Skip to main content
Log in

Discharge of nematocysts isolated from aeolid nudibranchs

  • Proceedings
  • XIII. Nematocysts
  • Published:
Hydrobiologia Aims and scope Submit manuscript

Abstract

Nematocysts were extracted from 3 nudibranch species and one sea anemone species, and the ability of several test fluids to promote discharge was examined. Except when isolated in sodium citrate, nudibranch nematocysts did not discharge in response to any test fluids. Nudibranch nematocysts isolated in sodium citrate discharged when tested with EGTA, distilled water, and calcium-free artificial seawater, but there were differences among the 3 nudibranch species. Nematocysts isolated from one nudibranch species and nematocysts isolated from that nudibranch's sea anemone prey differed in the percentage that discharged in response to EGTA and distilled water. These results suggest that nematocysts stored by nudibranchs are altered in some way, resulting in the different discharge responses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Blanquet R. 1970. Ionic effects on discharge of the isolated and in situ nematocysts of the sea anemone Aiptasia pallida: a possible role of calcium. Comp. Biochem. Physiol. 35: 451–461.

    Google Scholar 

  • Conklin, E. J. & R. N. Mariscal, 1977. Feeding behavior, ceras structure, and nematocyst storage in the aeolid nudibranch, Spurilla neapolitana (Mollusca). Bull. mar. Sci. 27: 658–667.

    Google Scholar 

  • Day, R. M. & L. G. Harris, 1978. Selection and turnover of coelenterate nematocysts in some aeolid nudibranchs. Veliger 21: 104–109.

    Google Scholar 

  • Greenwood, P. G. 1987. Nematocyst maintenance and orientation in aeolid nudibranchs. Ph.D. thesis, Florida State Univ., Tallahassee.

    Google Scholar 

  • Greenwood, P. G. 1988. Nudibranch nematocysts. In D. A. Hessinger & H. M. Lenhoff (eds). The Biology of Nematocysts. Academic Press, N.Y.: 445–462.

    Google Scholar 

  • Greenwood, P. G. & R. N. Mariscal, 1984. The utilization of cnidarian nematocysts by aeolid nudibranchs: nematocyst maintenance and release in Spurilla. Tissue Cell 16: 719–730.

    Google Scholar 

  • Gupta, B. C. & T. A. Hall, 1984. Role of high concentration of Ca, Cu, and Zn in the maturation and discharge in situ of sea anemone nematocysts as shown by x-ray microanalysis of cryosections. In L. Bolis, J. Zadunaisky & R. Gilles (eds), Toxins, Drugs and Pollutants in Marine Animals. Springer-Verlag, Berlin: 77–95.

    Google Scholar 

  • Harris, L. G., 1973. Nudibranch associations. In T. C. Cheng (ed.), Current Topics in Comparative Pathobiology V. II. Academic Press, N.Y.: 213–315.

    Google Scholar 

  • Hidaka, M. & R. N. Mariscal, 1988. Effects of ions on nematocysts isolated from acontia of the sea anemone Calliactis tricolor by different methods. J. exp. Biol. 136: 23–34.

    Google Scholar 

  • Lubbock, R. & W. B. Amos, 1981. Removal of bound calcium from nematocyst contents causes discharge. Nature, Lond. 290: 500–501.

    Google Scholar 

  • Lubbock, R., B. L. Gupta & T. A. Hall, 1981. Novel role of calcium in exocytosis: Mechanisms of nematocyst discharge as shown by X-ray microanalysis. Proc. nam. Acad. Sci. U.S.A. 78: 3624–3628.

    Google Scholar 

  • Mariscal, R. N. 1988. X-ray microanalysis and perspectives on the role of calcium and other elements in cnidae. In D. A. Hessinger & H. M. Lenhoff (eds), The Biology of Nematocysts. Academic Press, N.Y.: 95–113.

    Google Scholar 

  • McKay, M. C. & P. A. V. Anderson, 1988. On the preparation and properties of isolated cnidocytes and cnidae. In D. A. Hessinger & H. M. Lenhoff (eds), The Biology of Nematocysts. Academic Press, N.Y.: 273–293.

    Google Scholar 

  • Salleo, A., G. La Spada & M. Alfa, 1983. Blockage of trypsininduced discharge of nematocysts of Pelagia noctiluca by Ca2+. Molec. Physiol. 3: 89–97.

    Google Scholar 

  • Salleo, A., G. La Spada & M. G. Denaro, 1988. Release of free Ca2+ from the nematocysts of Aiptasia mutabilis during the discharge. Physiol. Zool. 61: 272–279.

    Google Scholar 

  • Salleo, A., G. La Spada, G. Falzea & M. G. Denaro, 1984. Discharging effect of anions and inhibitory effect of divalent cations on isolated nematocysts of Pelagia noctiluca. Molec. Physiol. 5: 25–34.

    Google Scholar 

  • Tardent, P. 1988. History and current state of knowledge concerning discharge of cnidae. In D. A. Hessinger & H. M. Lenhoff (eds), The Biology of Nematocysts. Academic Press, N.Y.: 309–332.

    Google Scholar 

  • Watson, G. M.& D. A. Hessinger, 1989a. Cnidocyte mechanoreceptors are tuned to the movements of swimming prey by chemoreceptors. Science, N.Y. 243: 1589–1591.

    Google Scholar 

  • Watson, G. M. & D. A. Hessinger, 1989b. Cnidocytes and adjacent supporting cells form receptor-effector complexes in anemone tentacles. Tissue Cell 21: 17–24.

    Google Scholar 

  • Weber, J., M. Klug & P. Tardent, 1987. Some physical and chemical properties of purified nematocysts of Hydra attenuata Pall. (Hydrozoa, Cnidaria). Comp. Biochem. Physiol. 88B: 855–862.

    Google Scholar 

  • Zierold, K., I. Gerke & M. Schmitz, 1989. X-ray microanalysis of fast exocytotic processes. In K. Zierold & H. K. Hagler (eds), Electron Probe Microanalysis: Applications in Biology and Medicine. Springer-Verlag, Berlin: 281–292.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Greenwood, P.G., Garrity, L.K. Discharge of nematocysts isolated from aeolid nudibranchs. Hydrobiologia 216, 671–677 (1991). https://doi.org/10.1007/BF00026529

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00026529

Key words

Navigation