Skip to main content
SpringerLink
Log in

Chlorophyll a/b-binding protein genes are differentially expressed during soybean development

  • Research Articles
  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

The levels of chlorophyll a/b-binding protein (Cab) gene polysomal poly(A)+ mRNA were quantitated throughout the development of Glycine max L. Cab mRNAs were abundant in young expanding leaves, representing 6.1% of the leaf mRNA population. Lower Cab mRNA levels were present in embryos, stems, and cotyledons of developing seedlings; the lowest levels were found in roots where they accounted for 0.04% of the polysomal poly(A)+ mRNA of this organ. To determine the contribution of different members of the Cab gene family to the Cab mRNA populations, a quantitative S1 nuclease reconstruction assay was developed. Cab3, Cab4, and Cab5 mRNAs were detected in all stages examined during soybean development but their levels underwent differential changes. Cab3 encodes the most abundant Cab mRNA in young leaves, developing embryos, and in Stage VII cotyledons from the developing soybean seedling. The levels of Cab mRNAs were compared to the levels of ribulose-1,5-bisphosphate carboxylase small subunit gene mRNA and differences in their patterns of accumulation were noted. Collectively these data indicate that during soybean embryogenesis developmental control mechanisms supersede light-regulatory signals.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Barkan A, Miles D, Taylor WC: Chloroplast gene expression in nuclear photosynthetic mutants of maize. EMBO J 5: 1421–1427 (1986).

    PubMed  Google Scholar 

  2. Bartholomew DM, Bartley GE, Scolnik PA: Abscisic acid control of rbcS and cab transcription in tomato leaves. Plant Physiol 96: 291–296 (1991).

    Google Scholar 

  3. Bedbrook JR, Smith SM, Ellis RJ: Molecular cloning and sequencing of cDNA encoding the precursor to the small subunit of chloroplast ribulose-1,5-bisphosphate carboxylase. Nature 287: 692–697 (1980).

    Google Scholar 

  4. Chang YC, Walling LL: Spatial and temporal expression of Cab mRNAs in cotyledons of the developing soybean seedling. Planta (accepted) (1991).

  5. Chang YC, Walling LL: Abscisic acid negatively regulates expression of chlorophyll a/b binding protein genes during soybean embryogeny. Plant Physiol 97: 1260–1264 (1991).

    Google Scholar 

  6. Christou O, McCabe DE, Martinell BJ, Swain WF: Soybean genetic engineering — Commercial production of transgenic plants. Trends Biotechnol 8: 145–151 (1990).

    Article  Google Scholar 

  7. Dean C, Pichersky E, Dunsmuir P: Structure evolution, and regulation of RbcS genes in higher plants. Annu Rev Plant Physiol Plant Mol Biol 40: 415–439 (1989).

    Article  Google Scholar 

  8. Dean C, Tamaki S, Dunsmuir P, Favreau M, Katayama C, Dooner H, Bedbrook J: mRNA transcripts of several plant genes are polyadenylated at multiple sites in vivo. Nucl Acids Res 14: 2229–2240 (1986).

    PubMed  Google Scholar 

  9. Demmin DS, Stockinger EJ, Chang YC, Walling LL: Phylogenetic relationships between the chlorophyll a/b binding protein (Cab) multigene family: An intra- and inter-species study. J Mol Evol 29: 266–279 (1989).

    PubMed  Google Scholar 

  10. Feinberg AP, Vogelstein B: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13 (1983).

    PubMed  Google Scholar 

  11. Fluhr R: Regulation of nuclear genes encoding chloroplast proteins in transgenic plants. Devel Genet 11: 197–204 (1990).

    Google Scholar 

  12. Goldberg RB, Barker SJ, Perez-Grau L: Regulation of gene expression during plant embryogenesis. Cell 56: 149–160 (1989).

    Article  PubMed  Google Scholar 

  13. Goldberg RB, Hoschek G, Tam SH, Ditta GS, Breidenbach RW: Abundance, diversity, and regulation of mRNA sequence sets in soybean embryogenesis. Devel Biol 83: 201–217 (1981).

    Google Scholar 

  14. Guiamet JJ Schwartz E, Pichersky E, Nooden LD: Characterization of cytoplasmic and nuclear mutations affecting chlorophyll and chlorophyll-binding proteins during senescence in soybean. Plant Physiol 96: 227–231 (1991).

    Google Scholar 

  15. Jensen KH, Herrin DL, Plumley FG, Schmidt GW: Biogenesis of photosystem II complexes: Transcriptional, translational, and posttranslational regulation. J Cell Biol 103: 1315–1325 (1986).

    Article  PubMed  Google Scholar 

  16. Karlin-Neumann GA, Sun L, and Tobin EM: Expression of light-harvesting chlorophyll a/b-protein genes is phytochrome-regulated in etiolated Arabidopsis thaliana seedlings. Plant Physiol 88: 1323–1331 (1988).

