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Tracheary element differentiation in Zinnia mesophyll cell cultures

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Abstract

Mechanically isolated mesophyll cells of Zinnia elegans differentiate into tracheary elements (TEs) when cultured in a medium containing adequate auxin and cytokinin. Differentiation in this culture system is relatively synchronous, rapid (occuring within 3 days of cell isolation) and efficient (with up to 65% of the mesophyll cells differentiating into TEs), and does not require prior mitosis. The Zinnia system has been used to investigate (a) cytological and ultrastructural changes occurring during TE differentiation, such as the reorganization of microtubules controlling secondary wall deposition, (b) the influences of calcium and of various plant hormones and antihormones on TE differentiation, and (c) biochemical changes during differentiation, including those occurring during secondary wall deposition, lignification and autolysis. This review summarizes experiments in which the Zinnia system has served as a model for the study of TE differentiation.

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References

  1. Aloni R (1988) Vascular differentiation within the plant. In: LW Roberts, PB Gahan and R Aloni. Vascular Differentiation and Plant Growth Regulators, 39–62. Berlin, Heidelberg: Springer-Verlag

    Google Scholar 

  2. Bengochea T, Harry GI and Dodds JH (1983) Cytodifferentiation of xylem cells in vitro: an overview. Histochem J 15: 411–418

    PubMed  Google Scholar 

  3. Beresford WA (1990) Direct transdifferentiation: can cells change their phenotype without dividing? Cell Diff Dev 29: 81–93

    Article  Google Scholar 

  4. Burgess J and Linstead P (1984) In-vitro tracheary element formation: structural studies and the effect of tri-iodobenzoic acid. Planta 160: 481–489

    Google Scholar 

  5. Burgess J and Linstead P (1984) Comparison of tracheary element differentiation in intact leaves and isolated mesophyll cells of Zinnia elegans. Micron Microscopica Acta 15: 153–160

    Article  Google Scholar 

  6. Church DL and Galston AW (1988) Hormonal induction and antihormonal inhibition of tracheary element differentiation in Zinnia cell cultures. Phytochem 27: 2435–2439

    Article  Google Scholar 

  7. Church DL and Galston AW (1988) Kinetics of determination in the differentiation of isolated mesophyll cells of Zinnia elegans to tracheary elements. Plant Physiol 88: 92–96

    PubMed  Google Scholar 

  8. Church DL and Galston AW (1988) 4-Coumarate: coenzyme A ligase and isoperoxidase expression in Zinnia mesophyll cells induced to differentiate into tracheary elements. Plant Physiol 88: 679–684

    PubMed  Google Scholar 

  9. Church DL and Galston AW (1989) Correction. Plant Physiol 90: 365

    Google Scholar 

  10. Church DL and Galston AW (1989) Hormonal induction of vascular differentiation in cultured Zinnia leaf disks. Plant Cell Physiol 30: 73–78

    Google Scholar 

  11. Dalessandro G and Roberts LW (1971) Induction of xylogenesis in pith parenchyma explants of Lactuca. Am J Bot 58: 378–385

    Google Scholar 

  12. Delmer DP (1987) Cellulose biosynthesis. Ann Rev Plant Physiol 38: 259–290

    Google Scholar 

  13. Dodds JH (1980) The effect of 5-fluorodeoxyuridine and colchicine on tracheary element differentiation in isolated mesophyll cells of Zinnia elegans L. Z Pflanzenphysiol 99: 283–285

    Google Scholar 

  14. Dodds JH (1981) Relationship of the cell cycle to xylem cell differentiation: A new model. Plant Cell Env 4: 145–146

    Google Scholar 

  15. Esau K (1965) Vascular Differentiation in Plants. New York: Holt, Rinehart and Winston

    Google Scholar 

  16. Falconer MM, Donaldson G and Seagull RW (1988) MTOCs in higher plant cells: an immunofluorescent study of microtubule assembly sites following depolymerization by APM. Protoplasma 144: 46–55

    Google Scholar 

  17. Falconer MM and Seagull RW (1985) Immunofluorescent and Calcofluor White staining of developing tracheary elements in Zinnia elegans L. suspension cultures. Protoplasma 125: 190–198

    Google Scholar 

  18. Falconer MM and Seagull RW (1985) Xylogenesis in tissue culture: Taxo effects on microtubule reorientation and lateral association in differentiating cells. Protoplasma 128: 157–166

    Google Scholar 

  19. Falconer MM and Seagull RW (1986) Xylogenesis in tissue culture II: microtubules, cell shape and secondary wall patterns. Protoplasma 133: 140–148

