Abstract
A full-length cDNA encoding the precursor of the lumenal 33 kDa oxygen-evolving complex protein from wheat was inserted into a prokaryotic expression vector. Cell-free transcription-translation of this construct generates a precursor protein of the correct size. However, when expressed in Escherichia coli, the protein is quantitatively exported into the perilasm and processed to the mature size. The results indicate that the thylakoid transfer sequence of this precursor can function as an internal E. coli export signal.
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References
Andersson B: Proteins participating in photosynthetic water oxidation. In: Staehelin LA, Arntzen CJ (eds) Photosynthesis III. Encyclopedia of Plant Physiology 19; pp. 447–456. Springer-Verlag, Berlin.
Halpin C, Elderfield PD, James HE, Zimmermann R, Dunbar B, Robinson C: The reaction specificities of the thylakoidal processing peptidase and Escherichia coli leader peptidase are identical. EMBO J 8: 3917–3922 (1989).
James HE, Bartling D, Musgrove JE, Kirwin PM, Herrmann RG, Robinson C: Transport of proteins into chloroplasts. Import and maturation of precursors to 33, 23 and 16 kDa proteins of the oxygen-evolving complex. J Biol Chem 264: 19573–19576 (1989).
Ko K, Cashmore AR: Targeting of proteins to the thylakoid lumen by the pipartite transit peptide of the 33 kd oxygen-evolving protein. EMBO J 8: 3187–3194 (1989).
Meadows JW, Hulford A, Raines CA, Robinson C: Nucleotide sequence of a cDNA clone encoding the precursor of the 33 kDa protein of the oxygen-evolving complex from wheat. Plant Mol Biol 16: 1085–1087 (1991).
Murata N, Miyao M: Extrinsic membrane proteins in the photosynthetic oxygen-evolving complex. Trends Biochem Sci 10: 122–124 (1985).
Pratt JM: Coupled transcription-translation in prokaryotic cell-free systems. In: Hames BD, Higgins SJ (eds) Transcription and Translation: A Practical Approach, pp. 179–209. IRL Press, Oxford (1984).
Seidler A, Michel H: Expression in Escherichia coli of the psbO gene encoding the 33 kD protein of the oxygen-evolving complex from spinach. EMBO J 8: 1743–1748 (1990).
vonHeijne G, Steppuhn J, Herrmann RG: Domain structure of mitochondrial and chloroplast targeting peptides. Eur J Biochem 180: 535–541 (1989).
Westhoff P, Jansson C, Klein-Hitpass K, Berzborn R, Larsson C, Bartlett SG: Intracellular coding sites of polypeptides associated with photosystem II. Plant Mol Biol 4: 137–146 (1985).
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Meadows, J.W., Robinson, C. The full precursor of the 33 kDa oxygen-evolving complex protein of wheat is exported by Escherichia coli and processed to the mature size. Plant Mol Biol 17, 1241–1243 (1991). https://doi.org/10.1007/BF00028739
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DOI: https://doi.org/10.1007/BF00028739