Summary
Twenty-five mulberry genotypes were studied for callus induction, to evaluate the effectiveness of hormones in promoting callus growth and to identify genotypes capable of regenerating plants. Fifteen genotypes showed callus initiation. Genotypic variation was also noted for longevity and rate of growth of callus cultures. Calli of different genotypes were maintained for more than one year. Frequency of callus initiation was high on Murashige & Skoog's modified medium incorporated with 2.0 mg/l 2,4-D, 100 mg/l casein acid hydrolysate and 150 ml/l coconut water. Regeneration through organogenesis was achieved in six genotypes indicating genotypic specificity.
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Susheelamma, B.N., Shekar, K.R., Sarkar, A. et al. Genotype and hormonal effects on callus formation and regeneration in mulberry. Euphytica 90, 25–29 (1996). https://doi.org/10.1007/BF00025156
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DOI: https://doi.org/10.1007/BF00025156