    Google Scholar 

  20. Falconer MM and Seagull RW (1987) Amiprophosmethyl (APM): a rapid, reversible, anti-microtubule agent for plant cell cultures. Protoplasma 136: 118–124

    Google Scholar 

  21. Falconer MM and Seagull RW (1988) Xylogenesis in tissue culture III: continuing wall deposition during tracheary element development. Protoplasma 144: 10–16

    Google Scholar 

  22. Fowke LC (1987) Investigations of cell structure using cultured cells and protoplasts. In: CE Green, DA Somers, WP Hackett and DD Biesboer, eds. Plant Tissue and Cell Culture, 17–31. New York: Alan R. Liss

    Google Scholar 

  23. Fukuda H (1987) A change in tubulin synthesis in the process of tracheary element differentiation and cell division of isolated Zinnia mesophyll cells. Plant Cell Physiol 28: 517–528

    Google Scholar 

  24. Fukuda H (1989) Regulation of tubulin degradation in isolated Zinnia mesophyll cells in culture. Plant Cell Physiol 30: 243–252

    Google Scholar 

  25. Fukuda H (1989) Cytodifferentiation in isolated single cells. Bot Mag (Tokyo) 102: 491–501

    Google Scholar 

  26. Fukuda H and Kobayashi H (1989) Dynamic organization of the cytoskeleton during tracheary-element differentiation. (Review) Dev Growth Diff 31: 9–16

    Google Scholar 

  27. Fukuda H and Komamine A (1980) Establishment of an experimental system for the study of tracheary element differentiation from single cells isolated from the mesophyll of Zinnia elegans. Plant Physiol 65: 57–60

    Google Scholar 

  28. Fukuda H and Komamine A (1980) Direct evidence for cytodifferentiation to tracheary elements without intervening mitosis in a culture of single cells isolated from the mesophyll of Zinnia elegans. Plant Physiol 65: 61–64

    Google Scholar 

  29. Fukuda H and Komamine A (1981) Relationship between tracheary element differentiation and the cell cycle in single cells isolated from the mesophyll of Zinnia elegans. Physiol Plant 52: 423–430

    Google Scholar 

  30. Fukuda H and Komamine A (1981) Relationship between tracheary element differentiation and DNA synthesis in single cells isolated from the mesophyll of Zinnia elegans—analysis by inhibitors of DNA synthesis. Plant Cell Physiol 22: 41–49

    Google Scholar 

  31. Fukuda H and Komamine A (1982) Lignin synthesis and its related enzymes as markers of tracheary-element differentiation in single cells isolated from the mesophyll of Zinnia elegans. Planta 155: 423–430

    Google Scholar 

  32. Fukuda H and Komamine A (1983) Changes in the synthesis of RNA and protein during tracheary element differentiation in single cells isolated from the mesophyll of Zinnia elegans. Plant Cell Physiol 24: 603–614

    Google Scholar 

  33. Fukuda H and Komamine A (1985) Cytodiffereniation. In: IK Vasil, ed. Cell Culture and Somatic Cell Genetics of Plants, Vol 2, 149–212. Orlando: Academic Press

    Google Scholar 

  34. Gahan PB (1988) Xylem and phloem differentiation in perspective. In: LW Roberts, PB Gahan and R Aloni. Vascular Differentiation and Plant Growth Regulators, 1–21. Berlin, Heidelberg: Springer-Verlag

    Google Scholar 

  35. Grisebach H (1981) Lignins. In: EE Conn, ed. The Biochemistry of Plants, Vol 7, 457–478. London: Academic Press

    Google Scholar 

  36. Haigler CH and Brown RM (1986) Transport of rosettes from the Golgi apparatus to the plasma membrane in isolated mesophyll cells of Zinnia elegans during differentiation to tracheary elements in suspension culture. Protoplasma 134: 111–120

    Google Scholar 

  37. Hepler PK and Wayne RO (1985) Calcium and plant development. Ann Rev Plant Physiol 36: 397–439

    Google Scholar 

  38. Ingold E, Sugiyama M and Komamine A (1988) Secondary cell wall formation: changes in cell wall constitutents during the differentiation of isolated mesophyll cells of Zinnia elegans to tracheary elements. Plant Cell Physiol 29: 295–303

    Google Scholar 

  39. Ingold E, Sugiyama M and Komamine A (1990) L-α-aminooxy-β-phenylpropionic acid inhibits lignification but not the differentiation to tracheary elements of isolated mesophyll cells of Zinnia elegans. Physiol Plant 78: 67–74

