Abstract
Plant growth substances (PGSs) were analysed in liquid endosperm of black walnut using HPLC and an ELISA procedure. Of all the PGSs studied, we show no GA3, low levels of cytokinins (io6A, i6Ade, i6Ado) and ABA, and very high level of IAA
Abbreviations
- ABA:
-
Abscisic acid:
- Ade:
-
Adenine:
- GA3 :
-
Gibberellic acid:
- IAA:
-
Indole-3-acetic acid:
- i6Ade:
-
N6(Δ2-1) adenine:
- i6Ado:
-
N6(Δ2-isopentenyl adenosine:
- io6A:
-
Zeatin riboside:
References
Bandurski RS and Schulze A (1977) Concentration of Indole-3-acetic acid and its derivatives in plants. Plant Physiol 60: 211–213
Bewley JD and Black M (1978) Physiology and Biochemistry of Seeds: Development, Germination and Growth, Vol. I, pp 88–101. Berlin, Heidelberg, New York: Springer
Cornu D (1977) La multiplication végétative du noyer hybride: Résultats d'une première campagne, perspectives d'avenir. Rev for fr 29: 457–463
Cornu D (1987) Somatic embryogenesis in tissue cultures of walnut (Juglans nigra, J major and hybrids J. nigra x J. regia). Proceedings of ‘Somatic Cell Genetics of Woody Plants’ IUFRO Working Party S2.04.07, RFA Grosshandsdorf 10–13 August 1987. Martinus Nijhoff Publishers: under press
Eeuwens CJ and Schwabe W (1975) Seed and pod wall development in Pisum sativum in relation to extracted and applied hormones. J exp Bot 26: 1–14
Hofmann M (1987) La culture in vitro de méristèmes de noyers hybrides. D.E.A. de 092 Biologie fondamentale et appliquée, Universités d'Angers, Basançon, Clermont I et II: 30 p
Jay-Allemand C et Cornu D (1986) Culture in vitro d'embryons isolés de noyer commun (Juglans regia L.). Ann Sci For 43: 189–198
Labavitch JM and Polito VS (1985) Fruit growth and development. In: Walnut Orchard Management,. 90–94. Public No. 21410 University of California
Leroux B, Maldiney R, Miginiac E, Sossountzov L and Sotta B (1985) Comparative quantitation of abscisic acid in plant extracts by gas-liquid chromatography and an enzyme-linked immunosorbent assay using the avidin-biotin system. Planta 166: 524–529
Maldiney R, Leroux B, Sabbagh I, Sotta B, Sossountzov L and Miginiac E (1986) A biotin-avidin-based enzyme immunoassay to quantify three phytohormones: Auxin, abscisic acid and zeatin-riboside. Journal of Immunological Methods 90: 151–158
Margara J (1984) Bases de la multiplication végétative: Les méristèmes et l'organogénèse. INRA éditeur: 262 p
McGranahan GH, Driver JA and Tulecke W (1987) Tissue culture of Juglans. In: Cell and Tissue Culture in Forestry, Vol 3, 261–271, JM Bonga and DJ Durzan, ed.
Meynier V (1985) Mise en culture in vitro de méristèmes de noyers hybrides. CR Acad Sc Paris 301 (III) No. 5: 261–263
Sotta B, Pilate G, Pelese F, Sabbagh I, Bonnet M and Maldiney R (1987) An avidin-biotin solid phase ELISA for fentomol isopentenyladenine and isopentenyladenosine measurements in HPLC purified plant extracts. Plant Physiol 84: 571–573
Tulecke W and McGranahan GH (1985) Somatic embryogenesis and plant regeneration from cotyledons of walnut, Juglans regia L. Plant Sci 40: 57–63
VanOverbeek J, Conklin ME and Blakeslece AF (1941) Factors in coconut milk essential for growth and development of very young Datura embryos. Science 94: 350–351
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Label, P., Cornu, D. Determination of plant growth substances in liquid endosperm of immature walnut (Juglans nigra) nuts by an ELISA technique. Plant Growth Regul 7, 209–215 (1988). https://doi.org/10.1007/BF00028243
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00028243