Summary
Granule-bound starch synthase (GBSS) catalyses the synthesis of amylose in starch granules. Analysis of antisense RNA mediated inhibition of GBSS gene expression in large numbers of tubers from in vitro grown, greenhouse grown and field grown transgenic potato plants revealed stable and total inhibition of GBSS gene expression in one clone. In three other transgenic genotypes partial and unstable inhibition was found. In these genotypes both GBSS activity and amylose content were remarkably reduced compared with the non-transformed control genotype. No relationship was found between the level of inhibition of GBSS gene expression and yield and dry matter content.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anonymous, 1990. Richtlijnen voor de vervaardiging van en handelingen met genetisch gemodificeerde organismen. Issued by: Voorlopige Commissie Genetische Modificatie. pp 1–59.
Hovenkamp-Hermelink, J.H.M., E., Jacobsen, L.P., Pijnacker, J.N.de, Vries, B., Witholt & W.J., Feenstra, 1988a. Cytological studies on adventitious shoots and minitubers of a monoploid potato clone. Euphytica 39: 213–219.
Hovenkamp-Hermelink, J.H.M., J.N.de, Vries, P., Adamse, E., Jacobsen, B., Witholt & W.J., Feenstra, 1988b. Rapid estimation of the amylose/amylopectin ratio in small amounts of tuber and leaf tissue of the potato. Potato Res. 31: 241–246.
Jacobsen, E., J.H.M., Hovenkamp-Hermelink, H.T., Krijgsheld, H., Nijdam, L.P., Pijnacker, B., Witholt & W.J., Feenstra, 1989. Phenotypic and genotypic characterization of an amylose-free starch mutant of potato. Euphytica 44: 43–48.
Kim, S.K. & B.J., Wold, 1985. Stable reduction of thymidine kinase activity in cells expressing high levels of antisense RNA. Cell 42: 129–138.
van der, Krol, A.R., P.E., Lenting, J., Veenstra, I.M.van der, Meer, R.E., Kroes, A.G.M., Gerats, J.N.M., Mol & A.R., Stuitje, 1988. Expression of an antisense chalcone synthase gene in transgenic plants inhibits flower pigmentation. Nature 333: 866–869.
van der, Krol, A.R., J.N.M., Mol & A.E., Stuitje, 1989. Modulation of eukaryotic gene expression by complementary RNA or DNA sequences. Biotechniques 6: 958–976.
van der, Krol, A.R., L.A., Mur, P.de, Lange, A.G.M., Gerats, J.N.M., Mol & A.R., Stuitje, 1990. Antisense chalcone synthase genes in petunia: Visualization of variable transgene expression. Mol. Gen. Genet 220: 204–212.
Murashige, T. & F., Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497.
Ooms, G., M.E., Bossen, M.M., Burrell & A., Karp, 1986. Genetic manipulation in potato with Agrobacterium rhizogenes. Potato Res 29: 367–379.
Shannon, J.C. & D.L., Garwood, 1984. Genetics and physiology of starch development. In: R.L., Whistler, J.N., Bemiller & E.F., Paschall (Eds.). Starch: chemistry and technology. 2nd edn Academic Press, Orlando.
Smith, C.J.S., C.F., Watson, J., Ray, C.R., Bird, P.C., Morris, W., Schuch & D., Grierson, 1988. Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes. Nature 334: 724–726.
Stockhaus, J., M., Höfer, G., Renger, P., Westhoff, T., Wydrzynski & L., Willmitzer, 1990. Antisense RNA efficiently inhibits formation of the 10 kd polypeptide of photosystem II in transgenic potato plants: analysis of the role of the 10 kd protein. EMBO Journal 9: 3013–3021.
Visser, R.G.F., E., Jacobsen, B., Witholt & W.J., Feenstra, 1989. Efficient transformation of potato (Solanum tuberosum L.) using a binary vector in Agrobacterium rhizogenes. Theor. Appl. Genet. 78: 594–600.
Visser, R.G.F., I., Somhorst, G.J., Kuipers, N.J., Ruys, W.J., Feenstra & E., Jacobsen, 1991a. Inhibition of the expression of the gene for granule-bound starch synthase in potato by antisense constructs. Mol Gen. Genet. 225: 289–296.
Visser, R.G.F., A., Stolte & E., Jacobsen, 1991b. Expression of a chimaeric granule-bound starch synthase-GUS gene in transgenic potato plants. Plant Mol. Biol. 17: 691–699.
Vos-Scheperkeuter, G.H., W.de, Boer, R.G.F., Visser, W.J., Feenstra & B., Witholt, 1986. Identification of granule-bound starch synthase in potato tubers. Plant Physiol. 82: 411–416.
Williamson, J.D., M.E., Hirsch-Wyncott, B.A., Larkins & S.B., Gelvin, 1989. Differential accumulation of a transcript driven by the CaMV 35S promoter in transgenic tobacco. Plant Physiol. 90: 1570–1576.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kuipers, G.J., Vreem, J.T.M., Meyer, H. et al. Field evaluation of antisense RNA mediated inhibition of GBSS gene expression in potato. Euphytica 59, 83–91 (1991). https://doi.org/10.1007/BF00025364
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00025364