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Water stress and the catabolism of (±)-abscisic acid by excised leaves of Hordeum vulgare L. cv. Dyan

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Abstract

When excised, light-grown leaves of Hordeum vulgare were fed with (±)-[2-14C]-abscisic acid and stressed until they had lost 12% of their original fresh weight, marked changes in the distribution of radioactivity between abscisic acid and its catabolites were observed. Wilted leaves were less able than their turgid counterparts to transform (±)-[2-14C]-abscisic acid into 2′-hydroxymethyl abscisic acid, dihydrophaseic acid and water-soluble conjugates of abscisic acid. Water stress had little effect on the production of phaseic acid although refeeding studies with [14C]-phaseic acid showed that the step from phaseic acid to dihydrophaseic acid was inhibited in wilted leaves. Evidence was obtained which suggested that these changes did not result from dilution of applied, radiolabelled substrate by endogenous abscisic acid. The catabolites of (±)-abscisic acid were identified by capillary gas chromatography-mass spectrometry.

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References

  1. Bray, GA (1960) A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Anal Biochem 1: 279–285

    Google Scholar 

  2. Cornish, K, Zeevaart, JAD (1984) Abscisic acid metabolism in relation to water stress and leaf age in Xanthium strumarium. Plant Physiol 76: 1029–1035

    Google Scholar 

  3. Harrison, MA, Walton, DC (1975) Abscisic acid metabolism in water stressed bean leaves. Plant Physiol 56: 250–254

    Google Scholar 

  4. Loveys, BR, Brien, CJ, Kriedemann, PE (1975) Biosynthesis of abscisic acid under osmotic stress:Studies based on a dual labelling technique. Physiol Plant 33: 166–170

    Google Scholar 

  5. Mansfield, TA, Wellburn, AR, Moreira, TJS (1978) The role of abscisic acid and farnesol in the alleviation of water stress. Phil Trans R Soc London B 284: 471–482

    Google Scholar 

  6. Mertens, R, Stuning, M, Weiler, EW (1982) Metabolism of tritiated enantiomers of abscisic acid prepared by immunoaffinity chromatography. Naturwissenschaften 69: 595–597

    Google Scholar 

  7. Milborrow, BV (1978) The stability of conjugated abscisic acid during wilting. J exp Bot 29: 1059–1066

    Google Scholar 

  8. Milborrow BV (1983) Pathways to and from abscisic acid. In: FT Addicott, ed. Abscisic Acid, pp 79–111. Praeger Publishers

  9. Milborrow, BV, Noddle, RC (1970) Conversion of 5-(1,2-Epoxy-2,6,6-trimethylcyclohexyl)-3-methyl penta-cis-2-trans-4-dienoic acid into abscisic acid in plants. Biochem J 119: 727–734

    Google Scholar 

  10. Milborrow, BV, Robinson, DR (1973) Factors affecting the biosynthesis of abscisic acid. J exp Bot 24: 537–548

    Google Scholar 

  11. Murphy, GJP (1984) Metabolism of R,S-[2-14C] abscisic acid by non-stressed and water-stressed detached leaves of wheat (Triticum aestivum L.) Planta 160: 250–255

    Google Scholar 

  12. Quarrie, SA (1981) Genetic variability and heritability of drought-induced abscisic acid accumulation in spring wheat. Plant Cell Environ 4: 147–151

    Google Scholar 

  13. Railton, ID, Cowan, AK (1985) 2′-Hydroxymethyl abscisic acid: A major catabolite of (±)-abscisic acid in leaves of Hordeum vulgare L.. Plant Sci 42: 169–172

    Google Scholar 

  14. Railton, ID, Symon, B (1983) Detection of a new acidic metabolite of abscisic acid in leaves of Spinacia oleracea L cv Viroflay. Plant Sci Lett 28: 349–353

    Google Scholar 

  15. Sondheimer, E, Galson, EC, Chang, YP, Walton, DC (1971) Asymmetry, its importance to the action and metabolism of abscisic acid. Science 174: 829–831

    Google Scholar 

  16. Vaughan, GT, Milborrow, BV (1984) The resolution by HPLC of RS-[2-14C]Me 1′,4′-cis-diol of abscisic acid and the metabolism of (−)-R-and (+)-S-abscisic acid. J exp Bot 35: 110–120

    Google Scholar 

  17. Wright STC (1978) Phytochromes and stress phenomena. In: DS Letham et al., eds. Phytochromes and Related Compounds:A Treatise, Vol 2, pp 495–536. Elsevier/North Holland

  18. Zeevaart, JAD (1983) Metabolism of abscisic acid and its regulation in Xanthium leaves during and after water stress. Plant Physiol 71: 477–482

    Google Scholar 

  19. Zeevaart, JAD, Milborrow, BV (1976) Metabolism of abscisic acid and the occurrence of epi-dihydrophaseic acid in Phaseolus vulgaris. Phytochem 15: 493–500

    Google Scholar 

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Authors and Affiliations

  1. Plant Growth Laboratory, Department of Plant Sciences, Rhodes University, 6140, Grahamstown, South Africa

    Ian D. Railton & A. Keith Cowan

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  1. Ian D. Railton
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  2. A. Keith Cowan
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Railton, I.D., Cowan, A.K. Water stress and the catabolism of (±)-abscisic acid by excised leaves of Hordeum vulgare L. cv. Dyan. Plant Growth Regul 5, 115–124 (1987). https://doi.org/10.1007/BF00024739

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  • DOI: https://doi.org/10.1007/BF00024739

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