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Auxins, ABA and gibberellin-like activity in abscising and non-abscising flowers and pods of Vicia faba L.

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Abstract

Abscission probability varies among floral positions within inflorescences of Vicia faba L. Flowers from proximal positions have a greater chance to develop into mature pods than flowers from more distal positions which normally abscise either as older flowers or as young pods. In three field experiments with the indeterminate single stem variety ‘Herz-Freya’, changes in the contents of extractable auxins, abscisic acid (ABA) and gibberellins in flowers and pods during their development, and their possible influence on abscission were investigated.

Inflorescences at different positions along the stem were divided into the two proximal and the remaining fruits. The content of all three hormones was at a low level during flower development, increased greatly in parallel with dry matter accumulation in the young pods, and then decreased to maturity. The first hormone to increase in the fruits was auxin and this took place when abscission from the distal positions began. ABA and gibberellins at this time were still at a low level. This ontogenic course of hormone production was very similar in fruits of both positions within an inflorescence, but in flowers and young pods from proximal positions, auxin content in most inflorescences was greater than in those from the abscising distal positions. No such positional differences were observed with ABA and gibberellins. Decapitation of the plants reduced flower and pod drop from the remaining reproductive nodes. Although decapitation resulted in less abscission among distal flowers and young pods from these nodes, it did not affect the ontogenic course of auxin and ABA production in these fruits.

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References

  1. Abeles FB (1973) Ethylene in Plant Biology New York: Academic Press.

    Google Scholar 

  2. Aufhammer W and Zoschke A (1982) Beeinflussung der Beziehungen zwischen den einzelnen Kornanlagen von Weizenähren durch lokale Wirkstoffapplikationen. Z Acker u Pflanzenbau 151: 338–353

    Google Scholar 

  3. Baldev B, Lang A and Agatep AO (1965) Gibberellin production in pea seeds developing in excised pods: Effect of growth retardant AMO-1618. Science 147: 155–157

    PubMed  Google Scholar 

  4. Bellucci S, Keller ER and Schwendimann F (1982) Einfluss von Wachstumsregulatoren auf die Entwicklung und den Ertragsaufbau der Ackerbohne (Vicia faba L.). Teil I: Wirkung von Gibberellinsäure (GA3) auf die Ertragskomponenten und die Versorgung der jungen Früchte mit 14C. Angew Botanik 56: 35–53

    Google Scholar 

  5. Böttger M, Engvild KC and Kaiser P (1978) Response of substituted indoleacetic acids in the indolo-α-pyrone fluorescence determination. Physiol Plant 43: 62–64

    Google Scholar 

  6. Bond DA and Poulsen MH (1983) Pollination. In: PD Hebblethwaite, ed. The Faba Bean (Vicia faba L.), pp. 77–101. London: Butterworth

    Google Scholar 

  7. Chapman GP, Guest HL and Peat WE (1978) Top-removal in single stem plants of Vicia faba L. Z Pflanzenphys 89: 119–127

    Google Scholar 

  8. Clifford PE, Offler CE and Patrick JW (1986) Growth regulators have rapid effects on photosynthate unloading from seed coats of Phaseolus vulgaris L. Plant Physiol 80: 635–637

    Google Scholar 

  9. Coombe BG, Cohen D and Paleg LG (1967) Barley endosperm bioassay for gibberellins. I. Parameters of the response system. II. Application of the method. Plant Physiol 42: 105–119

    Google Scholar 

  10. Diethelm R, Keller ER and Bangerth F (1986) Interactions between the application of growth regulators, yield components and content of phytohormones in the fruits of Vicia faba L. Fabis Newsletter 14: 12–17

    Google Scholar 

  11. Ebert A and Bangerth F (1982) Possible hormonal modes of action of three apple thinning agents. Scientia Horticult 16: 343–356

    Article  Google Scholar 

  12. Eeuwens CJ and Schwabe WW (1975) Seed and pod wall development in Pisum sativum L. in relation to extracted and applied hormones. J Exp Bot 26: 1–14

    Google Scholar 

  13. El-Antably HM (1976) Studies of the physiology of shedding of buds, flowers and fruits of Vicia faba L. I. Effect of cycocel (CCC) and the role of endogenous auxin and abscisic acid. Z Pflanzenphys 80: 21–28

