Abstract
Isolated striated muscle of the anthomedusa of Podocoryne carnea can be stimulated to carry out DNA synthesis and transdifferentiation to RF-amide positive nerve cells by the protein kinase C-activating drugs 12-O-tetradecanoyl-13-acetate (TPA), mezerein and diacylglycerol. Methylether-TPA, which does not activate protein kinase C, is unable to induce transdifferentiation. In isolated muscle tissue treated simultaneously with TPA and K-252a, an inhibitor of protein kinase C, the rate of transdifferentiation is greatly reduced. The role of protein kinase C in transdifferentiation is discussed.
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Alder, H. & V. Schmid, 1987. Cell cycles and in vitro transdifferentiation and regeneration of isolated, striated muscle of jellyfish. Devl Biol. 124: 358–369.
Blumberg, P. M., 1988. Protein kinase C as the receptor for the phorbol ester tumor promoters. Cancer Res. 48: 1–8.
Castagna, M., 1987. Phorbol esters as signal transducers and tumor promoters. Biol. cell. 59: 3–14.
Castagna, M., Y. Takai, K. Kaibuchi, K. Sano, U. Kikkawa & Y. Nishizuka, 1982. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumorpromoting phorbol esters. J. biol. Chem. 257: 7847–7851.
Claycomb, W. C. & R. L. Moses, 1988. Growth factors and TPA stimulate DNA synthesis and alter the morphology of cultured terminally differentiated adult rat cardiac muscle cells. Devl Biol. 127: 257–265.
Cossu, G., M. Pacifici, S. Adamo, M. Bouche & M. Molinaro, 1982. TPA-induced inhibition of the expression of differentiated traits in cultured myotubes: dependence on protein synthesis. Differentiation 21: 62–65.
Couturier, A., S. Bazgar & M. Castagna, 1984. Further characterization of tumor-promoter-mediated activation of protein kinase C. Biochem. biophys. Res. Communs 121: 448–455.
Croop, J., G. Dubiak, T. Toyama, A. Dlugosz, A. Scarpa & H. Holtzer, 1982. Effects of 12-O-tetradecanoyl-phorbol13-acetate on myofibril integrity and Ca2+ content in developing myotubes. Devl Biol. 89: 460–474.
Dlugosz, A. A., S. J. Tapscott & H. Holtzer, 1983. Effects of phorbol 12-myristate 13-acetate on the differentiation program of embryonic chick skeletal myoblasts. Cancer Res. 43: 2780–2789.
Doctrow, S. R. & J. Folkman, 1987. Protein kinase C activators suppress stimulation of capillary endothelial cell growth by angiogenic endothelial mitogens. J. Cell Biol. 104: 679–687.
Glimelius, G. & J. Weston, 1981. Analysis of developmentally homogeneous neural crest cell populations. II A tumor promoter (TPA) delays differentiation and promotes cell proliferation. Devl Biol. 82: 95–101.
Grimmelikhuijzen, C. P. J., 1983. FMRFamide immunoreactivity is generally occurring in the nervous system of coelenterates. Histochemistry 78: 361–381.
Huang, C. L. & H. E. Ives, 1987. Growth inhibition by protein kinase C late in mitogenesis. Nature, Lond. 329: 849–850.
Kase, H., K. Iwahashi, Y. Matsuda, K. Yamada, M. Takahashi, C. Murakata, A. Sato & M. Kaneka, 1987. K-252 compounds, novel and potent inhibitors of protein kinase C and cyclic nucleotide-dependent protein kinases. Biochem. biophys. Res. Communs 142: 436–440.
Kikkawa, U. & Y. Nishizuka, 1986. The role of protein kinase C in transmembrane signalling. A. Rev. Cell Biol. 2: 149–178.
Lin, Z., J. Eshleman, C. Grund, D. A. Fischman, T. Masaki, W. W. Franke & H. Holtzer, 1989. Differential response of myofibrillar and cytoskeletal proteins in cells treated with phorbol myristate acetate. J. Cell Biol. 108: 1079–1091.
Miyake, R., Y. Tanaka, T. Tsuda, K. Kaibuchi, U. Kikkawa & Y. Nishizuka, 1984. Activation of protein kinase C by non-phorbol tumor promoter, mezerein. Biochem. biophys. Res. Communs 121: 649–656.
Montesano, R. & L. Orci, 1985. Tumor-promoting phorbol esters induced angiogenesis in vitro. Cell 42: 469–477.
Niedel, J. E., L. J. Kuhn & G. R. Vandenbark, 1983. Phorbol diester receptor copurifies with protein kinase C. Proc. natn. Acad. Sci. U.S.A. 80: 36–40.
Nishizuka, Y., 1984. The role of protein kinase C in cell surface signal transduction and tumour promotion. Nature, Lond. 308: 693–698.
Nishizuka, Y., 1986. Studies and perspectives of protein kinase C. Science, N.Y. 233: 305–312.
Nishizuka, Y., 1988. The molecular heterogeneity of protein kinase C and its implication for cellular regulation. Nature, Lond. 334: 661–665.
Okada, T. S., 1983. Recent progress in studies of the transdifferentiation of eye tissues in vitro. Cell Diff. 13: 177–183.
Rozengurt, E., 1986. Early events in the mitogenic response. Science, N.Y. 234: 161–166.
Schmid, V., 1971. Untersuchungen über Dedifferenzierungsvorgänge bei Medusenknospen and Medusen von Podocoryne carnea M. Sars. Ph.D. thesis, University of Zurich.
Schmid, V., 1978. Striated muscle: influence of an acellular layer on the maintenance of muscle differentiation in anthomedusae. Devl Biol. 64: 48–59.
Schmid, V., 1979. The use of an anthomedusa in establishing an in vitro regeneration system. Annls Soc. fr. Biol. Dev. 179: 35–38.
Schmid, V., 1988. The potential for transdifferentiation and regeneration of isolated striated muscle of medusae in vitro. Cell Diff. 22: 173–182.
Schmid, V. & H. Alder, 1984. Isolated, mononucleated, striated muscle can undergo pluripotent transdifferentiation and form a complex regenerate. Cell 38: 801–809.
Schmid, V., C. Weber & D. Keller, 1983. Factors affecting DNA synthesis in an in vitro system. Wilhelm Roux Arch. dev. Biol. 193: 36–41.
West, C. M. & H. Holtzer, 1982. Protein synthesis and degradation in cultured muscle is altered by a phorbol diester tumor promoter. Archs Biochem. Biophys. 219: 335–350.
Yamada, K., 1977. Control mechanisms in cell-type conversions in newt lens regeneration. In A. Awalsky (ed.), Monographs in Developmental Biology. S. Karger, Basel: 13.
Yamada, K., K. Iwahashi & H. Kase, 1987. K252-a, a new inhibitor of protein kinase C, concomitantly inhibits 40K protein phosphorylation and serotonin secretion in phorbol ester stimulated platelets. Biochem. biophys. Res. Communs 144: 35–40.
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Kurz, E., Schmid, V. Effects of tumor promoters and diacylglycerol on the transdifferentiation of striated muscle cells of the medusa Podocoryne carnea to RF-amide positive nerve cells. Hydrobiologia 216, 11–17 (1991). https://doi.org/10.1007/BF00026437
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DOI: https://doi.org/10.1007/BF00026437