Abstract
An abnormal, structurally modified, kinetically stable S2-state has been reported to be induced when Photosystem II was treated with NaCl-EGTA (or EDTA) in the light or with pH in darkness, both are assumed to release functional Ca2+. In order to compare the mechanism of induction of modified S2-state between the two treatments, effects of illumination during or before low pH-treatment on formation of the abnormal S2-state were investigated by means of thermoluminescence measurements and low temperature EPR spectroscopy. Following results have been obtained: Flash illumination during low pH-treatment did not practically induce the abnormal S2-state, whereas preflash illumination given immediately before low pH-treatment efficiently induced the abnormal S2-state, and its amplitude showed a period-four oscillation on varying the preflash number with maxima at the second and sixth flashes. The abnormal S2-state thus induced by preflashes was identical with the modified S2-state that could be induced in dark-low pH-treated Photosystem II by excitation at 0°C after neutralization to pH 6.5. It was inferred that in low pH-treatment, modified S2-state can be formed from both S2- and S3-states, but its yield from the latter is much higher than from the former, consistent with the early results by Boussac et al. obtained for NaCl-EGTA-light or NaCl-citrate-light treatment.
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Abbreviations
- DCMU:
-
3-(3′,4′-dichlorophenyl)-1,1-dimethylurea
- EDTA:
-
ethylenediaminetetraactate
- EGTA:
-
ethylene glycol bis(β-aminoethylether)-N,N,N′,N′-tetraacetic acid
- Mes:
-
2-(N-morpholino)ethanesulfonic acid
- PS II:
-
Photosystem II
References
Berthold DA, Babcock GT and Yocum CA (1981) A highly resolved, oxygen-evolving Photosystem II preparation from spinach thylakoid membranes. FEBS Lett 134: 231–234
Boussac A and Rutherford AW (1988) Ca2+ binding to the oxygen evolving enzyme varies with the redox state of the Mn cluster. FEBS Lett 236: 432–436
Boussac A, Zimmerman JL and Rutherford AW (1989) EPR signals from modified charge accumulation states of the oxygen evolving enzyme in Ca2+-deficient Photosystem II. Biochemistry 28: 8984–8989
Boussac A, Zimmerman JL and Rutherford AW (1990) Factors influencing the formation of modified S2 EPR signal and the S3 EPR signal in Ca2+-depleted Photosystem II. FEBS Lett 277: 69–74
Butler WL, Visser JWM and Simons HL (1973) The kinetics of light-induced changes of C-550, cytochrome b 559 and fluorescence yield in chloroplasts at low temperature. Biochim Biophys Acta 292: 140–151
Cramer WA and Whitmarsh J (1977) Photosynthetic cytochromes. Ann Rev Plant Physiol 28: 133–172
Debus RJ (1991) The manganese and calcium ions of photosynthetic oxygen evolution. Biochim Biophys Acta, in press
Dismukes GC and Siderer Y (1981) Intermediates of a polynuclear manganese center involved in photosynthetic oxidation of water. Proc Natl Acad Sci USA 78: 247–278
Hansson O and Wydrzynski T (1990) Current perceptions of Photosystem II. Photosynth Res 23: 131–162
Inoue Y (1983) Recent advances in the studies of thermoluminescence of Photosystem II. In: Inoue Y et al. (eds) The Oxygen Evolving System of Photosynthesis, pp 439–450. Academic Press, New York
Kok B, Forbush B and McGloin M (1970) Cooperation of charges in photosynthetic O2 evolution-I. A linear four step mechanism. Photochem Photobiol 11: 457–475
Nugent JHA, Diner BA and Evans MCW (1981) Direct detection of the electron acceptor of Photosystem II. FEBS Lett 124: 241–244
Ono T and Inoue Y (1986) Effects of removal and reconstitution of the extrinsic 33, 24 and 16 kDa proteins on flash oxygen yield in Photosystem II particles. Biochim Biophys Acta 850: 380–389
Ono T and Inoue Y (1988) Discrete extraction of the Ca atom functional for O2 evolution in higher plant Photosystem II by a simple low pH-treatment. FEBS Lett 277: 147–152
Ono T and Inoue Y (1989a) Removal of Ca by pH 3.0 treatment inhibits S2 to S3 transition in photosynthetic oxygen evolution system Biochim Biophys Acta 973: 443–449
Ono T and Inoue Y (1989b) Roles of Ca2+ in O2 evolution in higher plant Photosystem II: Effects of replacement of Ca2+ site by other cations. Arch Biochem Biophys 275: 440–448
Ono T and Inoue Y (1990a) A marked upshift in threshold temperature for the S1-to-S2 transition induced by low pH treatment of PS II membranes. Biochim Biophys Acta 1015: 373–377
Ono T and Inoue Y (1990b) Abnormal redox reactions in photosynthetic O2-evolving centers in NaCl/EDTA-washed PS II. A dark-stable EPR multiline signal and an unknown positive charge accumulator. Biochim Biophys Acta 1020: 269–277
Rutherford AW, Crofts AR and Inoue Y (1982) Thermoluminescence as a probe of Photosystem II photochemistry. The origin of the flash-induced glow peaks. Biochim Biophys Acta 682: 457–465
Sivaraja M, Tso J and Dismukes GC (1989) A calciumspecific site influences the structure and activity of the manganese cluster responsible for photosynthetic water oxidation. Biochemistry 28: 9459–9464
Vass I, Ono T and Inoue Y (1987) Stability and oscillation properties of thermoluminescent charge pairs in the O2-evolving system depleted of CL- or the 33 kDa extrinsic protein. Biochim Biophys Acta 892: 224–235
Vermass WFJ, Renger G and Dohnt G (1984) The reduction of the oxygen-evolving system in chloroplasts by thylakoid components. Biochim Biophys Acta 764: 194–202
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Shen, JR., Ono, Ta. & Inoue, Y. The role of light in formation of modified S2-state upon low pH-treatment of oxygen-evolving Photosystem II. Photosynth Res 32, 71–78 (1992). https://doi.org/10.1007/BF00028799
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DOI: https://doi.org/10.1007/BF00028799