Abstract
The amino acid sequence of the N-terminal domain of acidic chitinase from unstressed aerial tuber was determined and proved the presence of an N-terminal domain in acidic chitinase. The amino acid sequence was determined on a pyroglutamylaminopeptidase-treated N-terminal fragment of V8 protease and on chymotryptic peptides of this fragment. The sequence determined revealed 8 residues deletion and 2 residues insertion as compared with the N-terminal domain of tobacco basic chitinase. The N-terminal domain determined showed a homology of 40% and 52% with the N-terminal domain of tobacco basic chitinase and wheat germ agglutinin, respectively.
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Abbreviations
- DABITC,4-N,N:
-
dimethylaminoazobenzene 4′-isothiocyanate
- PITC:
-
phenylisothiocyanate
- Cm:
-
carboxymethyl
- WGA:
-
wheat germ agglutinin
- TFA:
-
trifluoroacetic acid
- PGAP:
-
pyroglutamylaminopeptidase
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Araki, T., Funatsu, J., Kuramoto, M. et al. Amino acid sequence of the N-terminal domain of yam (Dioscorea japonica) aerial tuber acidic chitinase. Evidence for the presence of a wheat germ agglutinin domain in matured acidic chitinase from unstressed tuber. Plant Mol Biol 19, 351–354 (1992). https://doi.org/10.1007/BF00027360
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DOI: https://doi.org/10.1007/BF00027360