Skip to main content
Log in

Isolation of a pure thornless loganberry by meristem tip culture

  • In Vitro Techniques
  • Published:
Euphytica Aims and scope Submit manuscript

Summary

‘Thornless Loganberry’ (TL) is a periclinal chimeral blackberry in which a layer of mutant (thornless) epidermis surrounds a core of wild-type (thorny) tiusse. Due to its chimeral arrangement, TL produces thorny adventitious root cuttings and thorny offspring. To separate the chimera into its components parts, meristems of TL were grow in vitro on modified Murashige and Skoog medium to yield callus and adventitious shoots. One of these shoots has survived, flowered, and produced thornless offspring from seed. The importance of this non-chimeral TL is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Abbreviations

BA:

6-benzylaminopurine

GA3 :

gibberellic acid

NAA:

naphthalene acetic acid

hybridberry:

polyploid bramble interspecific hybrids

MS:

Murashige & Skoog (1962) high mineral salt medium

TL:

‘Thornless Loganberry’

TLtc :

tissue culture-derived (non-chimeral) ‘Thornless Loganberry’

References

  • Anderson, W. C., 1980. Tissue culture propagation of red and black raspberries, Rubus idaeus and R. occidentalis. Acta. Hort. 112: 13–20.

    Google Scholar 

  • Beakbane, A. B., 1935. Blackberries and Loganberries. Proceedings of the Cherries and Soft Fruit Conference Royal Hort. Soc. London, p. 129–132.

  • Beakbane, A. B. & M. V. Labern, 1960. The performance of some virus-tested loganberry clones. Rep. E. Malling Res. Stn. for 1959, p. 48–51.

  • Darrow, G. M., 1937. Blackberry and raspberry improvement. USDA Yearbook of Agriculture, p. 496–533. U.S. Gov't Printing Office, Washington, D.C.

    Google Scholar 

  • Darrow, G. M., 1955. Nature of thornless blackberry sports. Fruit Var. Hort. Digest 10: 14–15.

    Google Scholar 

  • Derman, H., 1947. Histogenesis of some bud sports and variegations. Proc. Amer. Soc. Hort. Sci. 50: 51–73.

    Google Scholar 

  • Camborg, O. L., R. A., Miller & K., Ojima, 1968. Nutrient requirement of suspension cultures of soybean root cells. Expl. Cell Res. 50: 151–158.

    Google Scholar 

  • Hull, J. W., 1958. The development of colchicine-induced and natural polyploid breeding lines in the genus Rubus (Tourn.) L. Ph. D. Thesis, Univ. of Maryland, USA.

  • Hull, J. W. & D. M. Britton, 1958. Development of colchine-induced and natural polyploid breeding lines in the genus rubus L. Maryland Agr. Expt. Stat. Bull. A-91.

  • Jennings, D. L., 1981. A hundred years of Loganberries. Fruit Var. Jour. 35: 34–37.

    Google Scholar 

  • Ke, S., R. M. Skirvin, K. D. McPheeters, A. G. Otterbacher & G. Galletta, 1985. In vitro germination and growth of Rubus embryos. Hort. Science 20: (in press).

  • McPheeters, K. D., 1985. Stability of ‘Thornless Evergreen’ blackberry in vitro and ex vitro. Ph. D. Thesis. University of Illinois, Urbana, USA.

  • McPheeters, K. D. & R. M., Skirvin, 1983. Histogenic layer manipulation in chimeral ‘Thornless Evergreen’ trailing blackberry. Euphytica 32: 351–360.

    Google Scholar 

  • Murashige, T. & F., Skoog, 1962. Revised media for rapid growth and bioassays with tobacco tissue culture. Physiol. Pl. 15: 473–497.

    Google Scholar 

  • Way, D. W., 1966. A comparison of thorned and thornless clones of Logaberry. Rept. E. Malling Res. Stn. p. 116–117.

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hall, H.K., Quazi, M.H. & Skirvin, R.M. Isolation of a pure thornless loganberry by meristem tip culture. Euphytica 35, 1039–1044 (1986). https://doi.org/10.1007/BF00028616

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00028616

Index words

Navigation