Advertisement

Plant Growth Regulation

, Volume 8, Issue 3, pp 215–223 | Cite as

Identification of indole-3-butyric acid as an endogenous constituent of maize kernels and leaves

  • Ephraim Epstein
  • Kai-Hsien Chen
  • Jerry D. Cohen
Article

Abstract

Indole-3-butyric acid (IBA) was identified by thin layer chromatography, gas-liquid chromatography and gas chromatography-mass spectrometry in kernels and leaves of corn (Zea mays) var. Hazera 224. Free and ester conjugated IBA were present in dry and germinating corn kernels and leaves. This is the first report of IBA in a monocotyledonous plant and, as far as we know, the first evidence for the presence of conjugated IBA.

Key words

IAA IBA conjugated IBA rooting Zea mays 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Andreae WA and Good NE (1957) Studies on 3-indoleacetic acid metabolism. IV. Conjugation with aspartic acid and ammonia as process in the metabolism of carboxylic acids. Plant Physiol. 32: 566–571Google Scholar
  2. 2.
    Badenoch-Jones J, Summons RE, Rolfe BG and Letham DS (1984) Phytohormones, Rhizobium mutants and nodulation in legumes. IV. Auxin metabolites in pea root nodules. J. Plant Growth Regulators 3: 23–29.Google Scholar
  3. 3.
    Bandurski RS and Schulze Aga (1977) Concentration of indole-3-acetic acid and its derivatives in plants. Plant Physiol. 60: 211–213Google Scholar
  4. 4.
    Bayer MH (1969) Gas chromatographic analysis of acidic indole auxins in Nicotiana. Plant Physiol. 44: 267–271PubMedGoogle Scholar
  5. 5.
    Blommaert KLJ (1954) Growth- and inhibiting-substances in relation to the rest period of the potato tuber. Nature 174: 970–972.PubMedGoogle Scholar
  6. 6.
    Chen K-H, Miller AN, Patterson GW and Cohen JD (1987) A rapid and simple procedure for purification of indole-3-acetic acid prior to GC-SIM-MS analysis. Plant Physiol 86: 822–825Google Scholar
  7. 7.
    Chen K-H (1987) Analysis of indole-3-acetic acid in tobacco genetic tumors and wheat GA3 insensitive mutant "Tom Thumb" Ph.D. Thesis. University of MarylandGoogle Scholar
  8. 8.
    Cohen JD (1984) Convenient apparatus for the generation of small amounts of diazomethane. J. Chromatogr. 303: 193–196CrossRefGoogle Scholar
  9. 9.
    Ehmann A (1977) The Van-Urk-Salkowski reagent—a sensitive and specific chromogenic reagent for silica gel thin layer detection and identification of indole derivatives. J. Chromatogr. 132: 267–276CrossRefPubMedGoogle Scholar
  10. 10.
    Epstein E and Lavee S (1984) Conversion of indole-3-butyric acid to indole-3-acetic acid by cuttings of grapevine (Vitis vinifera) and olive (Olea europeae). Plant & Cell Physiol. 25: 697–703Google Scholar
  11. 11.
    Epstein E, Muszkat Lea and Cohen JD (1988) Identification of indole-3-butyric acid (IBA) in leaves of cypress and maize by gas chromatography-mass spectrometry (GC/MS). Alon Hanote 42: 917–919Google Scholar
  12. 12.
    Epstein E and Wiesman Z (1987) Improved vegetative propagation of olive cuttings with IBA-alanine. Olea 18: 35–38Google Scholar
  13. 13.
    Fawcett CH, Wain RL and Wightman F (1960) The metabolism of indolylalkenecarboxylic acids and their amides, nitriles and methyl esters in plant tissues. Proc. Royal Soc. London Ser. B. 152: 231–254Google Scholar
  14. 14.
    Nickell LG (1982) Plant Growth Regulators: Agricultural Uses. Berlin: Springer VerlagGoogle Scholar
  15. 15.
    Schneider Elnora A, Kazakoff CW and Wightman F (1985) Gas chromatography-mass spectrometry evidence for several endogenous auxins in pea seedlings organs. Planta 165: 232–241Google Scholar
  16. 16.
    Weaver RJ (1972) Plant Growth Substances in Agriculture. San Francisco: W.H. Freeman and Co.Google Scholar
  17. 17.
    Went FW and Thimann KV (1937) Phytohormones. NY: MacmillanGoogle Scholar
  18. 18.
    Wiesman Z and Epstein E (1987) Metabolism and transport of 3H-indole-3-butyric acid in cuttings of olive. Olea 18: 29–33Google Scholar
  19. 19.
    Wiesman Z, Epstein E and Riov J (1988) Comparison of movement and rate of metabolism of indole-3-acetic acid and indole-3-butyric acid in mung bean cuttings. Physiol Plant. 74: 556–560Google Scholar

Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • Ephraim Epstein
    • 1
  • Kai-Hsien Chen
    • 2
  • Jerry D. Cohen
    • 3
  1. 1.ARO, The Volcani CenterBet DaganIsrael
  2. 2.Department of BotanyUniversity of MarylandCollege ParkUSA
  3. 3.USDA-ARS Plant Hormone LaboratoryBeltsville Agricultural Research CenterBeltsvilleUSA

Personalised recommendations