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Identification and cDNA cloning of a new nodule-specific gene, Nms-25 (nodulin-25) of Medicago sativa

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Abstract

A new nodule-specific gene, Nms-25 (nodulin-25), was identified in cDNA clones isolated from a nodule-specific cDNA library of Medicago sativa. The first transcript of this gene appeared 9 days after inoculation of the roots with Rhizobium meliloti. The time of expression and the quantity of the transcripts of the Nms-25 gene was similar to that of leghemoglobin genes suggesting a similar regulation. A protein of 246 amino acids could be deduced from a full-length cDNA clone. The first 24 amino acids at the N-terminal end of this protein formed a signal sequence which might direct membrane transport into the peribacteroid space. Using different predictive methods the signal sequence cleaved protein was tentatively predicted to be a water-soluble enzyme, but not hydrolase.

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Author notes

  1. Éva Vincze

    Present address: Central Food Research Institute, P.O. Box 76, H-1525, Budapest, Hungary

  2. Gábor Tóth

    Present address: Department of Genetics, Eötvös Lóránd University, Múzeum krt. 4/a, H-1088, Budapest, Hungary

Authors and Affiliations

  1. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary

    György Botond Kiss, Éva Vincze, Zoltán Végh & Gábor Tóth

  2. Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary

    József Soós

Authors
  1. György Botond Kiss
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  2. Éva Vincze
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  3. Zoltán Végh
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  4. Gábor Tóth
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  5. József Soós
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Kiss, G.B., Vincze, É., Végh, Z. et al. Identification and cDNA cloning of a new nodule-specific gene, Nms-25 (nodulin-25) of Medicago sativa . Plant Mol Biol 14, 467–475 (1990). https://doi.org/10.1007/BF00027493

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  • DOI: https://doi.org/10.1007/BF00027493

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