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In planta analysis of the Agrobacterium tumefaciens T-cyt gene promoter: identification of an upstream region essential for promoter activity in leaf, stem and root cells of transgenic tobacco

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Abstract

The promoter region of the Agrobacterium tumefaciens T-cyt gene was fused to a β-glucuronidase (gusA) reporter gene and introduced into tobacco plants. Detection of gusA expression in transgenic F1 progeny revealed that the T-cyt promoter is active in many, if not all, cell types in leaves, stems and roots of fully developed plants. Developmental stage-dependent promoter activity was observed in seedlings. Analysis of 5′-deleted promoter fragments showed that sequences located between positions−185 and −139 with respect to the T-cyt translational start codon are essential for T-cyt promoter activity in transfected tobacco protoplasts as well as in transformed tobacco plants.

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Authors and Affiliations

  1. Institute of Molecular Plant Sciences, Leiden University, Clusius Laboratory, Wassenaarseweg 64, 2333 AL, Leiden, Netherlands

    Saskia T. C. Neuteboom, Esther Hulleman, Rob A. Schilperoort & J. Harry C. Hoge

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  1. Saskia T. C. Neuteboom
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  2. Esther Hulleman
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  3. Rob A. Schilperoort
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  4. J. Harry C. Hoge
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Neuteboom, S.T.C., Hulleman, E., Schilperoort, R.A. et al. In planta analysis of the Agrobacterium tumefaciens T-cyt gene promoter: identification of an upstream region essential for promoter activity in leaf, stem and root cells of transgenic tobacco. Plant Mol Biol 22, 923–929 (1993). https://doi.org/10.1007/BF00027379

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  • DOI: https://doi.org/10.1007/BF00027379

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