Abstract
The capsules of isolated mastigophores of C. fleckeri were impermeable to water and neutral red dye. After air drying, ca 30% discharged, most everting fully, when exposed to distilled water or sodium citrate but in a seawater medium only partial discharge was induced. The capsular contents of discharging mastigophores dyed strongly red with neutral red and showed metachromasy with toluidine blue. Electron micrographs revealed hexagonal arrays of granules ca 12 nm in diam. Evidence supports the view that a polymerization of the capsular material initiates tubule eversion and that complete eversion involves osmotic inflow of water and sustained compression of the capsular contents.
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References
Daddow, L. Y. M., 1986. An abbreviated method of double lead stain technique. J. submicrosc. Cytol. 18: 221–224.
Endean, R., 1987. Separation of two myotoxins from nematocysts of the box jellyfish (Chironex fleckeri). Toxicon 25: 483–492.
Endean, R. & J. F. Rifkin, 1983. Envenomation involving nematocysts of the box jellyfish Chironex fleckeri. Toxicon Suppl. 3: 115–118.
Endean, R., C. Duchemin, D. McCohn & E. H. Fraser, 1969. A study of the biological activity of toxic material derived from nematocysts of the cubomedusan Chironex fleckeri. Toxicon 6: 179–204.
Godknecht, A. & P. Tardent, 1988. Discharge and mode of action of the tentacular nematocysts of Anemonia sulcata (Anthozoa: Cnidaria). Mar. Biol. 100: 83–92.
Lubbock, R. & W. B. Amos, 1981. Removal of bound calcium from nematocyst contents causes discharge. Nature, Lond. 290: 500–501.
Mariscal, R. N., 1974. Nematocysts. In L. Muscatine & H. M. Lenhoff (eds), Coelenterate Biology: Reviews and New Perspectives. Academic Press, N.Y.: 129–178.
Pearse, A. G. E., 1968. Histochemistry Theoretical and Applied, 1. 3rd edn J. & A. Churchill, Lond., 759 pp.
Rifkin, J. F. & R. Endean, 1983. The structure and function of the nematocysts of Chironex fleckeri Southcott, 1956. Cell Tissue Res. 233: 563–577.
Rifkin, J. F. & R. Endean, 1988. Arrangement of accessory cells and nematocytes bearing mastigophores in the tentacles of the cubozoan Chironex fleckeri. J. Morph. 195: 103–115.
Tardent, P., T. Holstein, J. Weber & M. Klug, 1985. The morphodynamics and actions of stenotele nematocysts in Hydra. Archs Sci. Genève 38: 401–418.
Weill, R., 1934. Contribution à l'étude des cnidaires et de leurs nématocytes. I. Trav. Stn zool. Wimereux 10: 1–347.
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Endean, R., Rifkin, J.F. & Daddow, L.Y.M. Envenomation by the box-jellyfish Chironex fleckeri: how nematocysts discharge. Hydrobiologia 216, 641–648 (1991). https://doi.org/10.1007/BF00026525
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DOI: https://doi.org/10.1007/BF00026525