    Google Scholar 

  17. Lamppa G, Morelli G, Chua N-H: Structure and developmental regulation of a wheat gene encoding the major chlorophyll a/b-binding polypeptide. Mol Cell Biol 5: 1370–1378 (1985).

    PubMed  Google Scholar 

  18. Langeridge J, Langeridge P, and Bergquist PL: Extraction of nucleic acid from agarose gels. Anal Biochem 103: 264–271 (1980).

    PubMed  Google Scholar 

  19. Maniatis T, Fritsch EF, Sambrook J: DNA cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1982).

    Google Scholar 

  20. Martineau B, Taylor WC: Photosynthetic gene expression and cellular differentiation in developing maize leaves. Plant Physiol 78: 399–404 (1985).

    Google Scholar 

  21. Medford JI, Sussex IM: Regulation of chlorophyll and Rubisco levels in embryonic cotyledons of Phaseolus vulgaris. Planta 179: 309–315 (1989).

    Google Scholar 

  22. Millar AJ, Kay SA: Circadian control of cab gene transcription and mRNA accumulation in Arabidopsis. Plant Cell 3: 541–550 (1991).

    Article  PubMed  Google Scholar 

  23. Mullet JE: Chloroplast development and gene expression. Annu Rev Plant Physiol 39: 475–502 (1988).

    Article  Google Scholar 

  24. Nelson T, Harpster MH, Mayfield SP, Taylor WC: Light-regulated gene expression during maize leaf development. J Cell Biol 98: 558–564 (1984).

    Article  PubMed  Google Scholar 

  25. Pautot V, Holzer FM, Walling LL: Differential expression of tomato proteinase inhibitor I and II genes during bacterial pathogen invasion and wounding. Mol Plant-Microbe Inter 4: 284–292 (1991).

    Google Scholar 

  26. Piechulla B, Pichersky E, Cashmore AR, Gruissem W: Expression of nuclear and plastid genes for photosynthetic specific proteins during tomato fruit development and ripening. Plant Mol Biol 7: 367–376 (1986).

    Google Scholar 

  27. Saito GY, Chang YC, Walling LL, Thomson WW: Correlation of changes in plastid development ultrastructure and nuclear gene expression during soybean seed ripening. New Phytol 113: 459–469 (1989).

    Google Scholar 

  28. Saito GY, Chang YC, Walling LL, Thomson WW: Chloroplast development and nuclear gene expression in cotyledons of soybean seedlings. New Phytol 114: 547–554 (1990).

    Google Scholar 

  29. Sheen J-Y, Bogorad L: Differential expression of six light-harvesting chlorophyll a/b binding protein genes in maize leaf cell types. Proc Natl Acad Sci USA 83: 7811–7815 (1986).

    PubMed  Google Scholar 

  30. Taylor WC: Regulatory interactions between nuclear and plastid genomes. Annu Rev Plant Physiol Plant Mol Biol 40: 211–233 (1989).

    Article  Google Scholar 

  31. Taylor WC: Transcriptional regulation by a circadian rhythm. Plant Cell 1: 259–264 (1989).

    Article  PubMed  Google Scholar 

  32. Thornber JP: Biochemical characterization and structure of pigment-proteins of photosynthetic organism. In: Staehlelin LA, Arnzten CJ (eds) Encyclopedia of Plant Physiology, New Series, Vol 43, Photosynthesis III: Photosynthetic membranes, pp. 98–114. Springer-Verlag, Berlin (1986).

    Google Scholar 

  33. Walling LL, Chang YC, Demmin DS, Holzer FM: Isolation, characterization, and evolutionary relatedness of three members from the soybean multigene family encoding chlorophyll a/b binding protein genes. Nucl Acids Res 16: 10477–10492 (1988).

    PubMed  Google Scholar 

  34. Walling LL, Drews GN, Goldberg RB: Transcriptional and post-transcriptional regulation of soybean seed protein mRNA levels. Proc Natl Acad Sci USA 83: 2123–2127 (1986).

    Google Scholar 

Download references

Author information

Author notes

  1. Yun C. Chang

    Present address: Department of Genetics, University of Georgia, 30602, Athens, GA, USA

Authors and Affiliations

  1. Department of Botany and Plant Sciences, University of California, 92521, Riverside, CA, USA

    Yun C. Chang & Linda L. Walling

Authors
  1. Yun C. Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Linda L. Walling
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chang, Y.C., Walling, L.L. Chlorophyll a/b-binding protein genes are differentially expressed during soybean development. Plant Mol Biol 19, 217–230 (1992). https://doi.org/10.1007/BF00027343

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00027343

Key words

Search

Navigation