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Presence of an N-terminal presequence in the PsaI protein of the Photosystem I complex in the filamentous cyanobacterium Anabaena variabilis ATCC 29413

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Abstract

The psaI gene encoding the 5.2 kDa protein component (PsaI) of the photosystem I complex was cloned from the cyanobacterium Anabaena 29413. The gene is present in single copy in this cyanobacterial genome. The nucleotide sequence of a 500 bp region of the cloned DNA revealed the presence of an open reading frame encoding a 46 amino acid long polypeptide. The N-terminal 11 residues are absent in the mature polypeptide and thus represents the first identified cleavable presequence on the PsaI protein. We suggest that this presequence directs the N-terminus of the protein to the thylakoid lumen.

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References

  1. Allen MM: Simple condition for the growth of unicellular blue-green algae on plates. J Phycol 4: 1–4 (1968).

    Google Scholar 

  2. Golbeck JH, Bryant DA: Photosystem I. In: Lee CP (ed) Current Topics in Bioenergetics, vol. 16, pp. 83–177. Academic Press, New York (1991).

    Google Scholar 

  3. Golden SS, Brusslan JA, Haselkorn R: Genetic engineering of the cyanobacterial chromosome. Meth Enzymol 153: 215–231 (1987).

    Google Scholar 

  4. Ikeuchi M, Hirano A, Inoue Y: Polypeptide composition of higher plant photosystem I complex. Identification of psaI, psaJ, psaK gene products. FEBS Lett 263: 274–278 (1990).

    Google Scholar 

  5. Ikeuchi M, Nyhus KJ, Inoue Y, Pakrasi HB: Identities of four low-molecular-mass subunits of the photosystem I complex from Anabaena variabilis ATCC 29413. FEBS Lett 287: 5–9 (1991).

    Google Scholar 

  6. Koike H, Mamada K, Ikeuchi M, Inoue Y: Low-molecular-mass proteins in cyanobacterial photosystem II: identification of psbH and psbK gene products by N-terminal sequencing. FEBS Lett 244: 391–396 (1989).

    Google Scholar 

  7. Maldener I, Lockau W, Cai Y, Wolk CP: The calcium-dependent protease of the cyanobacterium Anabaena: Molecular cloning and expression of the gene in Escherichia coli and site directed mutagenesis. Mol Gen Genet 225: 113–120 (1991).

    Google Scholar 

  8. Mannan RM, Whitmarsh J, Nyman P, Pakrasi HB: Directed mutagenesis of an iron sulfur-protein of the photosystem I complex in the filamentous cyanobacterium Anabaena variabilis ATCC 29413. Proc Natl Acad Sci USA 88: 10168–10172 (1991).

    Google Scholar 

  9. Mannan RM, Pakrasi HB: Molecular analysis of the psaC gene encoding the FA/FB apoprotein of Photosystem I in the filamentous cyanobacterium Anabaena variabilis ATCC 29413. Plant Physiol 98: 798–800 (1992).

    Google Scholar 

  10. Nyhus KJ, Ikeuchi M, Inoue Y, Whitmarsh J, Pakrasi HB: Purification and characterization of the Photosystem I complex from the filamentous cyanobacterium Anabaena variabilis ATCC 29413. J Biol Chem 267: 12489–12495 (1992).

    Google Scholar 

  11. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).

    Google Scholar 

  12. Scheller HV, Okkels JS, Hoj PB, Svendsen I, Roepstorff P, Moller BL: The primary structure of a 4.0-kDa Photosystem I polypeptide encoded by the chloroplast psaI gene. J Biol Chem 264: 18402–18406 (1989).

    Google Scholar 

  13. van derPlas J, Bovy A, Kruyt F, deVrieze G, Dassen E, Klein B, Weisbeek P: The gene for the precursor of plastocyanin from the cyanobacterium Anabaena sp. PCC 7937: isolation, sequence and regulation. Mol Microbiol 3: 275–284 (1989).

    Google Scholar 

  14. vonHeijne G, Gavel Y: Topogenic signals in integral membrane proteins. Eur J Biochem 174: 671–678 (1988).

    Google Scholar 

  15. Wolk CP, Shaffer PW: Heterotrophic micro- and macrocultures of a nitrogen-fixing cyanobacterium. Arch Microbiol 110: 145–147 (1976).

    Google Scholar 

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Sonoike, K., Ikeuchi, M. & Pakrasi, H.B. Presence of an N-terminal presequence in the PsaI protein of the Photosystem I complex in the filamentous cyanobacterium Anabaena variabilis ATCC 29413. Plant Mol Biol 20, 987–990 (1992). https://doi.org/10.1007/BF00027168

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  • DOI: https://doi.org/10.1007/BF00027168

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