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Oligonucleotide-directed mutagenesis of psbB, the gene encoding CP47, employing a deletion mutant strain of the cyanobacterium Synechocystis sp. PCC 6803

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Abstract

A mutant strain of the cyanobacterium Synechocystis sp. PCC (Pasteur Culture Collection) 6803 has been developed in which psbB, the gene coding for the chlorophyl a-binding protein CP47 in Photosystem II (PSII), has been deleted. This deletion mutant can be used for the reintroduction of modified psbB into the cyanobacterium. To study the role of a large hydrophilic region in CP47, presumably located on the lumenal side of the thylakoid membrane between the fifth and sixth membrane-spanning regions, specific deletions have been introduced in psbB coding for regions within this domain. One psbB mutation leads to deletion of Gly-351 to Thr-365 in CP47, another psbB mutation was targeted towards deletion of Arg-384 to Val-392 in this protein. The deletion from Gly-351 to Thr-365 results in a loss of PSII activity and of photoautotrophic growth of the mutant, but the deletion between Arg-384 and Val-392 retains PSII activity and the ability to grow photoautotrophically. The mutant strain with the deletion from Gly-351 to Thr-365 does not assemble a stable PSII reaction center complex in its thylakoid membranes, and exhibits diminished levels of CP47 and of the reaction center proteins D1 and D2. In contrast to the Arg-384 to Val-392 portion of this domain, the region between Gly-351 and Thr-365 appears essential for the normal structure and function of photosystem II.

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Authors and Affiliations

  1. Department of Botany, Arizona State University, 85287-1601, Tempe, AZ, USA

    Julian J. Eaton-Rye & Wim F. J. Vermaas

  2. Center for the Study of Early Events in Photosynthesis, Arizona State University, 85287-1601, Tempe, AZ, USA

    Julian J. Eaton-Rye & Wim F. J. Vermaas

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  1. Julian J. Eaton-Rye
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  2. Wim F. J. Vermaas
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Eaton-Rye, J.J., Vermaas, W.F.J. Oligonucleotide-directed mutagenesis of psbB, the gene encoding CP47, employing a deletion mutant strain of the cyanobacterium Synechocystis sp. PCC 6803. Plant Mol Biol 17, 1165–1177 (1991). https://doi.org/10.1007/BF00028733

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  • DOI: https://doi.org/10.1007/BF00028733

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