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Endogenous cytokinins in rose petals and the effect of exogenously applied cytokinins on flower senescence

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Abstract

In extracts from rose petals cytokinin activity was detected by Amaranthus bioassay in HPLC eluates corresponding to the standards: Z, ZR, 2iP and 2iPA; subsequently, the presence of two groups of endogenous cytokinins was confirmed by ELISA.

Measurements of senesence indicators (cell sap osmolarity and conductivity) and observations of flower vase-life indicated that when the above cytokinins were applied as holding solutions they delayed flower senescence by 34–56% and prolonged rose longevity.

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Abbreviations

B.H.T.:

2.6-di-t-buytl-4-methyl phenol

ELISA:

Enzyme linked Immunosorbent Assay

HPLC:

High Performance Liquid Chromatography

2iP:

isopentenyladenine

2iPA:

isopentenyladenosine

Z:

trans-zeatin

ZR:

trans-zeatin riboside

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Authors and Affiliations

  1. Department of Ornamental Plants, Warsaw Agricultural University, ul. Nowoursynowska 166, 02-766, Warszawa, Pologne

    Alexandra J. Lukaszewska

  2. Laboratoire de Physiologie végétale, Université de Nice - Sophia Antipolis, Parc Varole, 06108, Nice Cedex, France

    Jacqueline Bianco, Philippe Barthe & Marie Thérèse Le Page-Degivry

Authors
  1. Alexandra J. Lukaszewska
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  2. Jacqueline Bianco
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  3. Philippe Barthe
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  4. Marie Thérèse Le Page-Degivry
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Lukaszewska, A.J., Bianco, J., Barthe, P. et al. Endogenous cytokinins in rose petals and the effect of exogenously applied cytokinins on flower senescence. Plant Growth Regul 14, 119–126 (1994). https://doi.org/10.1007/BF00025212

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  • DOI: https://doi.org/10.1007/BF00025212

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