An attempt was made to produce doubled haploids on 16 winter wheat and six spring and winter triticale genotypes thought to carry genes for interspecific incompatibility. The potential for haploid production was maximized by the use of Hordeum bulbosum genotypes selected for high crossability on crossable wheat genotypes, the use of two post-pollination applications of gibberellic acid and by the pollination of immature florets.
A low frequency of seed was set on both the wheat and the triticale genotypes, having mean seed sets of 0.20 per cent and 0.27 per cent respectively. Although the frequency of embryos (‘seed quality’) was high, doubled haploid production was further limited by poor embryo differentiation and regeneration. Haploid plantlets were obtained from the wheat cultivars Moulin and Renard, although successful chromosome doubling and doubled haploid production was achieved in Moulin only.
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Sitch, L.A., Snape, J.W. Doubled haploid production in winter wheat and triticale genotypes, using the Hordeum bulbosum system. Euphytica 35, 1045–1051 (1986). https://doi.org/10.1007/BF00028617
- Hordeum bulbosum
- Triticum aestivum
- doubled haploids
- seed set
- embryo regeneration