Summary
Cross-incompatibility between cultivated peanuts and their wild relatives outside the section Arachis has impeded the utilization of many species possessing high resistances or good qualities. Despite the great efforts made to culture immature ovules or embryos, few hybrid offspring have been obtained. In this study, gynophores from Arachis hypogaea L. pollinated with A. glabrata Benth. were cultured and F1 hybrids seeds were harvested, and F2 and F3 generations produced. The characters of F2 generation exhibited a wide range of segregation. Leaf peroxidase isozyme PAGE analysis revealed that the hybrids were quite different from their parents in relation to band number, width and isozyme activity. The zymograms of the hybrids and their parents were partially alike. This verified the authenticity of the hybrids.
Similar content being viewed by others
References
Feng, H.S. & F.Y.Shen, 1980. A short communication on peanut gynophore culture. Oil Crops of China 2: 69–72.
Gregory, M.P. & W.C.Gregory, 1979. Exotic germplasm of Arachis L. interspecific hybrids. The Journal of Heredity 70: 185–193.
Halward, T.M. & H.T.Stalker, 1987. Incompatibility mechanism in interspecific peanut hybrids. Crop Sci. 1987, 456–560.
Hull, F.H. & W.A. Carver, 1938. Peanut Improvement, Florida Agric. Exp. Sta. Annual Report 1938, 39–40.
ICRISAT, 1985. Cytogenetics of Arachis. p. 153–158.
ICRISAT, 1989. Annual Report, 1982–1989.
ICRISAT, 1990. Legumes Program Annual Report. p. 93–94.
Moss, J.P. & H.T.Stalker, 1987. Embryo rescue in wide crosses in Arachis. 3. In vitro culture of peg tips of A. hypogaea selfs and interspecific hybrids. Peanut Science 14: 70–74.
Moss, J.P., A.K. Singh, D.C. Sastri & I.S. Dundas, 1988. Wide hybridization in legumes at ICRISAT. p. 90–93. In: J.M.J. de Wet & T.A. Preston (Eds). Biotechnology in Tropical Crop Improvement. ICRISAT.
Moss, J.P., H.T.Stalker & H.E.Pattee, 1988. Embryo rescue in wide crosses in Arachis. 1. Culture of ovules in peg tips of Arachis hypogaea. Annals of Botany 61: 1–7.
Pattee, H.E., H.T.Stalker & J.P.Moss, 1988. Embryo rescue in wide crosses in Arachis. 2. Embryo development in cultured peg tips of Arachis hypogaea. Annals of Botany 61: 103–112.
Sastri, D.C., J.P. Moss & M. Nalini, 1982. The use of in vitro techniques in groundnut improvement. In: L. Kenneth & S.K. Sen (Eds). Plant Cell Culture in Crop Improvement. Plenum Publishing Corporation.
Shen, F.Y. & Z.X.Wang, 1983. A study on cross-compatibility between cultivated peanuts and their wild relatives. Peanut Science and Technology 2: 28–30.
Shen, F.Y., Z.X.Wang & X.M.Gan, 1984. A study of the somatic doubling technique in peanut interspecific triploid hybrids. Scientia Agricultura Sinica 4: 21–25.
Shen, F.Y., 1990. A short communication on culture of gynophores from cultivated peanuts by A. glabrata. Peanut Science and Technology 1: 10–11.
Smartt, J. & W.C.Gregory, 1967. Interspecific cross-compatibility between the cultivated peanut Arachis hypogaea L. and other members of the genus Arachis. Oleagineux 7: 1–5.
Wang, C.T. & F.Y.Shen, 1991. The progress in researching incompatible obstacle and tackling method in peanut wide crosses. Scientific & Technical Information 3: 22–23.
Ziv, M. & E.Zamski, 1975. Geotropic responses and pod development in gynophore explants of peanut (Arachis hypogaea) cultured in vitro. Annals of Botany 39: 579–583.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shen, F.Y., Wang, C.T. & Duan, S.F. Aseptic culture of gynophores to obtain peanut intersectional hybrids. Euphytica 81, 245–249 (1995). https://doi.org/10.1007/BF00025613
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00025613