Abstract
WIS-2-1A, a 8624 bp insertion in the Glu-1A-2 locus of chromosome 1A of wheat, consists of two 1755 bp long terminal repeats enclosing a 5114 bp internal region. No long open reading frames could be found, but inspection of the predicted amino acid sequence showed regions with homology to retrotransposon structures, including a methionine tRNA initiator binding site, a nucleotide binding domain, a protease, an integrase and a polymerase. DNA replication errors have resulted in frame-shifts in the protein coding region, suggesting that retrotransposition of WIS-2-1A, if it occurs, must be mediated by trans-acting factors.
This is a preview of subscription content, log in via an institution to check access.
References
Camirand A, Brisson N: The complete nucleotide sequence of the Ts1 retrotransposon of potato. Nucl Acids Res 18: 4929 (1990).
Clare J, Farabaugh P: Nucleotide sequence of a yeast Ty element: evidence for an unusual mechanism of gene expression. Proc Natl Acad Sci 82: 2829–2833 (1985).
Devereux J, Haeberli P, Smithies O: A comprehensive set of sequence analysis programs for the VAX. Nucl Acids Res 12: 387–395 (1984).
Flavell RB: Repetitive DNA and chromosome evolution in plants. Phil Trans R Soc Lond (Biol) 312: 227–242 (1986).
Forde J, Malipica J-M, Halford NG, Shewry PR: The nucleotide sequence of a HMW glutenin subunit gene located on chromosome 1A of wheat (Triticum aestivum). Nucl Acids Res 13: 6817–6832 (1985).
Fourcade-Perronet F, d'Auriol L, Becker J, Bailibert F, Best-Belpomme M: Primary structure and functional organization of Drosophila 1731 retrotransposon. Nucl Acids Res 16: 6113–6125 (1988).
Ghosh HP, Ghosh K, Simsek M, Rajbhandary UL: Nucleotide sequence of wheat germ cytoplasmic initiator methionine transfer ribonucleic acid. Nucl Acids Res 10: 3241–3247 (1982).
Grandbastein MA, Spielman A, Caboche M: Tnt1, a mobile retroviral-like transposable element of tobacco isolated by plant genetics. Nature 337: 376–380 (1989).
Harberd NP, Flavell RB, Thompson RD: Identification of a transposon-like insertion in a Glu-1 allele of wheat. Mol Gen Genet 209: 326–332 (1987).
Harris N: A Transposable Element of Wheat. Ph.D. thesis, Cambridge (1987).
Henikoff S: Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28: 351–359 (1984).
Higgins DG, Sharp PM: CLUSTAL: a package for performing multiple sequence alignments on a microcomputer. Gene 73: 237–244 (1988).
Johns MA, Mottinger J, Freeling M: A low copy number copia-like transposon in maize. EMBO J 4: 1093–1102 (1985).
Johns MA, Babcock MA, Fuerstenberg SM, Fuerstenberg SI, Freeling M, Simpson RB: Ah unusually compact retrotransposon in maize. Plant Mol Biol 12: 633–642 (1989).
Lucas H, Moore G, Murphy GJP, Flavell RB: The nucleotide sequence of the wheat WIS-2-1A retrotransposon like elements LTRs suggests that their large size is partly due to palindromic structures. Mol Biol Evol, in press (1991).
Moore G, Lucas H, Batty N, Flavell RB: A family of retrotransposons and associated variations in wheat. Genomics 10: 461–468 (1991).
Moore G, Cheung W, Schwarzacher T, Flavell R: BIS 1, a major component of the cereal genome and a tool for studying genomic organization. Genomics 10: 469–476 (1991).
Mount SM, Rubin GM: Complete nucleotide sequence of the Drosophila transposable element copia: homology between copia and retroviral proteins. Mol Cell Biol 5: 1630–1638 (1985).
Murphy GJP, Ward ES: Sequencing of double-stranded DNA. In: Howe CJ, Ward ES (eds) Nucleic Acids Sequencing: A Practical Approach, pp 99–115. IRL Press, Oxford (1990).
Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5468 (1977).
Sentry JW, Smyth DR: An element with long terminal repeats and its variant arrangements in the genome of Lilium henryi, Mol Gen Genet 215: 349–354 (1989).
Staden R: The current status and portability of our sequence handling software. Nucl Acids Res 14: 217–231 (1986).
Voytas DF, Ausubel FM: A copia-like transposable element family in Arabidopsis thaliana. Nature 336: 242–244 (1988).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Murphy, G.J.P., Lucas, H., Moore, G. et al. Sequence analysis of WIS-2-1A, a retrotransposon-like element from wheat. Plant Mol Biol 20, 991–995 (1992). https://doi.org/10.1007/BF00027169
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00027169