    Google Scholar 

  14. El-Beltagy AS, El-Beltagy MS and Hall MA (1979) Modification of vegetative development, flowering and fruiting behaviour in Vicia faba L., by treatment with growth regulators. Egypt J Hort 6: 237–260

    Google Scholar 

  15. Engvild KC, Egsgaard H and Larsen E (1980) Determination of 4-chloroindole-3-acetic acid methyl ester in Lathyrus, Vicia and Pisum by gas chromatography-mass spectrometry. Physiol Plant 48: 499–503

    Google Scholar 

  16. Garcia-Martinez JL, Sponsel VM and Gaskin P (1987) Gibberellins in developing fruits of Pisum sativum cv Alaska: Studies on their role in pod growth and seed development. Planta 170: 130–137

    Google Scholar 

  17. Gates PJ, Yarwood JN, Harris N, Smith ML and Boulter ME (1981) Cellular changes in the pedicel and peduncle during flower abscission in Vicia faba. In R Thompson, ed. Vicia faba: Physiology and Breeding, pp. 299–316. The Hague: Martinus Nijhoff

    Google Scholar 

  18. Gates PJ, Smith ML and Boulter D (1983) Reproductive physiology of Vicia faba. In: PD Hebblethwaite, ed. The Faba Bean, pp. 133–142. London: Butterworth

    Google Scholar 

  19. Gehriger W and Keller ER (1979) Einfluss des Köpfens der Triebe auf die Versorgung der Blüten bei der Ackerbohne (Vicia faba L.) mit 14C. Schweiz landw Forschung 18: 59–80

    Google Scholar 

  20. Goodwin BP (1978) Phytohormones and fruit growth. In: DS Letham, PB Goodwin and TJV Higgins, eds. Phytohormones and Related Compounds-A Comprehensive Treatise, Vol. II, pp. 175–214. Amsterdam: Elsevier/North Holland Biomedical Press

    Google Scholar 

  21. Hofinger M and Böttger M (1979) Identification by GC-MS of 4-chloroindolylacetic acid and its methyl ester in immature Vicia faba seeds. Phytochem 18: 653–654

    Article  Google Scholar 

  22. Huff A and Dybing CD (1980) Factors affecting shedding of flowers in soybean (Glycine max L.). J Exp Bot 31: 751–762

    Google Scholar 

  23. Jaquiery R and Keller ER (1978) Beeinflussung des Fruchtansatzes bei der Ackerbohne (Vicia faba L.) durch die Verteilung der Assimilate, Teil I. Angew Botanik 52: 261–276

    Google Scholar 

  24. Jaquiery R and Keller ER (1980) Beeinflussung des Fruchtansatzes bei der Ackerbohne (Vicia faba L.) durch die Verteilung der Assimilate, Teil II. Angew Botanik 54: 29–39

    Google Scholar 

  25. Kambal AE (1969) Flower drop and fruit set in field beans, Vicia faba L. J Agric Sci 72: 131–138

    Google Scholar 

  26. Keller ER (1974) Die Ackerbohne (Vicia faba L.)-eine vergessene Kulturpflanze mit Zukunftsaussichten? Schweiz Landw Forschung 13: 287–300

    Google Scholar 

  27. Kellerhals M and Keller ER (1983) Effect of plant growth regulator combinations on faba bean development and yield components. In: PD Hebblethwaite, TCK Dawkins, MC Heath and G Lockwood, eds. Vicia faba: Agronomy, Physiology and Breeding 1984, pp. 37–45. The Hague: Martinus Nijhoff

    Google Scholar 

  28. Kellerhals M and Keller ER (1984) Effect of plant growth regulator combinations on fruit abscission in Vicia faba L. Fabis Newsletter 10: 8–10

    Google Scholar 

  29. Knegt E and Bruinsma J (1973) A rapid, sensitive and accurate determination of indolyl-3-acetic acid. Phytochem 12: 753–756

    Article  Google Scholar 

  30. McEwen J (1973) The effects of growth regulators, seed rates and row spacings on field beans (Vicia faba L.). J Agric Sci 80: 37–42

    Google Scholar 

  31. Pharis RP and King RW (1985) Gibberellins and reproductive development in seed plants. Ann Rev Plant Physiol 36: 517–568

