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The role of the vacuole in the accumulation and mobilization of sucrose

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Abstract

The contribution that isolated vacuoles have made to understanding sucrose storage and mobilization is reviewed briefly, with particular reference to the storage root of red beet (Beta vulgaris L.). Work with isolated vacuoles has shown that in this tissue sucrose is confined to the vacuole and some progress has been made in elucidating the possible mechanism of sucrose transport into the vacuole. The evidence that this is a H+: sucrose antiport, dependent on the activity of a proton-translocating ATPase is examined. It is concluded that while there is some evidence for the presence of a proton pump, a link between this and sucrose uptake has still to be established. Using isolated vacuoles it has been demonstrated that during mobilization of sucrose, hydrolysis occurs within the vacuole but the mechanism of unloading of hexoses from the vacuole remains to be elucidated.

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References

  1. Admon A, Jacoby B and Goldschmidt EE (1980) Assessment of cytoplasmic contamination in isolated vacuole preparations. Plant Physiol 65:85–87

    Google Scholar 

  2. Admon A, Jacoby B and Goldschmidt EE (1981) Some characteristics of the Mg-ATPase of isolated red beet vacuoles. Plant Sci Lett 22:89–96

    Google Scholar 

  3. Bacon JSD, MacDonald IR and Knight AH (1965) The development of invertase activity in slices of the root of Beta vulgaris L. washed under asceptic conditions. Biochem J 94:175–182

    Google Scholar 

  4. Bennett AB and Spanswick RM (1983) Optical measurements of ΔpH and Δψ in corn root membrane vesicles: Kinetic analysis of Cl− effects on proton-translocating ATPase. J Membrane Biol 71:95–107

    Google Scholar 

  5. Boller T and Kende H (1979) Hydrolytic enzymes in the central vacuole of plant cells. Plant Physiol 63:1123–1132

    Google Scholar 

  6. Briskin DP and Leonard RT (1980) Isolation of tonoplast vesicles from tobacco protoplasts. Plant Physiol 66:684–687

    Google Scholar 

  7. Brown SC and Coombe BG (1982) Sugar transport by an enzyme complex at the tonoplast of grape pericarp cells? Naturwissenschaften 69:42–43

    Google Scholar 

  8. Butcher HC, Wagner GJ and Siegelman HW (1977) Location of acid hydrolases in protoplasts: Examination of the proposed lysosomal function of the mature vacuole. Plant Physiol 59:1098–1103

    Google Scholar 

  9. Cretin H (1982) The proton gradient across the vacuo-lysosomal membrane of lutoids from Hevea brasiliensis. I. Further evidence for a proton-translocating ATPase on the vacuo-lysosomal membrane of intact lutoids. J Membrane Biol 65:175–184

    Google Scholar 

  10. d'Auzac J (1977) ATPase membranaire de vacuoles lysosomales: Les lutoides du latex d'Hevea brasiliensis. Phytochem 16:1881–1885

    Google Scholar 

  11. Doll S and Hauer R (1981) Determination of the membrane potential of vacuoles isolated from red-beet storage tissue: Evidence for an electrogenic ATPase. Planta 152:153–158

    Google Scholar 

  12. Doll S, Rodier F and Willenbrink J (1979) Accumulation of sucrose in vacuoles isolated from red beet tissue. Planta 144:407–411

    Google Scholar 

  13. Giaquinta R (1977) Sucrose hydrolysis in relation to phloem translocation in Beta vulgaris. Plant Physiol 60:339–343

    Google Scholar 

  14. Guy M, Reinhold L and Michaeli D (1979) Direct evidence for a sugar transport mechanism in isolated vacuoles. Plant Physiol 64:61–64

    Google Scholar 

  15. Komor E, Thom M and Maretzki A (1982) Vacuoles from sugarcane suspension cultures. III. Protonmotive potential difference. Plant Physiol 69:1326–1330

    Google Scholar 

  16. Leigh RA (1983) Methods, progress and potential for the use of isolated vacuoles in studies of solute transport in higher plant cells. Physiol Plant 57:390–396

    Google Scholar 

  17. Leigh RA, apRees T, Fuller WA and Banfield J (1979) The location of acid invertase activity and sucrose in vacuoles isolated from storage roots of red beet (Beta vulgaris L.). Biochem J 178:539–547

    Google Scholar 

  18. Leigh RA and Branton D (1976) Isolation of vacuoles from root storage tissue of Beta vulgaris L. Plant Physiol 58:656–662

    Google Scholar 

  19. Leigh RA, Branton D and Marty F (1979) Methods for the isolation of intact vacuoles and fragments of tonoplast. In: Reid E, ed. Plant Organelles, pp 69–80. Chichester: Ellis Horwood Ltd

    Google Scholar 

  20. Leigh RA and Tomos AD (1983) An attempt to use isolated vacuoles to determine the distribution of sodium and potassium in cells of storage roots of red beet (Beta vulgaris L.). Planta 159:469–475

