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Molecular analysis of psb A mutations responsible for various herbicide resistance phenotypes in Synechocystis 6714

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Abstract

Mutations conferring herbicide resistance in 3 mutant strains of the cyanobacterium Synechocystis 6714 have been characterized by gene cloning and sequencing. The mutants display very different phenotypes: DCMU-IIA is DCMU-resistant and atrazine-resistant, DCMU-IIB is DCMU-resistant and atrazine-sensitive, and Az-V is DCMU-sensitive, atrazine-resistant and presents particular photoinhibition properties. These mutants were originally obtained either by one-step selection (DCMU-IIA) or by two-step selection (DCMU-IIB and Az-V). psbA copies carrying herbicide resistance have been identified by transformation experiments as psbAI in all cases. Sequences of the psbAI copy of each mutant have been compared to the wild-type sequence. In the single mutant DCMU-IIA, a point mutation at codon 264 (Ser→Ala) results in resistance to both DCMU and atrazine. In the double mutants DCMU-IIB and Az-V, two point mutations were found. DCMU-IIB was derived from DCMU-IIA and had acquired a second mutation at codon 255 (Phe→Leu) resulting in a slight increase in DCMU resistance and complete abolition of atrazine resistance. Az-V contains two changes at codons 211 (Phe→Ser) and 251 (Ala→Val) resulting in high atrazine resistance but only slight DCMU resistance.

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Abbreviations

DCMU::

3-(3,4-dichlorophenyl)-1,1-dimethylurea

PSII::

photosystem II

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Authors and Affiliations

  1. UPR 407, CNRS, 91198, Gif sur Yvette, France

    G. Ajlani, D. Kirilovsky, M. Picaud & C. Astier

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  1. G. Ajlani
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  2. D. Kirilovsky
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  3. M. Picaud
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  4. C. Astier
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Ajlani, G., Kirilovsky, D., Picaud, M. et al. Molecular analysis of psb A mutations responsible for various herbicide resistance phenotypes in Synechocystis 6714. Plant Mol Biol 13, 469–479 (1989). https://doi.org/10.1007/BF00027307

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  • DOI: https://doi.org/10.1007/BF00027307

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