    Google Scholar 

  32. Pless T, Böttger M, Hedden P and Graebe J (1984) Occurrence of 4-Cl-indoleacetic acid in broad beans and correlation of its levels with seed development. Plant Physiol 74: 320–323

    Google Scholar 

  33. Pommer G, Scheller H and Fink F (1980) Zur Ertragsbildung der Ackerbohne (Vicia faba L.) Bayer Landw Jb 57: 210–219

    Google Scholar 

  34. Quebedeaux B, Sweetser PB and Rowell JC (1976) Abscisic acid levels in soybean reproductive structures during development. Plant Physiol 58: 363–366

    Google Scholar 

  35. Ross GS, Minchin PEH and McWha JA (1987) Direct evidence of abscisic acid affecting phloem unloading within the seed coat of peas. J Plant Physiol 129: 435–441

    Google Scholar 

  36. Rowland GG, Bond DA and Parker ML (1983) Estimates of the frequency of fertilization in Field beans (Vicia faba L.). J Agric Sci 100: 25–33

    Google Scholar 

  37. Saftner RA and Wyse RE (1985) Effect of plant hormones on sucrose uptake by sugar beet root tissue discs. Plant Physiol 74: 951–955

    Google Scholar 

  38. Schussler JR, Brenner ML and Brun WA (1984) Abscisic acid and its relationship to seed filling in soybeans. Plant Physiol 76: 301–306

    Google Scholar 

  39. Sexton R, Lewis LN, Trewavas AJ and Kelley P (1984) Ethylene and abscission. In: JA Roberts and GA Tucker, eds. Ethylene and Plant Development (1985), pp. 173–196. London: Butterworth

    Google Scholar 

  40. Sharma YP, Vatsa VK and Agarwal K (1980) Influence of some growth regulators on the growth and yield of Vicia faba L. Indian J Bot 3: 76–78

    Google Scholar 

  41. Sjut V and Bangerth F (1984) Induced parthenocarpy-a way of manipulating levels of endogenous hormones in tomato fruits (Lycopersicon esculentum Mill.). 2. Diffusible hormones. Plant Growth Reg 2: 49–56

    Google Scholar 

  42. Stoessl A and Venis MA (1970) Determination of submicrogram levels of indole-3-acetic acid. Anal Biochem 34: 344–351

    PubMed  Google Scholar 

  43. Sponsel VM (1980) Gibberellin metabolism in legume seeds. In: JR Lenton, ed. Gibberellins-Chemistry, Physiology and Use, Monograph 5, pp. 49–62. Wantage: British Plant Growth Regulator Group

    Google Scholar 

  44. Sponsel VM, Gaskin P and MacMillan J (1979) The identification of gibberellins in immature seeds of Vicia faba, and some chemotaxonomic considerations. Planta 146: 101–105

    Google Scholar 

  45. Tamas IA, Wallace DH, Ludford PM and Ozbun JL (1979) Effect of older fruits on abortion and abscisic acid concentration of younger fruits in Phaseolus vulgaris L. Plant Physiol 64: 620–622

    Google Scholar 

  46. Tamas IA, Davies PJ, Mazur BK and Campbell LB (1985) Correlative effects of fruits on plant development. In: R. Shibles, ed. World Soybean Research Conference III: Proceedings (1985), pp. 858–865. Boulder and London: Westview Press

    Google Scholar 

  47. Van Steveninck RF (1957) Factors affecting the abscission of reproductive organs in yellow lupins (Lupinus luteus L.) I. The effect of different patterns of flower removal. J Exp Bot 8: 373–381

    Google Scholar 

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Author notes

  1. R. Diethelm

    Present address: Dept. of Agronomy, Iowa State University, 50011, Ames, IA, USA

Authors and Affiliations

  1. Institute of Plant Sciences, Swiss Federal Institute of Technology, CH-8092, Zurich, Switzerland

    R. Diethelm & E. R. Keller

  2. Institute of Horticulture, University of Hohenheim, D-7000, Stuttgart 70, FRG

    F. Bangerth

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  2. E. R. Keller
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  3. F. Bangerth
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Diethelm, R., Keller, E.R. & Bangerth, F. Auxins, ABA and gibberellin-like activity in abscising and non-abscising flowers and pods of Vicia faba L.. Plant Growth Regul 7, 75–90 (1988). https://doi.org/10.1007/BF00025203

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  • DOI: https://doi.org/10.1007/BF00025203

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