    Google Scholar 

  21. Leigh RA and Walker RR (1980) ATPase and acid phosphatase activities associated with vacuoles isolated from storage roots of red beet (Beta vulgaris L.). Planta 150:222–229

    Google Scholar 

  22. Lin W, Wagner GJ, Siegelman HW and Hind G (1977) Membrane-bound ATPase of intact vacuoles and tonoplasts isolated from mature plant tissue. Biochim Biophys Acta 465:110–117

    Google Scholar 

  23. Marin B, Marin-Lanza M and Komor E (1981) The protonmotive potential difference across the vacuo-lysosomal membrane of Hevea brasiliensis (rubber tree) and its modification by a membrane-bound adenosine triphosphatase. Biochem J 198: 365–372

    Google Scholar 

  24. Miller AJ, Brimelow JJ and John P (1984) Membrane potential changes in vacuoles from storage roots of red beet (Beta vulgaris L.). Planta 160:59–65

    Google Scholar 

  25. Nishimura M and Beevers H (1978) Hydrolases in vacuoles from castor bean endosperm. Plant Physiol 62:44–48

    Google Scholar 

  26. O'Neill S, Bennett AB and Spanswick RM (1983) Characterization of a NO3 −-sensitive H+-ATPase from corn roots. Plant Physiol 72:837–846

    Google Scholar 

  27. Roberts JKM, Ray PM, Wade-Jardetzky N and Jardetzky O (1980) Estimation of cytoplasmic and vacuolar pH in higher plant cells by 31P NMR. Nature 283: 870–872

    Google Scholar 

  28. Rona J-P, Cornel D, Grignon C and Heller R (1982) The electrical potential difference across the tonoplast of Acer pseudoplatanus cells. Physiol Vég 20:459–463

    Google Scholar 

  29. Rona J-P, Pitman MG, Lüttge U and Ball E (1980) Electrochemical data on compartmentation in cell wall, cytoplasm, and vacuole in leaf cells in the CAM genus Kalanchoë. J Membrane Biol 57:25–35

    Google Scholar 

  30. Thom M, Momor E and Maretzki A (1982) Vacuoles from sugarcane suspension cells. II. Characterization of sugar uptake. Plant Physiol 69:1320–1325

    Google Scholar 

  31. Thom M, Maretzki A and Komor E (1982) Vacuoles from sugarcane suspension cells. I. Isolation and partial characterization. Plant Physiol 69:1315–1319

    Google Scholar 

  32. Thom M, Willenbrink J and Maretzki A (1983) Characteristics of ATPase from sugarcane protoplast and vacuole membranes. Physiol Plant 58:497–504

    Google Scholar 

  33. Wagner GJ (1979) Content and vacuole/extravacuole distribution of neutral sugars, free amino acids and anthocyanins in protoplasts. Plant Physiol 64: 88–93

    Google Scholar 

  34. Wagner GJ (1983) Higher plant vacuoles and tonoplasts. In: Hall JL and Moore AL, eds. Isolation of Membranes and Organelles from Plant Cells, pp 55–81. London: Academic Press

    Google Scholar 

  35. Wagner GJ and Lin W (1982) An active proton pump of intact vacuoles isolated from Tulipa petals. Biochim Biophys Acta 689:261–266

    Google Scholar 

  36. Wagner GJ and Mulready P (1983) Characterization and solubilization of nucleotide-specific Mg2+-ATPase and Mg2+-pyrophosphatase of tonoplast. Biochim Biophys Acta 728:267–280

    Google Scholar 

  37. Wagner GJ and Siegelman HW (1975) Large-scale isolation of intact vacuoles and isolation of chloroplasts from protoplasts of mature plant tissues. Science 190: 1298–1299

    Google Scholar 

  38. Wagner W, Keller F and Wiemken A (1983) Fructan metabolism in cereals: Induction in leaves and compartmentation in protoplasts and vacuoles. Z Pflanzenphysiol 112:359–372

    Google Scholar 

  39. Walker RR and Leigh RA (1981) Characterization of a salt-stimulated ATPase activity associated with vacuoles from storage roots of red beet (Beta vulgaris L.). Planta 153:140–149

    Google Scholar 

  40. Willenbrink J and Doll S (1979) Characteristics of sucrose uptake system of vacuoles isolated from red beet tissue: Kinetics and specificity of the sucrose uptake system. Planta 147:159–162

    Google Scholar 

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  1. Rothamsted Experimental Station, AL5 2JQ, Harpenden, Hertfordshire, UK

    Roger A. Leigh

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  1. Roger A. Leigh
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Leigh, R.A. The role of the vacuole in the accumulation and mobilization of sucrose. Plant Growth Regul 2, 339–346 (1984). https://doi.org/10.1007/BF00027293

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  • DOI: https://doi.org/10.1007/BF00027293

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