Abstract
The sequence of an α-tubulin from Prunus amygdalus has been obtained by cDNA cloning. When this sequence is compared to that of the Tubα1 gene from maize it shows a very high degree of similarity, much higher than any of the α-tubulin sequences reported so far from plants. The expression of this gene is high in the stages of seed development where a high divisional activity is present. It is preferentially expressed in the radicular tissues as it is gene Tubα1 in maize. Southern analysis indicates that this gene may from a subfamily of α-tubulin genes having similar sequence and tissue specificity and existing at least in maize and in Prunus.
References
Arumuganathan K, Earle ED: Nuclear DNA content of some important plant species. Plant Mol Biol Rep: 9: 208–218 (1991).
Bernatzky R, Tanksley SD: Towards a saturated linkage map in tomato based on isozymes and random cDNA sequences. Genetics 123: 181–189 (1986).
Carpenter JL, Ploense SE, Snustad DP, Silflow CD: Preferential expression of an α-tubulin gene of Arabidopsis in pollen. Plant Cell 4: 557–571 (1992).
Church GM, Gilbert W: Genomic sequencing. Proc Natl Acad Sci USA 81: 1991–1995 (1984).
Joyce CM, Villemur R, Snustad DP, Silflow CD: Alphatubulin gene expression in maize (Zea mays L.). Changes in isotype expression along the developmental axis of seedling root. J Mol Biol 227: 97–107 (1992).
Kopczak SD, Haas NA, Hussey PJ, Silflow CD, Snustad DP: The small genome of Arabidopsis contains at least six expressed α-tubulin genes. Plant Cell 4: 539–547 (1992).
Liaud MF, Brinkmann H, Cerff R: The β-tubulin gene family of pea: primary structures, genomic organization and intron-dependent evolution of genes. Plant Mol Biol 18: 639–651 (1992).
Logeman J, Schell J, Willmitzer L: Improved method for the isolation of RNA from plant tissues. Anal Biochem 163: 16–20 (1987).
Montoliu L, Puigdomènech P, Rigau J: The tubα3 gene from Zea mays: structure and expression in dividing plant tissues. Gene 94: 201–207 (1990).
Montoliu L, Rigau J, Puigdomènech P: A tandem of alpha-tubulin genes preferentially expressed in radicular tissues from Zea mays. Plant Mol Biol 14: 1–15 (1989).
Montoliu L, Rigau J, Puigdomènech P: Analysis by PCR of the number of homologous genomic sequences to α-tubulin in maize. Plant Sci 84: 179–185 (1992).
Villemur R, Joyce CM, Haas NA, Goddard RH, Kopczak SD, Hussey PJ, Snustad DP, Silflow CD: The α-tubulin gene family of maize (Zea mays L): evidence for two ancient alpha-tubulin genes in plants. J Mol Biol 227: 81–96 (1992).