    Article  Google Scholar 

  40. Iwasaki T, Fukuda H and Shibaoka H (1986) Inhibition of cell division and DNA synthesis by gibberellin in isolated Zinnia mesophyll cells. Plant Cell Physiol 27: 717–724

    Google Scholar 

  41. Iwasaki T, Fukuda H and Shibaoka H (1988) Relationship between DNA synthesis and the increase in the level of tubulin during dedifferentiation of isolated Zinnia mesophyll cells. Protoplasma 143: 130–138

    Google Scholar 

  42. Jones DH (1984) Phenylalanine ammonia-lyase: Regulation of its induction, and its role in plant development. Phytochem 23: 1349–1359

    Article  Google Scholar 

  43. Kobayashi H, Fukuda H and Shibaoka H (1987) Reorganization of actin filaments associated with the differentiation of tracheary elements in Zinnia mesophyll cells. Protoplasma 138: 69–71

    Google Scholar 

  44. Kobayashi H, Fukuda H and Shibaoka H (1988) Interrelation between the spatial disposition of actin filaments and microtubules during the differentiation of tracheary elements in cultured Zinnia cells. Protoplasma 143: 29–37

    Google Scholar 

  45. Kohlenbach HW (1984) Culture of isolated mesophyll cells. In: IK Vasil, ed. Cell Culture and Somatic Cell Genetics of Plants, Vol 1, 204–212. Orlando: Academic Press

    Google Scholar 

  46. Kohlenbach HW (1985) Cytodifferentiation. In: LC Fowke and F Constabel, eds. Plant Protoplasts, 91–104. Boca Raton: CRC Press

    Google Scholar 

  47. Kohlenbach HW, Körber M and Li L-C (1982) Cytodifferentiation of protoplasts isolated from a stem embryo system of Brassica napus to tracheary elements. Z Pflanzenphysiol 107: 367–371

    Google Scholar 

  48. Kohlenbach HW and Schmidt B (1975) Cytodifferenzierung in Form einer direkten Umwandlung isolierter Mesophyllzellen zu Tracheiden (Cytodifferentiation in the mode of a direct transformation of isolated mesophyll cells to tracheids). Z Pflanzenphysiol 75: 369–374

    Google Scholar 

  49. Kohlenbach HW and Schöpke C (1981) Cytodifferentiation to tracheary elements from isolated mesophyll protoplasts of Zinnia elegans. Naturwissenschaften 68: 576–577

    Google Scholar 

  50. Lin Q and Northcote DH (1990) Expression of phenylalanine ammonia-lyase gene during trachearyelement differentiation from cultured mesophyll cells of Zinnia elegans L. Planta 182: 591–598

    Google Scholar 

  51. Lloyd CW and Seagull RW (1985) A new spring for plant cell biology: microtubules as dynamic helices. Trends Biochem 10: 476–478

    Article  Google Scholar 

  52. Masuda H, Fukuda H and Komamine A (1983) Changes in peroxidase isoenzyme patterns during tracheary element differentiation in a culture of single cells isolated from the mesophyll of Zinnia elegans. Z. Pflanzenphysiol 112: 417–426

    Google Scholar 

  53. Meinhardt H (1984) Models of pattern formation and their application to plant development. In: PW Barlow and DJ Carr, eds. Positional Controls in Plant Development, 1–32 Cambridge: Cambridge University Press

    Google Scholar 

  54. Northcote DH (1985) Cell organelles and their function in biosynthesis of cell wall components: Control of cell wall assembly during differentiation. In: T Higuchi, ed. Biosynthesis and Biodegradation of Wood Components, 87–108. New York: Academic Press

    Google Scholar 

  55. Phillips R (1980) Cytodifferentiation. In: IK Vasil, ed. Perspectives in Plant and Cell Tissue Culture, Int Rev Cytol (Suppl) Vol 11A, 55–70. New York: Academic Press

    Google Scholar 

  56. Roberts AW and Haigler CH (1989) Rise in chlorotetracycline fluorescence accompanies tracheary element differentiation in suspension cultures of Zinnia. Protoplasma 152: 37–45

    Google Scholar 

  57. Roberts AW and Haigler CH (1990) Tracheary-element differentiation in suspension-cultured cells of Zinnia requires uptake of extracellular Ca2+. Experiments with calcium-channel blockers and calmodulin inhibitors. Planta 180: 502–509

