Effect of 1-methylcyclopropene and methylenecyclopropane on ethylene binding and ethylene action on cut carnations


1-Methylcyclopropene (1-MCP), formerly designated as Sis-X, has been shown to be an effective inhibitor of ethylene responses in carnation flowers in either the light or the dark. The binding appears to be to the receptor and to be “permanent”. A 6 h treatment at 2.5 nl l−1 is sufficient to protect against ethylene, and 0.5 nl l−1 is sufficient if exposure is for 24 h. As carnation flowers age, a little higher concentration appears to be needed. Most of the natural increase in ethylene production during senescence is prevented by treatment with 1-MCP. A closely related compound, methylenecyclopropane shows ethylene activity. A tritium labelled 1-MCP (60 mCi mmol−1) has been prepared. A higher specific activity is needed for more critical studies.

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  1. 1.

    Borochov, A and Woodson, WR (1989) Physiology and biochemistry of flowers petals senescence. Hort Rev 11: 15–43

    Google Scholar 

  2. 2.

    Brown, JH, Kegge, RL, Sisler, EC, Baker, JE and Thompson, JE (1986) Ethylene binding to senescent carnation petals. J Exp Bot 37: 534–536

    Google Scholar 

  3. 3.

    Burg, SP and Burg, EA (1967) Molecular requirements for the biological activity of ethylene. Plant Physiol 42: 144–152

    Google Scholar 

  4. 4.

    Cotton, FA and Wilkinson, G (1972) Advanced Inorganic Chemistry, A Comprehensive Text. New York: Interscience Publishers

    Google Scholar 

  5. 5.

    Dupille E and Sisler EC (1995) Effect of an ethylene receptor antagonist on carnations and other plant material. In: Ait-Oubahou A and El-Otmani M (eds) Postharvest Physiology, Pathology, and Technologies for Horticultural Commodities: Recent Advances, pp 294–301. Institut Agronomique et Veterinare Hassan II Agadir Morocco

  6. 6.

    Fisher, F and Applequist, DE (1965) Synthesis of 1-methylcyclopropene. J Org Chem 30: 2089–2090

    Google Scholar 

  7. 7.

    Koster, R, Arora, S and Binger, P (1973) Methylenecyclopropan sowie 1-und 3-methylcyclopropen aus methallylchloriden und alkalimetallamiden. Liebigs Ann Chem 1973: 1219–1235

    Google Scholar 

  8. 8.

    Magid, RM, Clarke, TC and Duncan, CD (1971) An efficient and convenient synthesis of 1-methylcyclopropene. J Org Chem 36: 1320–1321

    Google Scholar 

  9. 9.

    Mayak, S, Vaadia, Y and Dilley, DR (1977) Regulation of senescence in carnation (Dianthus caryophyllus) by ethylene. Plant Physiol 59: 591–593

    Google Scholar 

  10. 10.

    Nichols, R (1966) Ethylene production during senescence of flowers. J Hort Sci 41: 279–290

    Google Scholar 

  11. 11.

    Nichols, R (1968) The response of carnations (Dianthus caryophyllus) to ethylene. J Hort Sci 43: 335–349

    Google Scholar 

  12. 12.

    Sisler, EC and Pian, A (1972) Effect of ethylene and cyclic olefins on tobacco leaves. Tob Sci 17: 68–72

    Google Scholar 

  13. 13.

    Sisler, EC (1976) Ethylene analogues; effect of some unsaturated sulphides (thioethers) on tobacco leaves 20: 6–10

    Google Scholar 

  14. 14.

    Sisler, EC (1977) Ethylene activity of some p-acceptor compounds. Tob Sci 21: 43–45

    Google Scholar 

  15. 15.

    Sisler, EC (1979) Measurement of ethylene binding in plant tissue. Plant Physiol 64: 538–542

    Google Scholar 

  16. 16.

    Sisler, EC, Reid, MS and Fujino, DW (1983) Investigation of the mode of action of ethylene in carnation senescence. Acta Hort 141: 229–234

    Google Scholar 

  17. 17.

    Sisler, EC, Reid, MS and Yang, SF (1986) Effect of antagonists of ethylene action on binding of ethylene in cut carnations. Plant Growth Reg 4: 213–218

    Google Scholar 

  18. 18.

    Sisler, EC and Wood, C (1988) Competition of unsaturated compounds and ethylene for binding and action. Plant Growth Reg 7: 181–191

    Google Scholar 

  19. 19.

    Sisler, EC (1990) Ethylene binding receptors—is there more than one? In: Pharis, RP and Roods, SB (eds) Plant Growth Substances, pp 192–200. Berlin: Springer-Verlag Berlin

    Google Scholar 

  20. 20.

    Sisler, EC Blankenship, SM and Guest, M (1990) Competition of cyclooctenes and cyclooctadienes for ethylene binding and activity in plants. Plant Growth Reg 9: 157–164

    Article  Google Scholar 

  21. 21.

    Sisler, EC and Blankenship, SM (1993) Diazocyclopentadiene (DACP), a light sensitive reagent for the ethylene receptor in plants. Plant Growth Reg 12: 125–132

    Google Scholar 

  22. 22.

    Sisler, EC, Blankenship, SM, Fearn, JC and Haynes, R (1993) Effect of diazocyclopentadiene (DACP) on cut carnations. In: Pech, JC, Latche, A, Balague, C (eds) Cellular and Molecular Aspects of the Plant Hormone Ethylene, pp 182–187. Dordrecht: Kluwer Academic Publishers

    Google Scholar 

  23. 23.

    Trewavas, AJ and Jones, AM (1981) Consequences of hormone binding studies for plant growth substance research. What's New in Plant Physiol 12: 5–8

    Google Scholar 

  24. 24.

    Uota, M (1969) Carbon dioxide suppression of ethylene—induced sleepiness of carnation blooms. J Amer Soc Hort Sci 94: 598–601

    Google Scholar 

  25. 25.

    Venis, MA (1985) Hormone Binding Sites in Plants, pp 191. New York: Longman Inc

    Google Scholar 

  26. 26.

    Wang, H and Woodson, WR (1989) Reversible inhibition of ethylene action and interruption of petal senescence in carnation flowers by norbornadiene. Plant Physiol 89: 434–438

    Google Scholar 

  27. 27.

    Woodson, WR (1987) Changes in protein and mRNA populations during the senescence of carnation petals. Physiol Plant 71: 495–502

    Google Scholar 

  28. 28.

    Wu, MJ, Zacarias, L and Reid, MS (1991) Variation in the senescence of carnation (Dianthus caryophyllus L.) cultivars II. Comparison of sensitivity to exogenous ethylene and of ethylene binding. Scientia Hort 48: 109–116

    Article  Google Scholar 

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Sisler, E.C., Dupille, E. & Serek, M. Effect of 1-methylcyclopropene and methylenecyclopropane on ethylene binding and ethylene action on cut carnations. Plant Growth Regul 18, 79–86 (1996). https://doi.org/10.1007/BF00028491

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Key words

  • 1-Methylcyclopropene
  • methylenecyclopropane
  • ethylene
  • carnation
  • Sis-X