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Expression of DNA binding proteins in carrot somatic embryos that specifically interact with a cis regulatory element of the French bean phaseolin gene

  • Plant Molecular Biology Update
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Abstract

Nuclear proteins from bean (Phaseolus vulgarus) embryos bind specifically to a 55 bp DNA sequence located upstream of the seed storage protein gene phaseolin. This sequence is capable of elevating gene expression in transgenic tobacco plants by as much as 150-fold when fused to a chimeric β-glucuronidase reporter gene. Results presented in this paper demonstrate that nuclear extracts from carrot embryos bind to a phaseolin DNA sequence that includes a phaseolin activator sequence. This specific DNA binding activity is modulated during somatic embryogenesis. Two separable protein species react specifically with the labeled phaseolin DNA fragment (58.0 and 51.7 dDa). These results suggest that the cis- and trans-acting elements controlling gene expression have been highly conserved during evolution.

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Abbreviations

bp:

base pairs

CAMV:

cauliflower mosaic virus

GUS:

β-glucuronidase

kDa:

kilodalton

SDS-PAGE:

sodium dodecyl sulfate-polyacrylamide gel electrophoresis

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Author notes

  1. Mark J. Guiltinan

    Present address: Department of Biology, University of North Carolina, 27599-3280, Chapel Hill, NC, USA

Authors and Affiliations

  1. Department of Biology, Texas A&M University, 77843-3258, College Station, TX, USA

    Mark J. Guiltinan, Craig L. Nessler & Terry L. Thomas

  2. Biochemistry Department, University of Arizona, 85721, Tucson, AZ, USA

    John C. Thomas

Authors
  1. Mark J. Guiltinan
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  2. John C. Thomas
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  3. Craig L. Nessler
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  4. Terry L. Thomas
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Guiltinan, M.J., Thomas, J.C., Nessler, C.L. et al. Expression of DNA binding proteins in carrot somatic embryos that specifically interact with a cis regulatory element of the French bean phaseolin gene. Plant Mol Biol 13, 605–610 (1989). https://doi.org/10.1007/BF00027322

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  • DOI: https://doi.org/10.1007/BF00027322

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