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The identification of leaf thionin as one of the main jasmonate-induced proteins of barley (Hordeum vulgare)

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Abstract

Jasmonic acid (JA) and its methyl ester (JA-Me) are able to introduce the accumulation of several specific polypeptides in cut leaf segments of barley. Two of the most prominent JA-induced proteins of M r 15 000 and 23 000 have been characterized by isolating and sequencing complete cDNA sequences. While the sequence of the M r 23 000 polypeptide shows no similarity to published sequences, the sequence of the M r 15 000 polypeptide corresponds to the higher-molecular-weight precursor of a leaf thionin previously characterized.

Transcripts for the M r 23 000 and M r 15 000 polypeptides accumulate in leaf segments shortly after the beginning of JA treatment. JA and JA-Me induce the appearance of the two proteins not only in leaf segments but also in intact barley seedlings. However, in seedlings the accumulation of JA-induced proteins occurs much more slowly and requires high concentrations of volatile JA-Me. Thus, in barley it seems unlikely that volatile JA-Me is involved in the interaction between different members of this species, as has been proposed recently for tomato seedlings.

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Authors and Affiliations

  1. Botanisches Institut der Universität Kiel, Olshausenstr40-60, 23, Kiel, Germany

    Ingrid Andresen, Walter Becker & Klaus Apel

  2. Institut für Pflanzenwissenschaften der ETH Zürich, Universitätstrasse 2, 8092, Zürich, Switzerland

    Kirsten Schlüter & Klaus Apel

  3. Institut für Biochemie der Pflanzen, Weinberg 3, D-0-4050, Halle/Saale, Germany

    Jan Burges & Benno Parthier

Authors
  1. Ingrid Andresen
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  2. Walter Becker
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  3. Kirsten Schlüter
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  4. Jan Burges
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  5. Benno Parthier
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  6. Klaus Apel
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Andresen, I., Becker, W., Schlüter, K. et al. The identification of leaf thionin as one of the main jasmonate-induced proteins of barley (Hordeum vulgare). Plant Mol Biol 19, 193–204 (1992). https://doi.org/10.1007/BF00027341

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  • DOI: https://doi.org/10.1007/BF00027341

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