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An mRNA that specifically accumulates in maize roots delineates a novel subset of developing cortical cells

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Abstract

A near full-length cDNA clone (pZRP3) corresponding to an mRNA that accumulates specifically in roots of maize was isolated. The ZRP3 mRNA is ca. 600 nucleotides in length. The amino acid sequence of the predicted polypeptide is rich in leucine (16%), proline (11%), and cysteine (8.5%). The zrp3 gene appears to be expressed exclusively in roots, whereas other ZRP3-related genes are expressed in additional organs of the maize plant. In situ hybridization shows that ZRP3 mRNA accumulation is largely confined to the cells of the cortical ground meristem. Furthermore, accumulation of this mRNA occurs within a distinct subset of cortical cells, the inner three to four cell layers.

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Author notes

  1. Isaac John

    Present address: Department of Physiology and Environmental Science, University of Nottingham School of Agriculture, Sutton Bonington, LE12 5RD, Loughborough, Leicestershire, UK

Authors and Affiliations

  1. Department of Botany, Iowa State University, 50011-1020, Ames, Iowa, USA

    Isaac John, Huiqing Wang, Bruce M. Held, Eve Syrkin Wurtele & James T. Colbert

Authors
  1. Isaac John
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  2. Huiqing Wang
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  3. Bruce M. Held
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  4. Eve Syrkin Wurtele
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  5. James T. Colbert
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Additional information

Journal paper number J-14572 of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa Project Number 2997.

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John, I., Wang, H., Held, B.M. et al. An mRNA that specifically accumulates in maize roots delineates a novel subset of developing cortical cells. Plant Mol Biol 20, 821–831 (1992). https://doi.org/10.1007/BF00027153

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  • DOI: https://doi.org/10.1007/BF00027153

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