Abstract
The gene for the small subunit of ribulose-1,5-bisphosphate carboxylase (Rubisco) is located in the large single-copy region of the plastid genome of the chlorophyll c-containing alga Cryptomonas Φ. The coding sequence is 417 base pairs long, encoding a protein of 139 amino acids, considerably longer than most other small subunit proteins. It is found 83 base pairs downstream from the gene for the large subunit and is cotranscribed with it. An 18 base pair perfect inverted repeat is located 8 base pairs beyond the termination codon. Sequence analysis shows the gene to be more closely related to cyanobacterial and cyanelle small-subunit genes than to those of green algae or land plants. This is the first reported sequence of a Rubisco small-subunit gene which is plastid-encoded and it exhibits a number of unique features. The derived amino acid sequence shows extensive similarity to a partial amino acid sequence from a brown alga, indicating that this gene will be of major interest as a probe for the small subunit genes in other algae and for determining possible evolutionary ancestors of algal plastids.
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Broglie R, Coruzzi G, Lamppa G, Keith B, Chua N-H: Structural analysis of nuclear genes coding for the precursor to the small subunit of wheat ribulose-1,5-biphosphate carboxylase. Bio/technology 1: 55–61 (1983).
Cavalier-Smith TS: The origins of plastids. Biol Linnean Soc 17: 289–306 (1982).
Cavalier-Smith TS: The kingdom Chromista: Origin and Systematics. Progr Phycol Res 4: 309–347 (1986).
Douglas SE: Physical mapping of the plastid genome from the chlorophyll c-containing alga, Cryptomonas Φ. Curr Genet 14: 591–598 (1988).
Feinberg AP, Vogelstein B: A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13 (1983).
Goldschmidt-Clermont M, Rahire M: Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. J Mol Biol 191: 421–432 (1986).
Keen JF, Pappin DJC, Evans LV: Amino acid sequence analysis of the small subunit of ribulose bisphosphate carboxylase from Fucus (Phaeophyceae). J Phycol 207: 324–327 (1988).
Kuhsel M, Kowallik KV: The plastome of a brown alga, Dictyota dichotoma. II. Location of structural genes coding for ribosomal RNAs, the large subunit of ribulose-1,5-biphosphate carboxylase/oxygenase and for polypeptides of photosystems I and II. Mol Gen Genet 207: 361–368 (1987).
Li N, Cattolico RA: Chloroplast genome characterization in th red alga Griffisthia pacifica. Mol Gen Genet 209: 343–351 (1987).
Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).
Marck C: ‘DNA Strider’: a ‘C’ program for the fast analysis of DNA and protein sequences on Apple Macintosh family of computers. Nucleic Acids Res 16: 1829–1835 (1988).
Mazur BJ, Chui CF: Sequence of a genomic clone for the small subunit of ribulose bisphosphate carboxylase/ oxygenase from tobacco. Nucleic Acids Res 13: 2373–2386 (1985).
Meyer TE, Cusanovich MA, Kamen MD: Evidence against use of bacterial amino acid sequence data for construction of all-inclusive phylogenetic trees. Proc Natl Acad Sci USA 83: 217–220 (1986).
Miziorka HM, Lorimer GH: Ribulose-1,5-bisphosphate carboxylase/oxygenase. Ann Rev Biochem 52: 507–535 (1983).
Nierzwicki-Bauer SA, Curtis Se, Haselkorn R: Cotranscription of genes encoding the small and large subunits of ribulose-1,5-bisphosphate carboxylase in the cyanobacterium Anabaena 7120. Proc Natl Acad Sci USA 81: 5961–5965 (1984).
Pribnow D: Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter. Proc Natl Acad Sci USA 72: 784–788 (1975).
Reith M, Cattolico RA: Inverted repeat of Olisthodiscvs luteus chloroplast DNA contains genes for both subunits of ribulose-1,5-bisphosphate carboxylase and the 32000-dalton QB protein: phylogenetic implications. Proc Natl Acad Sci USA 83: 8599–8603 (1986).
Sailland A, Amiri I, Freyssinet G: Amino acid sequence of the ribulose-1,5-bisphosphate carboxylase/oxygenase subunit from Euglena. Plant Mol Biol 7: 213–218 (1986).
Sanger F, Nicklen S, Coulsen AR: Sequencing by chain termination with dideoxynucleotides. Proc Natl Acad Sci USA 74: 5463–5467 (1977).
Shinozaki K, Sugiura M: The gene for the small subunit of ribulose-1,5-bisphosphate is located close to the gene for the large subunit in the cyanobacterium Anacystis nidulans 6301. Nucleic Acids Res 11: 6957–6964 (1983).
Starnes SM, Lambert DH, Maxwell ES, Stevens SE, Porter RD, Shiveley JM: Cotranscription of the large and small subunit genes of ribulose-1,5-bisphosphate carboxylase oxygenase in Cyanophora paradoxa. FEMS Microbiol Lett 28: 165–169 (1985).
Steinmuller K, Kaling M, Zetsche K: In-vitro synthesis of phycobiliproteins and ribulose-1,5-bisphosphate carboxylase by non-polyadenylated-RNA of Cyanidium caldarum and Porphyridium aerugineum. Planta (Berl) 159: 308–313 (1983).
Stern DB, Palmer JD: Extensive and widespread homologies between mitochondrial DNA and chlorol plast DNA in plants. Proc Natl Acad Sci USA 81: 1946–1950 (1984).
Whatley JM, John P, Whatley FR: From extracellular to intracellular: the establishment of mitochondria and chloroplasts. Proc R Soc Lond B204: 165–187 (1979).
Wolter FP, Fritz CC, Willmitzer L, Schell J, Schreier PH: rbcS genes in Solanum tuberosum: conservation of transit peptide and exon shuffling during evolution. Proc Natl Acad Sci USA 85: 846–850 (1988).
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Douglas, S.E., Durnford, D.G. The small subunit of ribulose-1,5-bisphosphate carboxylase is plastid-encoded in the chlorophyll c-containing alga Cryptomonas Φ. Plant Mol Biol 13, 13–20 (1989). https://doi.org/10.1007/BF00027331
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DOI: https://doi.org/10.1007/BF00027331