    Google Scholar 

  58. Roberts AW, Koonce LT and Haigler CH (1992) A simplified medium for in vitro tracheary element differentiation in mesophyll suspension cultures from Zinnia elegans L. Plant Cell, Tissue, Organ Culture 28: 27–35

    Google Scholar 

  59. Roberts LW (1976) Cytodifferentiation in Plants: Xylogenesis as a Model System. Cambridge: Cambridge University Press

    Google Scholar 

  60. Roberts LW (1988) Hormonal Aspects of Vascular Differentiation. In: LW Roberts, PB Gahan and R Aloni. Vascular Differentiation and Plant Growth Regulators, 22–38. Berlin, Heidelberg: Springer-Verlag

    Google Scholar 

  61. Roberts LW (1988) Evidence from Wound Responses and Tissue Cultures. In: LW Roberts, PB Gahan and R Aloni. Vascular Differentiation and Plant Growth Regulators, 63–88. Berlin, Heidelberg: Springer-Verlag

    Google Scholar 

  62. Sachs T (1981) The control of the patterned differentiation of vascular tissues. Adv Bot Res 9: 152–262

    Google Scholar 

  63. Seeni S, Mariappan T, Gopalan G and Gnanam A (1983) Mechanical separation of palisade and spongyparenchyma cells from the leaves of mesomorphic dicotyledons for photosynthetic studies. Planta 157: 105–110

    Google Scholar 

  64. Siegel SM (1956) The biosynthesis of lignin; evidence for the participation of cellulose as sites for oxidative polymerization of eugenol. J Am Chem Soc 78: 1753–1755

    Google Scholar 

  65. Sugiyama M, Fukuda H and Komamine A (1986) Effects of nutrient limitation and γ-irradiation on tracheary element differentiation and cell division in single mesophyll cells of Zinnia elegans. Plant Cell Physiol 27: 601–606

    Google Scholar 

  66. Sugiyama M, Fukuda H and Komamine A (1990) Characteristics of the inhibitory effect of 5-fluorodeoxyuridine on cytodifferentiation into tracheary elements of isolated mesophyll cells of Zinnia elegans. Plant Cell Physiol 31: 61–67

    Google Scholar 

  67. Sugiyama M and Komamine A (1987) Effects of inhibitors of ADP-ribosyltransferase on the differentiation of tracheary elements from isolated mesophyll cells of Zinnia elegans. Plant Cell Physiol 28: 541–544

    Google Scholar 

  68. Sugiyama M and Komamine A (1987) Relationship between DNA synthesis and cytodifferentiation to tracheary elements. Oxford Surveys of Plant Mol Cell Biol 4: 343–346

    Google Scholar 

  69. Sugiyama M and Komamine A (1990) Transdifferentiation of quiescent parenchymatous cells into tracheary elements. (Review) Cell Diff Dev 31: 77–87

    Article  Google Scholar 

  70. Suzuki K, Ingold E, Sugiyama M and Komamine A (1991) Xylan synthase activity in isolated mesophyll cells of Zinnia elegans during differentiation to tracheary elements. Plant Cell Physiol 32: 303–306

    Google Scholar 

  71. Thelen MP and Northcote DH (1989) Identification and purification of a nuclease from Zinnia elegans L.: a potential marker for xylogenesis. Planta 179: 181–195

    Google Scholar 

  72. Torrey JG (1975) Tracheary element formation from single isolated cells in culture. Physiol Plant 35: 158–165

    Google Scholar 

  73. Torrey JG, Fosket DE and Hepler PK (1971) Xylem formation: A paradigm of cytodifferentiation in higher plants. Am Sci 59: 338–352

    Google Scholar 

  74. Turgeon R (1975) Differentiation of wound vessel members without DNA synthesis, mitosis or cell division. Nature (London) 257: 806–808

    Google Scholar 

  75. Woolhouse HW (1984) Senescence in plant cells. In: I Davies and DC Sigee, eds. Cell Ageing and Cell Death, Society for Experimental Biology Seminar Series, Vol 25, 123–154. Cambridge: Cambridge University Press

    Google Scholar 

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  1. Diane L. Church

    Present address: Dept. of Biological Sciences, Dartmouth College, 03755, Hanover, NH, USA

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  1. Department of Botany, University of Wisconsin, 430 Lincoln Drive, 53706, Madison, WI, USA

    Diane L. Church

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Church, D.L. Tracheary element differentiation in Zinnia mesophyll cell cultures. Plant Growth Regul 12, 179–188 (1993). https://doi.org/10.1007/BF00027197

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