Summary
In vitro tillers of over 1000 genotypes from nine contrasting Lolium perenne cultivars were cultured on various media containing the synthetic cytokinin, 6-benzylaminopurine (BAP), in order to determine the effect on tillering. The cultures were assessed after seven weeks for survival, tiller production, size and vigour. Added auxins 2,4-dichlorophenoxyacetic acid (2,4-D) and α-napthaleneacetic acid (NAA) had no consistent beneficial effects.
A significant relationship was found between in vitro tiller production and cultivar heading date. Genotypes of early-heading, less persistent cultivars produced more tillers than genotypes from late heading more persistent cultivars. This was consistent with the generally higher in vitro tiller production in the biennial species Lolium multiflorum cultured on similar media.
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References
Dalton, S.J. & P.J. Dale, 1981. Induced tillering of Lolium multiflorum in vitro. Plant Cell Tissue Organ Culture 1: 57–64.
Dalton, S.J. & P.J. Dale, 1985. The application of in vitro tiller induction in Lolium multiflorum. Euphytica 34: 897–904.
Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant 15: 473–497.
Pollock, C.J., 1982. Patterns of turnover of fructans in leaves of Dactylis glomerata L. New Phytol. 90: 645–650.
Classified List of Herbage Varieties England and Wales 1989/90. National Institute of Agricultural Botany, Cambridge.
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Humphreys, N.C., Dalton, S.J. A relationship between heading date and in vitro tillering propensity in perennial ryegrass (Lolium perenne L.). Euphytica 58, 253–257 (1991). https://doi.org/10.1007/BF00025257
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DOI: https://doi.org/10.1007/BF00025257