoter in transgenic heterologous systems. Plant Mol Biol 17: 985–993 (1991).

    PubMed  Google Scholar 

  19. Winter K: Crassulacean acid metabolism. In: Barber J, Baker NR (eds) Photosynthetic Mechanisms and the Environment, pp. 329–387. Elsevier, Amsterdam (1985).

    Google Scholar 

  20. Yamaguchi-Shinosaki K, Mino M, Mundy J, Chua N-H: Analysis of an ABA-responsive rice gene promoter in transgenic tobacco. Plant Mol Biol 15: 905–912 (1990).

    PubMed  Google Scholar 

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Author notes

  1. John C. Cushman

    Present address: Department of Biochemistry and Molecular Biology, Oklahoma State University, 74078-0454, Stillwater, OK, USA

Authors and Affiliations

  1. Department of Biochemistry, University of Arizona, 85721, Tucson, AZ, USA

    John C. Cushman & Hans J. Bohnert

  2. Department of Plant Sciences, University of Arizona, 85721, Tucson, AZ, USA

    Michael S. Meiners

  3. Department of Molecular and Cellular Biology, University of Arizona, 85721, Tucson, AZ, USA

    Hans J. Bohnert

Authors
  1. John C. Cushman
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  2. Michael S. Meiners
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  3. Hans J. Bohnert
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Cushman, J.C., Meiners, M.S. & Bohnert, H.J. Expression of a phosphoenolpyruvate carboxylase promoter from Mesembryanthemum crystallinum is not salt-inducible in mature transgenic tobacco. Plant Mol Biol 21, 561–566 (1993). https://doi.org/10.1007/BF00028814

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  • DOI: https://doi.org/10.1007/BF00028814

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